A gut commensal niche regulates stable association of a multispecies microbiota DOI Open Access
Ren Dodge, Eric W. Jones, Haolong Zhu

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2021, Volume and Issue: unknown

Published: Sept. 30, 2021

Abstract The intestines of animals are typically colonized by a complex, relatively stable microbiota that influences health and fitness, but the underlying mechanisms colonization remain poorly understood. As typical animal, fruit fly, Drosophila melanogaster, is associated with consistent set commensal bacterial species, yet reason for this consistency unknown. Here, we use gnotobiotic flies, microscopy, microbial pulse-chase protocols to show niche exists within proventriculus region foregut selectively binds bacteria exquisite strain-level specificity. Primary colonizers saturate exclude secondary same strain, initial Lactobacillus physically remodels favor Acetobacter . Our results provide mechanistic framework understanding establishment stability an intestinal microbiome. One-Sentence Summary A strain-specific inhabits defined spatial gut forms niche.

Language: Английский

A symbiotic physical niche in Drosophila melanogaster regulates stable association of a multi-species gut microbiota DOI Creative Commons
Ren Dodge, Eric W. Jones, Haolong Zhu

et al.

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: March 21, 2023

Abstract The gut is continuously invaded by diverse bacteria from the diet and environment, yet microbiome composition relatively stable over time for host species ranging mammals to insects, suggesting host-specific factors may selectively maintain key of bacteria. To investigate specificity, we used gnotobiotic Drosophila , microbial pulse-chase protocols, microscopy stability different strains in fly gut. We show that a host-constructed physical niche foregut binds with strain-level stabilizing their colonization. Primary colonizers saturate exclude secondary same strain, but initial colonization Lactobacillus physically remodels through production glycan-rich secretion favor unrelated commensals Acetobacter genus. Our results provide mechanistic framework understanding establishment multi-species intestinal microbiome.

Language: Английский

Citations

45
Involvement of Microbiota in Insect Physiology: Focus on B Vitamins DOI Creative Commons
Javier Serrato-Salas, Mathilde Gendrin

mBio, Journal Year: 2022, Volume and Issue: 14(1)

Published: Dec. 13, 2022

Insects are highly successful in colonizing a wide spectrum of ecological niches and feeding on diversity diets. This is notably linked to their capacity get from microbiota any essential component lacking the diet such as vitamins amino acids. Over century research based dietary analysis, antimicrobial treatment, gnotobiotic rearing, culture-independent microbe detection progressively generated wealth information about role specific aspects insect fitness. Thanks recent increase sequencing capacities, whole-genome number symbionts has facilitated tracing biosynthesis pathways, validation experimental data evolutionary analyses. field considerable set hosts harboring or nonspecific members. Here, we review current knowledge involvement tick nutrition, with particular focus B vitamin provision. We specifically question if there specificity provision by compared redundant yet contribution microbes. successively highlight known microbial during three main life stages invertebrates: postembryonic development, adulthood, reproduction.

Language: Английский

Citations

39
Impact of intraspecific variation in insect microbiomes on host phenotype and evolution DOI Creative Commons
Claudia Lange, Stéphane Boyer, Т. Martijn Bezemer

et al.

The ISME Journal, Journal Year: 2023, Volume and Issue: 17(11), P. 1798 - 1807

Published: Sept. 2, 2023

Abstract Microbes can be an important source of phenotypic plasticity in insects. Insect physiology, behaviour, and ecology are influenced by individual variation the microbial communities held within insect gut, reproductive organs, bacteriome, other tissues. It is becoming increasingly clear how microbiome for fitness, expansion into novel ecological niches, environments. These investigations have garnered heightened interest recently, yet a comprehensive understanding intraspecific assembly function these insect-associated shape insects still lacking. Most research focuses on core associated with species ignores variation. We argue that among driver evolution, we provide examples showing such influence fitness health insects, invasions, their persistence new environments, responses to global environmental changes.

Language: Английский

Citations

32
Delivery mechanism can enhance probiotic activity against honey bee pathogens DOI Creative Commons
Brendan A. Daisley, Andrew P. Pitek,

Christina Torres

et al.

The ISME Journal, Journal Year: 2023, Volume and Issue: 17(9), P. 1382 - 1395

Published: June 14, 2023

Managed honey bee (Apis mellifera) populations play a crucial role in supporting pollination of food crops but are facing unsustainable colony losses, largely due to rampant disease spread within agricultural environments. While mounting evidence suggests that select lactobacilli strains (some being natural symbionts bees) can protect against multiple infections, there has been limited validation at the field-level and few methods exist for applying viable microorganisms hive. Here, we compare how two different delivery systems-standard pollen patty infusion novel spray-based formulation-affect supplementation three-strain consortium (LX3). Hives pathogen-dense region California supplemented 4 weeks then monitored over 20-week period health outcomes. Results show both facilitate uptake LX3 adult bees, although do not colonize long-term. Despite this, treatments induce transcriptional immune responses leading sustained decreases many opportunistic bacterial fungal pathogens, as well selective enrichment core including Bombilactobacillus, Bifidobacterium, Lactobacillus, Bartonella spp. These changes ultimately associated with greater brood production growth relative vehicle controls, no apparent trade-offs ectoparasitic Varroa mite burdens. Furthermore, spray-LX3 exerts potent activities Ascosphaera apis (a deadly pathogen) likely stemming from in-hive dispersal differences, whereas patty-LX3 promotes synergistic development via unique nutritional benefits. findings provide foundational basis probiotic application apiculture collectively highlight importance considering method management strategies.

