Sexual dimorphic distribution of G protein‐coupled receptor 30 in pain‐related regions of the mouse brain DOI
You Li, Zhenhua Jiang, Wenqiang Zuo

et al.

Journal of Neurochemistry, Journal Year: 2023, Volume and Issue: unknown

Published: Nov. 4, 2023

Abstract Sex differences in pain sensitivity have contributed to the fact that medications for curing chronic are unsatisfactory. However, underlying mechanism remains be elucidated. Brain‐derived estrogen participates modulation of sex and related emotion. G protein‐coupled receptor 30 (GPR30), identified as a novel with different distribution than traditional receptors, has been proved play vital role regulating affected by estrogen. contribution its sexually dimorphic pain‐related behaviors not fully explored. In current study, immunofluorescence assays were applied mark neurons expressing GPR30 male female mice (in metestrus proestrus phase) brain regions. The express CaMKIIα or VGAT also labeled observe overlap GPR30. We found females had more GPR30‐positive (GPR30 + ) primary somatosensory (S1) insular cortex (IC) males. lateral habenula (LHb) nucleus tractus solitarius (NTS), males females. Moreover, within LHb, expression varied estrous cycle phase; fewer those proestrus. addition, neurons, which co‐expressed medial preoptic (mPOA) males, while basolateral complex amygdala (BLA). These findings may partly explain modulatory effects emotional phenotypes between sexes provide basis comprehension sexual dimorphism GPR30, finally new targets exploiting treatments sex‐specific pain.

Language: Английский

HCN channels in the lateral habenula regulate pain and comorbid depressive‐like behaviors in mice DOI Creative Commons
Xuezhong Cao, Mengye Zhu, Gang Xǔ

et al.

CNS Neuroscience & Therapeutics, Journal Year: 2024, Volume and Issue: 30(7)

Published: July 1, 2024

Comorbid anxiodepressive-like symptoms (CADS) in chronic pain are closely related to the overactivation of lateral habenula (LHb). Hyperpolarization-activated cyclic nucleotide-gated (HCN) channels have been implicated play a key role regulating neuronal excitability. However, HCN LHb during CADS has not yet characterized. This study aimed investigate effect on pain.

Language: Английский

Citations

5
Machines, mathematics, and modules: the potential to provide real-time metrics for pain under anesthesia DOI Creative Commons
Ke Peng, Keerthana Deepti Karunakaran, Stephen Green

et al.

Neurophotonics, Journal Year: 2024, Volume and Issue: 11(01)

Published: Feb. 22, 2024

The brain-based assessments under anesthesia have provided the ability to evaluate pain/nociception during surgery and potential prevent long-term evolution of chronic pain. Prior studies shown that functional near-infrared spectroscopy (fNIRS)-measured changes in cortical regions such as primary somatosensory polar frontal cortices show consistent response evoked ongoing pain awake, sedated, anesthetized patients. We take this basic approach integrate it into a framework could provide real-time measures peri-surgical period. This application significant implications for providing analgesia surgery, practice currently lacks quantitative evidence guide patient tailored management. Through simple readout “pain” or “no pain,” proposed system diminish eliminate levels intraoperative, early post-operative, potentially, transition post-surgical system, when validated, also be applied analgesic efficacy clinic.

Language: Английский

Citations

4
Sleep Deprivation Triggers Mitochondrial DNA Release in Microglia to Induce Neural Inflammation: Preventative Effect of Hydroxytyrosol Butyrate DOI Creative Commons
Yachong Hu, Yongyao Wang, Yifang Wang

et al.

Antioxidants, Journal Year: 2024, Volume and Issue: 13(7), P. 833 - 833

Published: July 12, 2024

Sleep deprivation (SD) triggers mitochondrial dysfunction and neural inflammation, leading to cognitive impairment mental issues. However, the mechanism involving inflammation still remains unclear. Here, we report that SD rats exhibited multiple behavioral disorders, brain oxidative stress, robust DNA (mtDNA) oxidation. In particular, activated microglia microglial mtDNA efflux cytosol provoked pro-inflammatory cytokines. We observed cytokines significantly reduced with suppression of With treatment a novel nutrient, hydroxytyrosol butyrate (HTHB), SD-induced disorders were ameliorated while oxidation, release, NF-κB activation remarkably alleviated in both rat N9 cell line. Together, these results indicate oxidation resultant release induced by mediate HTHB prevents efflux, providing potential for

Language: Английский

Citations

4
Pde4b-regulated cAMP signaling pathway in the AUDGABA-S1TrSst circuit underlies acute-stress-induced anxiety-like behavior DOI Creative Commons

Zhixin Xiao,

Xiaoya Wang,

Nan Zhou

et al.

