A chromosome-level genome assembly of yellow stem borer (Scirpophaga incertulas)

Scientific Data, Journal Year: 2024, Volume and Issue: 11(1)

Published: March 8, 2024

The yellow stem borer Scirpophaga incertulas is the dominant pest of rice in tropical Asia. However, lack genomic resources makes it difficult to understand their invasiveness and ecological adaptation. A high-quality chromosome-level genome S. incertulas, a monophagous pest, was assembled by combining Illumina short reads, PacBio HiFi long sequencing, Hi-C scaffolding technology. final size 695.65 Mb, with scaffold N50 28.02 93.50% sequences were anchored 22 chromosomes. BUSCO analysis demonstrated that this assembly had high level completeness, 97.65% gene coverage. total 14,850 protein-coding genes 366.98 Mb transposable elements identified. In addition, comparative analyses indicated chemosensory processes detoxification capacity may play critical roles specialized host preference incertulas. summary, provides valuable genetic resource for understanding biological characteristics its developing an efficient management strategy.

Language: Английский

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Invasive Drosophila suzukii outnumbers native controphics and causes substantial damage to fruits of forest plants

NeoBiota, Journal Year: 2022, Volume and Issue: 77, P. 39 - 77

Published: Oct. 18, 2022

Impacts of biological invasions are diverse and can have far-reaching consequences for ecosystems. The spotted wing drosophila, Drosophila suzukii , is a major invasive pest fruits, which negatively affects fruit wine production. However, little known about the ecological impact this fly species on more natural ecosystems it has invaded, such as forests. In study, we investigated use potential host plants by D. at 64 sites in different forest communities Switzerland from mid-June to mid-October 2020. We examined than 12,000 fruits egg deposits assess its direct plants. recorded symptoms decay after deposition determine if attacks trigger decay. addition, monitored drosophilid fauna with cup traps baited apple cider vinegar, expected that would outnumber potentially outcompete native controphics, especially other drosophilids. Egg were found 31 39 plant studied, 18 showing an attack rate > 50%. Overall, Cotoneaster divaricatus (96%), Atropa bella-donna (91%), Rubus fruticosus corylifolius aggr. Frangula alnus (85%) Sambucus nigra (83%) attacked particularly frequently, resulting also high predicted probabilities varied among communities. Later longer fruiting, black colour, larger size higher pulp pH all positively affected rates. More 50% showed severe deposition, sugar content leading symptoms. observed was reflected abundance dominance trap catches, independent community elevation. far most abundant species, accounting 86% (81,395 individuals) associated Our results indicate competes strongly frugivorous presence might ecosystem-level consequences. rapid leads loss resources may disrupt seed-dispersal mutualisms through reduced consumption dispersers birds.

Language: Английский

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Evolution of the odorant-binding protein gene family in Drosophila

Frontiers in Ecology and Evolution, Journal Year: 2022, Volume and Issue: 10

Published: Aug. 9, 2022

Odorant-binding proteins (OBPs) are encoded by a gene family involved in the perception of olfactory signals insects. This chemosensory has been advocated as candidate to mediate host preference and shifts insects, although it also participates other physiological processes. Remarkable differences OBP repertoire have described across insect groups, suggesting an accelerated turnover rate. The genus Drosophila , is valuable resource for ecological genomics studies since comprises groups ecologically diverse species there genome data many them. Here, we investigate molecular evolution this 19 genomes, including melanogaster repleta which mostly associated with rotting fruit cacti, respectively. We compared among closely related group, different subfamilies Cactaceae that represent disparate chemical challenges flies. found size varies widely between species, ranging from 39 54 OBPs. Indeed, more than 54% these genes organized clusters located on chromosomes X, 2, 5, distribution conserved throughout genus. sizes group D. virilis ( - radiation) were smaller group. tested alternative evolutionary models rates based scenarios. heterogeneous (GR) comparisons involving columnar cactus specialists, prickly pear dwellers lineages, rapid compatible positive selection specific genes. Taking ours previous results together, propose adaptation hypothesize adoption cactophilic lifestyle members family.

Language: Английский

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Functional Diversification, Redundancy, and Epistasis among Paralogs of the Drosophila melanogaster Obp50a–d Gene Cluster

Molecular Biology and Evolution, Journal Year: 2021, Volume and Issue: 38(5), P. 2030 - 2044

Published: Jan. 6, 2021

Large multigene families, such as the insect odorant-binding proteins (OBPs), are thought to arise through functional diversification after repeated gene duplications. Whereas many OBPs function in chemoreception, members of this family also expressed tissues outside chemosensory organs. Paralogs Obp50 cluster metabolic and male reproductive tissues, but their functions interrelationships remain unknown. Here, we report genetic dissection four cluster, which close physical proximity without intervening genes. We used CRISPR technology excise entire while introducing a PhiC31 reintegration site reinsert constructs different combinations constituent Obp genes were either intact or rendered inactive. performed whole transcriptome sequencing assessed sexually dimorphic changes transcript abundances (transcriptional niches) associated with each gene-edited genotype. Using approach, able estimate redundancy, additivity, diversification, epistasis among paralogs. analyzed effects editing on organismal phenotypes found significant skewing sex ratios attributable Obp50a, sex-specific starvation stress resistance Obp50d. Thus, there is within Obp50a contributing development Obp50d resistance. The deletion-reinsertion approach applied provides general paradigm for paralogs families.