Language: Английский

Citations

31
Microbiome-derived acidity protects against microbial invasion in Drosophila DOI Creative Commons
Alexander J. Barron, Sneha Agrawal, Danielle Lesperance

et al.

Cell Reports, Journal Year: 2024, Volume and Issue: 43(4), P. 114087 - 114087

Published: April 1, 2024

Microbial invasions underlie host-microbe interactions resulting in pathogenesis and probiotic colonization. In this study, we explore the effects of microbiome on microbial invasion Drosophila melanogaster. We demonstrate that gut microbes Lactiplantibacillus plantarum Acetobacter tropicalis improve survival lead to a reduction burden during infection. Using interaction assay, report L. inhibits growth invasive bacteria, while A. reduces inhibition. further show inhibition by is linked its ability acidify environment via lactic acid production lactate dehydrogenase, diminishes quenching acids. propose from gatekeeper invasions, as only capable tolerating acidic environments can colonize host. The methods findings described herein will add growing breadth tools study microbe-microbe broad contexts.

Language: Английский

Citations

10
How commensal microbes shape the physiology of Drosophila melanogaster DOI Creative Commons
Théodore Grenier, François Leulier

Current Opinion in Insect Science, Journal Year: 2020, Volume and Issue: 41, P. 92 - 99

Published: Aug. 14, 2020

Language: Английский

Citations

63
Allometric releases of nitrogen and phosphorus from sediments mediated by bacteria determines water eutrophication in coastal river basins of Bohai Bay DOI Creative Commons
Jiayuan Liu, Yue Feng, Yan Zhang

et al.

Ecotoxicology and Environmental Safety, Journal Year: 2022, Volume and Issue: 235, P. 113426 - 113426

Published: March 17, 2022

Although the Chinese government has conducted much work in recent years to reduce land-based pollutant discharge, eutrophication continues occur frequently many rivers, estuaries, and coastal waters. This may indicate that sediment is a major source rather than sink for nitrogen (N) phosphorus (P). To clarify endogenous mechanisms of river basins, status, physicochemical properties, bacterial parameters overlying waters sediments catchment (CA), estuarine (EA), offshore (OA) areas Duliujian River Basin Bohai Bay were investigated. The results showed index (EI) CA, EA, OA 62.71, 57.86, 36.51, respectively. EI was more sensitive increases P (slope = 3.887) N 0.734) water, indicating main factor driving basin. However, nonlinear relationship found between waters, suggesting mediation during release. As speculated this study, water increased quickly with increasing diversity metabolic abundance, allometric release mediated by bacteria risk eutrophication. Redundancy analysis organic matter total have positive effects on communities, which explains 21.8% 23.7% variation diversity, 31.3% 7.1% metabolism. also suggests accumulation promotes further aggravates water. Therefore, simultaneous control necessary basins.

Language: Английский

Citations

34
Bugs in Bugs: The Role of Probiotics and Prebiotics in Maintenance of Health in Mass-Reared Insects DOI Creative Commons
Carlotta Savio, Loretta Mugo-Kamiri, Jennifer Upfold

et al.

Insects, Journal Year: 2022, Volume and Issue: 13(4), P. 376 - 376

Published: April 11, 2022

Interactions between insects and their microbiota affect insect behaviour evolution. When specific microorganisms are provided as a dietary supplement, reproduction, food conversion growth enhanced health is improved in cases of nutritional deficiency or pathogen infection. The purpose this review to provide an overview insect-microbiota interactions, the role probiotics, general use reared for feed, interactions with host microbiota. We how bacterial strains have been selected species feed discuss methods used isolate measure effectiveness probiotic. outline future perspectives on probiotic applications mass-reared insects.

Language: Английский

Citations

32
Sensing of the non-essential amino acid tyrosine governs the response to protein restriction in Drosophila DOI
Hina Kosakamoto, Naoki Okamoto,

Hide Aikawa

et al.

Nature Metabolism, Journal Year: 2022, Volume and Issue: 4(7), P. 944 - 959

Published: July 25, 2022

Language: Английский

Citations

30
The Function and the Affecting Factors of the Zebrafish Gut Microbiota DOI Creative Commons
Pingping Li, Jinhua Zhang, Xiaoyi Liu

et al.

Frontiers in Microbiology, Journal Year: 2022, Volume and Issue: 13

Published: June 2, 2022

Gut microbiota has become a topical issue in unraveling the research mechanisms underlying disease onset and progression. As an important potential “organ,” gut plays role regulating intestinal epithelial cell differentiation, proliferation, metabolic function immune response, angiogenesis host growth. More recently, zebrafish models have been used to study interactions between hosts. It several advantages, such as short reproductive cycle, low rearing cost, transparent larvae, high genomic similarity humans, easy construction of germ-free (GF) transgenic zebrafish. In our review, we reviewed large amount data focusing on close relationship health. Moreover, outlined functions function, response. More, summarized major factors that can influence composition, abundance, diversity microbiota, which will help us understand significance biological provide options for maintaining balance

Language: Английский

Citations

27