Odorant-Binding Proteins and Chemosensory Proteins in Spodoptera frugiperda: From Genome-Wide Identification and Developmental Stage-Related Expression Analysis to the Perception of Host Plant Odors, Sex Pheromones, and Insecticides

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(6), P. 5595 - 5595

Published: March 15, 2023

Spodoptera frugiperda is a worldwide generalist pest with remarkable adaptations to environments and stresses, including developmental stage-related behavioral physiological adaptations, such as diverse feeding preferences, mate seeking, pesticide resistance. Insects' odorant-binding proteins (OBPs) chemosensory (CSPs) are essential for the chemical recognition during responses or other processes. The genome-wide identification gene expression patterns of all these identified OBPs CSPs across S. have not been reported. Here, we screened SfruOBPs SfruCSPs, analyzed SfruCSPs repertoires stages sexes. We found 33 22 in genome. majority SfruOBP genes were most highly expressed adult male female stages, while more SfruCSP larval egg indicating their function complementation. revealed strong correlations respective phylogenic trees, correlation between evolution. In addition, chemical-competitive binding widely protein, SfruOBP31, host plant odorants, sex pheromones, insecticides. Further ligands assay broad functional related spectrum SfruOBP31 insecticides, suggesting its potential food, These results provide guidance future research on development regulators environmentally friendly pest-control strategies.

Language: Английский

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Variations in the expression of odorant binding and chemosensory proteins in the developmental stages of whitefly Bemisia tabaci Asia II-1

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: July 1, 2024

Abstract The cotton whitefly, Bemisia tabaci , is considered as a species complex with 46 cryptic species, Asia II-1 being predominant in Asia. This study addresses significant knowledge gap the characterization of odorant-binding proteins (OBPs) and chemosensory (CSPs) II-1. We explored expression patterns OBPs CSPs throughout their developmental stages compared motif these proteins. Significant differences were observed for 14 B. II-1, OBP8 CSP4 showing higher across stages. Phylogenetic analysis reveals that form distinct clades, appearing to be an ancestral gene, giving rise evolution other . genomic distribution highlights gene clustering on chromosomes, suggesting functional conservation evolutionary events following birth-and-death model. Molecular docking studies indicate strong binding affinities various odour compounds like β-caryophyllene, α-pinene, β-pinene limonene, reinforcing roles host recognition reproductive functions. elaborates our understanding putative different hitherto unexplored. dynamics interactions offer scope developing innovative methods controlling this global invasive pest.

Language: Английский

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The seminal odorant binding protein Obp56g is required for mating plug formation and male fertility in Drosophila melanogaster

eLife, Journal Year: 2023, Volume and Issue: 12

Published: Dec. 21, 2023

In Drosophila melanogaster and other insects, the seminal fluid proteins (SFPs) male sex pheromones that enter female with sperm during mating are essential for fertility induce profound post-mating effects on physiology. The SFPs in D. taxa include several members of large gene family known as odorant binding (Obps). Work has shown some Obp genes highly expressed antennae can mediate behavioral responses to odorants, potentially by carrying these molecules receptors. These observations have led hypothesis Obps might act molecular carriers or compounds important fertility, though functional evidence any species is lacking. Here, we used genetics test role seven response (PMR). We found Obp56g required induction PMR, whereas six dispensable. males lacking fail form a plug mated female's reproductive tract, leading ejaculate loss reduced storage, likely due its expression ejaculatory bulb. also examined evolutionary history genes, studies documented rapid evolution turnover SFP across taxa. extensive lability copy number positive selection acting two Obp22a Obp51a. Comparative RNAseq data from tract multiple revealed shows high subset taxa, conserved head phylogeny. Together, suggest may been co-opted function over time.

Language: Английский

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Genome-Wide Identification of the Genes of the Odorant-Binding Protein Family Reveal Their Role in the Olfactory Response of the Tomato Leaf Miner (Tuta absoluta) to a Repellent Plant

Agronomy, Journal Year: 2024, Volume and Issue: 14(1), P. 231 - 231

Published: Jan. 22, 2024

The remarkable biological and evolutionary adaptations of insects to plants are largely attributed the powerful chemosensory systems insects. tomato leaf miner (Tuta absoluta) is a destructive invasive pest with global distribution that poses serious threat production nightshade crops, especially tomatoes. Functional can attract or repel insect pests by releasing volatiles interact olfactory system insects, thereby reducing damage target crops. However, there limited research on interaction between T. absoluta genes functional plants. In this study, 97 members putative odorant-binding protein (OBP) family have been identified in whole genome absoluta. Phylogenetic analysis involving various Lepidopteran Dipteran species, including D. melanogaster, revealed OBP gene families present conserved clustering patterns. Furthermore, Plus-C subfamily showed extremely significant expansion. Moreover, expression levels varied significantly different developmental stages; is, highest number were expressed adult stage, followed larval fewer high abundance egg stage. On other hand, through Y-tube olfactometer, we plant—Plectranthus tomentosa—that repels Finally, screened response P. tomentosa at genomic level using RT-qPCR. These results laid good foundation for controlling further studying genes.

