In Silico design of a multi-epitope vaccine for Human Parechovirus: Integrating immunoinformatics and computational techniques DOI Creative Commons

Arnob Sarker,

Md. Mahmudur Rahman, Chadni Khatun

et al.

PLoS ONE, Journal Year: 2024, Volume and Issue: 19(12), P. e0302120 - e0302120

Published: Dec. 4, 2024

Human parechovirus (HPeV) is widely recognized as a severe viral infection affecting infants and neonates. Belonging to the Picornaviridae family, HPeV categorized into 19 distinct genotypes. Among them, HPeV-1 most prevalent genotype, primarily associated with respiratory digestive symptoms. Considering HPeV’s role leading cause of life-threatening infections in lack effective antiviral therapies, our focus centered on developing two multi-epitope vaccines, namely HPeV-Vax-1 HPeV-Vax-2, using advanced immunoinformatic techniques. Multi-epitope vaccines have advantage protecting against various virus strains may be preferable live attenuated vaccines. Using NCBI database, three protein sequences (VP0, VP1, VP3) from six were collected construct consensus sequences. Then antigenicity, toxicity, allergenicity, stability analyzed after discovering T-cell linear B-cell epitopes The fundamental structures produced by fusing selected appropriate linkers adjuvants. Comprehensive physicochemical, antigenic, allergic assays, disulfide engineering demonstrated effectiveness Further refinement secondary tertiary models for both revealed promising interactions toll-like receptor 4 (TLR4) molecular docking, further confirmed dynamics simulation. In silico immunological modeling was employed assess vaccine’s capacity stimulate an immune reaction. simulations evaluate vaccines’ ability trigger response. Codon optimization cloning analyses showed that Escherichia coli (E . coli) likely host candidate Our findings suggest these could potential are recommended wet-lab investigation.

Language: Английский

In silico design of a multi-epitope vaccine against Mycobacterium avium subspecies paratuberculosis DOI Creative Commons
Wei Guo, Xinyu Wang, Jiangang Hu

et al.

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: Jan. 28, 2025

The widespread chronic enteritis known as Paratuberculosis (PTB) or Johne's disease (JD) is caused by Mycobacterium avium subspecies paratuberculosis (MAP), posing a significant threat to global public health. Given the challenges associated with PTB JD, development and application of vaccines are potentially important for control. aim this study was design multi-epitope vaccine against MAP. A total 198 MAP genomes were analyzed using pan-genome reverse vaccinology approaches. B-cell T-cell epitope analysis performed on selected promising cross-protective antigens followed selection epitopes high antigenicity, no allergenicity, toxicity vaccine. designed evaluated through molecular dynamics simulations, docking, immunological simulations. results revealed identification five antigens. In total, 10 epitopes, HTL 9 CTL Both candidate vaccine-TLR4 complex demonstrated considerable stability in Molecular docking studies confirmed that successfully interacted TLR4. Immunological simulations showed an increase both populations after vaccination. Additionally, exhibited codon adaptability index 1.0 GC content 53.64%, indicating strong potential successful expression Escherichia coli . This research developed targeting pan-genomes methods, offering innovative strategies creating effective

Language: Английский

Citations

1
A review on the development of bacterial multi-epitope recombinant protein vaccines via reverse vaccinology DOI
Yuxin Li, Muhammad Haris Raza Farhan, Xiaohan Yang

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 282, P. 136827 - 136827

Published: Oct. 30, 2024

Language: Английский

Citations

6
Innovative epitopes in Staphylococcal Protein-A an immuno-informatics approach to combat MDR-MRSA infections DOI Creative Commons

Pengjun Zhou,

Xingming Shi, Jinquan Xia

et al.

Frontiers in Cellular and Infection Microbiology, Journal Year: 2025, Volume and Issue: 14

Published: Jan. 14, 2025

Methicillin-resistant Staphylococcus aureus (MRSA) poses a significant challenge in clinical environments due to its resistance standard antibiotics. Staphylococcal Protein A (SpA), crucial virulence factor of MRSA, undermines host immune responses, making it an attractive target for vaccine development. This study aimed identify potential epitopes within SpA that could elicit robust ultimately contributing the combat against multidrug-resistant (MDR) MRSA. The protein sequence was retrieved from UniProt database, with various bioinformatics tools employed epitope prediction. T-cell were identified using Tepitool server, focusing on high-affinity interactions prevalent human leukocyte antigens (HLAs). B-cell predicted BepiPred tool. Predicted underwent docking studies HLA molecules evaluate binding properties. In-silico analyses confirmed antigenicity, promiscuity, and non-glycosylated nature selected epitopes. Several T B cell identified, showcasing high affinities extensive population coverage. multi-epitope construct, linked by synthetic linkers adjuvant, modelled, refined, validated through assessments. candidate subsequently docked Toll-like receptor 4 (TLR-4) immunogenicity. lays groundwork developing epitope-based vaccines targeting identifying promising candidates experimental validation innovative immunotherapeutic strategies MRSA infections.