Language: Английский

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Genome-wide identification and expression pattern analysis of novel chemosensory genes in the German cockroach Blattella germanica

Genomics, Journal Year: 2022, Volume and Issue: 114(2), P. 110310 - 110310

Published: Feb. 11, 2022

The German cockroach Blattella germanica is an important urban insect pest worldwide. In many insects, chemosensation essential for guiding their behaviors survival. Although a large number of chemosensory-related genes have been identified in B. germanica, little information on tissue-specific and developmental expression patterns has not uncovered yet. this study, we performed transcriptome analysis different tissues to reveal novel chemosensory proteins (CSPs) sensory neuron membrane (SNMPs). addition, phylogenetic tree gender-specific multiple gene families analyzed. We three CSPs (BgerCSP11, BgerCSP12, BgerCSP13) five SNMP germanica. Tissue-specific profiling showed that CSP1, 8, 9 exhibited significant levels both adult 5th instar nymph antennae. results paved the way further functional study mechanism provided potential insecticide targets.

Language: Английский

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Olfactory genomics within the Lepidoptera

Elsevier eBooks, Journal Year: 2020, Volume and Issue: unknown, P. 469 - 505

Published: Sept. 25, 2020

Language: Английский

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Shortened lifespan induced by a high-glucose diet is associated with intestinal immune dysfunction in Drosophila sechellia

Journal of Experimental Biology, Journal Year: 2022, Volume and Issue: 225(21)

Published: Oct. 13, 2022

ABSTRACT Organisms can generally be divided into two nutritional groups: generalists that consume various types of food and specialists specific food. However, it remains unclear how adapt to only limited conditions in nature. In this study, we addressed question by focusing on Drosophila fruit flies. The generalist melanogaster a wide variety foods contain high glucose levels. contrast, the specialist sechellia consumes Indian mulberry, known as noni (Morinda citrifolia), which contains relatively little glucose. We showed lifespan D. was significantly shortened under high-glucose diet, but effect not observed for melanogaster. sechellia, diet induced disorganization gut epithelia visceral muscles, associated with abnormal digestion constipation. RNA-sequencing analysis revealed many immune-responsive genes were suppressed fed compared those control diet. Consistent difference expression genes, glucose-induced phenotypes restored addition tetracycline or scopoletin, major component noni, each suppresses bacterial growth. propose that, impairs immune function, leads change microbiota, epithelial structure lifespan.

Language: Английский

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Potential attractants from three host plants of Bactrocera dorsalis (Hendel) (Diptera: Tephritidae)

International Journal of Tropical Insect Science, Journal Year: 2022, Volume and Issue: 43(1), P. 107 - 114

Published: Nov. 28, 2022

Language: Английский

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Investigating the phylogenetic history of toxin tolerance in mushroom‐feeding Drosophila

Ecology and Evolution, Journal Year: 2023, Volume and Issue: 13(12)

Published: Dec. 1, 2023

Abstract Understanding how and when key novel adaptations evolved is a central goal of evolutionary biology. Within the immigrans‐tripunctata radiation Drosophila , many mushroom‐feeding species are tolerant host toxins, such as cyclopeptides, that lethal to nearly all other eukaryotes. In this study, we used phylogenetic functional approaches investigate evolution cyclopeptide tolerance in . First, inferred relationships among 48 using 978 single copy orthologs. Our results resolved previous incongruities within groups across phylogeny. Second, expanded on studies toxin by assaying 16 these for α‐amanitin found six them could develop diet with toxin. Finally, asked might have radiation, was ancestral subsequently lost multiple times. findings expand our understanding emphasize uniqueness adaptive complexity biochemical adaptations.

Language: Английский

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Seven Questions on the Chemical Ecology and Neurogenetics of Resource-Mediated Speciation

Frontiers in Ecology and Evolution, Journal Year: 2021, Volume and Issue: 9

Published: Feb. 18, 2021

Adaptation to different environments can result in reproductive isolation between populations and the formation of new species. Food resources are among most important environmental factors shaping local adaptation. The chemosensory system, ubiquitous sensory channel animal kingdom, not only detects food their chemical composition, but also mediates sexual communication many taxa. Chemosensory divergence may thus play a crucial role resource-mediated adaptation speciation. Understanding how system facilitate ecological speciation requires integrating mechanistic studies with studies, link genetics physiology properties divergent In this review, we use examples insect research present seven key questions that be used understand consumer populations.

Language: Английский

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