Language: Английский

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Antennal transcriptome analysis reveals sensory receptors potentially associated with host detection in the livestock pest Lucilia cuprina

Parasites & Vectors, Journal Year: 2024, Volume and Issue: 17(1)

Published: July 18, 2024

Abstract Background Lucilia cuprina (Wiedemann, 1830) (Diptera: Calliphoridae) is the main causative agent of flystrike sheep in Australia and New Zealand. Female flies lay eggs an open wound or natural orifice, developing larvae eat host’s tissues, a condition called myiasis. To improve our understanding host-seeking behavior, we quantified gene expression male female antennae based on their behavior. Methods A spatial olfactometer was used to evaluate olfactory response L. mated males gravid females fresh rotting beef. Antennal RNA-Seq analysis identify sensory receptors differentially expressed between groups. Results were more attracted rotten compared beef (> fivefold increase). However, some did not respond either type performed dissected from females, non-attracted males. Transcripts encoding 11 families identified above threshold (≥ 5 transcript per million) including 49 ATP-binding cassette transporters (ABCs), two ammonium (AMTs), 37 odorant (ORs), 16 ionotropic (IRs), gustatory (GRs), 22 odorant-binding proteins (OBPs), 9 CD36-sensory neuron membrane (CD36/SNMPs), 4 chemosensory (CSPs), myeloid lipid-recognition (ML) Niemann-Pick C2 disease (ML/NPC2), 2 pickpocket (PPKs) 3 transient receptor potential channels (TRPs). Differential analyses sex-biased receptors. Conclusions We that both sexes hence may be associated with host detection by flies. The most promising for future investigations as follows: (LcupOR46) which female-biased Cochliomyia hominivorax Coquerel, 1858; ABC transporter (ABC G23.1) sole upregulated females; ammonia (AMT_Rh50), previously Drosophila melanogaster Meigen, 1830. This first report suggesting possible role olfaction potentially other insects. Graphical

Language: Английский

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Unravelling the genomic landscape reveals the presence of six novel odorant-binding proteins in whitefly Bemisia tabaci Asia II-1

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 279, P. 135140 - 135140

Published: Aug. 30, 2024

Language: Английский

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Decoding the genomic terrain: functional insights into 14 chemosensory proteins in whitefly Bemisia tabaci Asia II-1

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Nov. 1, 2024

Genome-wide analysis of Bemisia tabaci Asia II-1 unravelled for the first-time full-length sequences 14 chemosensory proteins (CSPs), their exon-intron boundaries, insertion sites retrotransposons, and clustering patterns on chromosomes. All CSPs sans CSP6 have an N-terminal signal peptide. The presence OS-D superfamily PhBP domains in different suggests roles transduction pheromone binding. Motif reveals conservation cohesiveness hemiptera. phylogenetic uncovers evolutionary lineages Hemipteran CSPs. RT-qPCR showed spatial expression body tissues B. adults. In-silico docking high-affinity binding CSP 1 5 with two insecticides, imidacloprid fipronil, energy values ranging from − 5.8 to -9.3 kcal/mol, along details interacting aminoacidic residues hydrophobic pockets these Further functional validation was done through insecticide bioassays RNAi. This study provides novel insights into genomic architecture II-1, characterisation that CSP1 genes may indirect resistance. It lays foundation further research developing new control strategies tabaci.

Language: Английский

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Genetic analyses suggest that larval and adult stages ofLucilia cuprinaemploy different sensory systems

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 22, 2024

Abstract The blowfly Lucilia cuprina is a destructive parasite of sheep that causes flystrike or myiasis. Larvae consume the animal’s living flesh, producing large wounds can lead to death. Growing resistance conventional control methods has prompted analysis alternative strategies. RNA-Seq whole larvae at different stages and third instar head gut tissues, suggested odorant (OR), gustatory, ionotropic pickpocke t receptors may not play central role in L. larval sensory signaling digestive systems. Rather, ABC transporters were highly enriched RNA, OBPs only head. To confirm ORs are essential for detection rotten beef, diet-choice assays performed including adults homozygous null mutation coreceptor ( LcupOrco ) gene. While attraction adult females beef was fully disrupted, mutant showed no change diet preference.

Language: Английский

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The seminal odorant binding protein Obp56g is required for mating plug formation and male fertility inDrosophila melanogaster

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Feb. 7, 2023

Abstract In Drosophila melanogaster and other insects, the seminal fluid proteins (SFPs) male sex pheromones that enter female with sperm during mating are essential for fertility induce profound post-mating effects on physiology behavior. The SFPs in D. taxa include several members of large gene family known as odorant binding (Obps). Previous work has shown some Obp genes highly expressed antennae can mediate behavioral responses to odorants, potentially by carrying these molecules receptors. These observations have led hypothesis Obps might act molecular carriers or compounds important ejaculate, though functional evidence any species is lacking. Here, we used RNAi CRISPR/Cas9 generated mutants test role seven response (PMR). We found Obp56g required induction PMR, whereas six had no effect when mutated individually. male’s ejaculatory bulb, an tissue reproductive tract synthesizes components plug. males lacking fail form a plug mated female’s tract, leading ejaculate loss reduced storage. also examined evolutionary history genes, studies documented rapid evolution turnover SFP across taxa. extensive lability copy number positive selection acting two Obp22a Obp51a . Comparative RNAseq data from multiple revealed shows high expression only obscura groups, conserved head all tested. Together, suggest may been co-opted function over time.

Language: Английский

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