Language: Английский

Citations

0
Design of Multi-Epitope Chimeric Phage Nanocarrier Vaccines for Porcine Deltacoronavirus DOI

Guoqing Zhao,

Yumin Zhang, Yan Li

et al.

Veterinary Microbiology, Journal Year: 2025, Volume and Issue: unknown, P. 110487 - 110487

Published: March 1, 2025

Language: Английский

Citations

0
In vivo immunogenicity assessment of a multiepitope‐displayed phage vaccine against Brucella species infection in BALB/c mice DOI Creative Commons
Mahmood Fadaie, Mahsa Esmaeilifallah, Anis Khalafiyan

et al.

Bioengineering & Translational Medicine, Journal Year: 2025, Volume and Issue: unknown

Published: April 25, 2025

Abstract Bacteriophages are considered ideal vaccine platforms owing to their safety, intrinsic adjuvant properties, stability, and low‐cost production. One of the best strategies prevent brucellosis in humans animals is vaccination. For several years, researchers have dedicated efforts enhance effectiveness safety Brucella vaccine. This study was designed evaluate immunogenicity a phage displaying multiepitopes from six different protective proteins mouse model. used immunoinformatics predict T‐ B‐cell epitopes. Subsequently, multiepitope protein synthesized recombinant phages were prepared. The display on confirmed by Western blot analysis. Six groups BALB/c mice (6 per group) received (as vaccine), helper phage, PBS as controls subcutaneously or orally. An ELISA assay analyze humoral response serum, while an interferon‐gamma ELISpot performed splenocytes cell‐mediated immune response. Mice immunized with showed significant serum levels specific IgG numbers IFN‐producing T cells splenic lymphocytes ( p ‐value <0.05). oral administration route provided much stronger cellular than subcutaneous injection (about 10‐fold), which important for combating infection. These findings provide first evidence that multiepitope‐displayed may be promising avenue developing safe efficient against species.

Language: Английский

Citations

0
In Silico design of a multi-epitope vaccine for Human Parechovirus: Integrating immunoinformatics and computational techniques DOI Creative Commons

Arnob Sarker,

Md. Mahmudur Rahman, Chadni Khatun

et al.

PLoS ONE, Journal Year: 2024, Volume and Issue: 19(12), P. e0302120 - e0302120

Published: Dec. 4, 2024

Human parechovirus (HPeV) is widely recognized as a severe viral infection affecting infants and neonates. Belonging to the Picornaviridae family, HPeV categorized into 19 distinct genotypes. Among them, HPeV-1 most prevalent genotype, primarily associated with respiratory digestive symptoms. Considering HPeV’s role leading cause of life-threatening infections in lack effective antiviral therapies, our focus centered on developing two multi-epitope vaccines, namely HPeV-Vax-1 HPeV-Vax-2, using advanced immunoinformatic techniques. Multi-epitope vaccines have advantage protecting against various virus strains may be preferable live attenuated vaccines. Using NCBI database, three protein sequences (VP0, VP1, VP3) from six were collected construct consensus sequences. Then antigenicity, toxicity, allergenicity, stability analyzed after discovering T-cell linear B-cell epitopes The fundamental structures produced by fusing selected appropriate linkers adjuvants. Comprehensive physicochemical, antigenic, allergic assays, disulfide engineering demonstrated effectiveness Further refinement secondary tertiary models for both revealed promising interactions toll-like receptor 4 (TLR4) molecular docking, further confirmed dynamics simulation. In silico immunological modeling was employed assess vaccine’s capacity stimulate an immune reaction. simulations evaluate vaccines’ ability trigger response. Codon optimization cloning analyses showed that Escherichia coli (E . coli) likely host candidate Our findings suggest these could potential are recommended wet-lab investigation.

Language: Английский

Citations

3