The Roles of White Adipose Tissue and Liver NADPH in Dietary Restriction-Induced Longevity DOI Creative Commons

Leah E. Jamerson,

Patrick C. Bradshaw

Antioxidants, Journal Year: 2024, Volume and Issue: 13(7), P. 820 - 820

Published: July 8, 2024

Dietary restriction (DR) protocols frequently employ intermittent fasting. Following a period of fasting, meal consumption increases lipogenic gene expression, including that NADPH-generating enzymes fuel lipogenesis in white adipose tissue (WAT) through the induction transcriptional regulators SREBP-1c and CHREBP. knockout mice, unlike controls, did not show an extended lifespan on DR diet. WAT cytoplasmic NADPH is generated by both malic enzyme 1 (ME1) pentose phosphate pathway (PPP), while liver primarily synthesized folate cycle provided one-carbon units serine catabolism. During daily fasting diet, fatty acids are released from transported to peripheral tissues, where they used for beta-oxidation phospholipid lipid droplet synthesis, monounsaturated (MUFAs) may activate Nrf1 inhibit ferroptosis promote longevity. Decreased PPP stimulated browning protected high-fat high levels macrophages linked obesity. But oscillations [NADPH]/[NADP+] feeding cycles play important role maintaining metabolic plasticity drive Studies measuring malate/pyruvate as proxy [NADPH]/[NADP+], well studies using fluorescent biosensors expressed animal models monitor changes needed during ad libitum diets determine associated with

Language: Английский

Histone deacetylases and their inhibitors in inflammatory diseases DOI Open Access

Sen-Yu Zhang,

Liying Zhang, Ri Wen

et al.

Biomedicine & Pharmacotherapy, Journal Year: 2024, Volume and Issue: 179, P. 117295 - 117295

Published: Aug. 14, 2024

Despite considerable research efforts, inflammatory diseases remain a heavy burden on human health, causing significant economic losses annually. Histone deacetylases (HDACs) play role in regulating inflammation (via histone and non-histone protein deacetylation) chromatin structure gene expression regulation. Herein, we present detailed description of the different HDACs their functions analyze diseases, including pro-inflammatory cytokine production reduction, immune cell function modulation, anti-inflammatory activity enhancement. Although HDAC inhibitors have shown broad disease treatment potentials, clinical applicability remains limited because non-specific effects, adverse drug resistance. With further insight, these are expected to become important tools for wide range diseases. This review aims explore mechanisms application prospects multiple

Language: Английский

Citations

13
Role of malate dehydrogenase 1 and isocitrate dehydrogenase 1 and their posttranslational modifications in diseases DOI
Chunxia Shi,

Yukun Wang,

Jin Guo

et al.

Biochemical and Biophysical Research Communications, Journal Year: 2025, Volume and Issue: 754, P. 151535 - 151535

Published: Feb. 24, 2025

Language: Английский

Citations

0
Embryonic lethal abnormal vision like 1-stabilized histone deacetylase 6 promotes hepatic stellate cell activation to accelerate liver fibrosis progression through ribosomal protein S5 downregulation DOI Open Access
Qin Wang, Wenjie Zhang, Jianping Wang

et al.

CytoJournal, Journal Year: 2025, Volume and Issue: 22, P. 30 - 30

Published: March 6, 2025

Histone deacetylase 6 (HDAC6) has been confirmed to participate in the regulation of liver fibrosis (LF) progression. This study aims explore role and mechanism HDAC6 LF process. Serum samples were collected from cirrhosis (LC) patients normal healthy individuals. Human hepatic stellate cells (HSC; LX-2) stimulated with transforming growth factor β1 (TGF-β1) mimic cell models. The levels HDAC6, ribosomal protein S5 (RPS5), embryonic lethal abnormal vision like 1 (ELAVL1), fibrosis-related markers determined by quantitative real-time polymerase chain reaction or western blot. Cell proliferation invasion detected using counting kit 8 assay, 5-ethynyl-2'-deoxyuridine Transwell assay. contents inflammatory factors examined enzyme-linked immunosorbent Furthermore, co-immunoprecipitation RNA immunoprecipitation assays performed assess interaction between RPS5 ELAVL1. effect ELAVL1 knockdown on mRNA stability was evaluated Actinomycin D treatment showed increased expression LC patients. reduced TGF-β1-induced LX-2 proliferation, invasion, fibrosis, inflammation. Moreover, acetylation RPS5, reversed inhibition si-HDAC6 Meanwhile, interacted stabilize its mRNA, thus inhibiting expression. Our data revealed that ELAVL1-stabilized promoted HSC activation repressing acetylation, providing a novel target for alleviating

Language: Английский

Citations

0
The Roles of White Adipose Tissue and Liver NADPH in Dietary Restriction-Induced Longevity DOI Creative Commons

Leah E. Jamerson,

Patrick C. Bradshaw

Antioxidants, Journal Year: 2024, Volume and Issue: 13(7), P. 820 - 820

Published: July 8, 2024

Dietary restriction (DR) protocols frequently employ intermittent fasting. Following a period of fasting, meal consumption increases lipogenic gene expression, including that NADPH-generating enzymes fuel lipogenesis in white adipose tissue (WAT) through the induction transcriptional regulators SREBP-1c and CHREBP. knockout mice, unlike controls, did not show an extended lifespan on DR diet. WAT cytoplasmic NADPH is generated by both malic enzyme 1 (ME1) pentose phosphate pathway (PPP), while liver primarily synthesized folate cycle provided one-carbon units serine catabolism. During daily fasting diet, fatty acids are released from transported to peripheral tissues, where they used for beta-oxidation phospholipid lipid droplet synthesis, monounsaturated (MUFAs) may activate Nrf1 inhibit ferroptosis promote longevity. Decreased PPP stimulated browning protected high-fat high levels macrophages linked obesity. But oscillations [NADPH]/[NADP+] feeding cycles play important role maintaining metabolic plasticity drive Studies measuring malate/pyruvate as proxy [NADPH]/[NADP+], well studies using fluorescent biosensors expressed animal models monitor changes needed during ad libitum diets determine associated with

Language: Английский

Citations

3