Cell Reports, Journal Year: 2025, Volume and Issue: 44(2), P. 115253 - 115253

Published: Jan. 30, 2025

Language: Английский

Citations

0
Spinal TAOK2 contributes to neuropathic pain via cGAS-STING activation in rats DOI Creative Commons
Hui Zhang, Ang Li, Yu‐Fan Liu

et al.

iScience, Journal Year: 2023, Volume and Issue: 26(10), P. 107792 - 107792

Published: Aug. 30, 2023

Thousand and one amino acid kinase 2 (TAOK2) is a member of the mammalian sterile 20 family implicated in neurodevelopmental disorders; however, its role neuropathic pain remains unknown. Here, we found that TAOK2 was enriched activated after chronic constriction injury (CCI) rat spinal dorsal horn. Meanwhile, cyclic guanosine monophosphate-adenosine monophosphate synthase (cGAS)-stimulator interferon genes (STING) signaling also with hyperalgesia. Silencing reversed hyperalgesia suppressed activation cGAS-STING induced by CCI, while pharmacological hypersensitivity upregulation naive rats. Furthermore, inhibition or gene silencing attenuated CCI-induced Taken together, these data demonstrate contributes to via activation, providing new molecular targets for treatment pain.

Language: Английский

Citations

4
Combined Experiments with <i>in Vivo</i> Fiber Photometry and Behavior Tests Can Facilitate the Measurement of Neuronal Activity in the Primary Somatosensory Cortex and Hyperalgesia in an Inflammatory Pain Mice Model DOI Open Access
Tatsuya Ishikawa, Daisuke Uta, Hiroaki Okuda

et al.

Biological and Pharmaceutical Bulletin, Journal Year: 2024, Volume and Issue: 47(3), P. 591 - 599

Published: March 4, 2024

The pain matrix, which includes several brain regions that respond to sensation, contribute the development of chronic pain. Thus, it is essential understand mechanism causing in matrix such as anterior cingulate (ACC), or primary somatosensory (S1) cortex. Recently, combined experiment with behavior tests and vivo calcium imaging using fiber photometry revealed interaction between neuronal function deep including ACC phenotype However, remains unclear whether this can identify activity S1, receive behaviors hyperalgesia allodynia. In study, examine change S1 hind paw before after inflammatory was detected from same animal, system von Frey hairs test applied. This amplitude responses neurons increased mechanical threshold decreased animals have an Moreover, we found values thresholds were shifted negative correlation has a possibility simultaneously consider induced effects analgesics treatments.

Language: Английский

Citations

1
Map activation of various brain regions using different frequencies of electroacupuncture ST36, utilizing the Fos-CreER strategy DOI Creative Commons

Zi Guo,

Naixuan Wei,

Ru Ye

et al.

Acupuncture and Herbal Medicine, Journal Year: 2024, Volume and Issue: 4(3), P. 386 - 398

Published: March 6, 2024

Objective: Electroacupuncture (EA) is an alternative treatment option for pain. Different frequencies of EA have different pain-relieving effects; however, the central mechanism still not well understood. Methods: The Fos2A-iCreER (TRAP):Ai9 mice were divided into three groups (sham, 2 Hz, and 100 Hz). intraperitoneally injected with 4-hydroxytamoxifen (4-OHT) immediately after at Zusanli (ST36) 30 min to record activated neurons. One week later, sacrificed, number TRAP-treated neurons by in thalamus, amygdala, cortex, hypothalamus was determined. Results: In Hz more than did cingulate cortex area 1 (Cg1) primary somatosensory (S1), EAs differ from sham EA. upregulated insular (IC) secondary (S2) compared those elevated paraventricular thalamic nucleus (PV) ventrolateral (VL), significantly EA, showed no difference or frequently ventral posterolateral (VPL) Conclusions: Low-frequency ST36 effectively activates Cg1, S1, S2, IC, VPL, PV, VL. enhanced excitability aforementioned nuclei induced low-frequency may be related its superior efficacy neuropathological

Language: Английский

Citations

1
Electroacupuncture may alleviate inflammatory pain by inhibiting the expression of P2RY14 in the primary somatosensory cortex DOI
Shuai Hou,

Ruizhu Zhou,

Liuxuan He

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: July 24, 2024

Abstract Electroacupuncture (EA) has been successfully used to treat painful conditions. However, its analgesic mechanism remains poorly understood. Here, we established a Complete Freund's Adjuvant (CFA)-induced inflammatory pain model. After CFA injection, P2RY14 was upregulated in primary somatosensory cortex of hindlimb (S1HL). EA, not sham alleviated the nociceptive thermal hyperalgesia and reduced upregulation mice. Injection agonist uridine diphosphate glucose (UDPG) into bilateral S1HL reversed EA-induced analgesia. Our results demonstrate that analgesia CFA-induced can be achieved by downregulation S1HL.

Language: Английский

Citations

0
Chemogenetic inhibition of pain-related neurons in the posterior insula cortex reduces mechanical hyperalgesia and anxiety-like behavior during acute pain DOI Creative Commons
Ilia Potapenko, Tatsuya Ishikawa, Hiroaki Okuda

et al.

Biochemical and Biophysical Research Communications, Journal Year: 2024, Volume and Issue: 734, P. 150625 - 150625

Published: Aug. 29, 2024

Pain is a complex phenomenon that involves sensory, emotional, and cognitive components. The posterior insula cortex (pIC) has been shown to integrate multisensory experience with emotional states. However, the involvement of pIC in regulation affective behavior pain remains unclear. Here, we investigate role pain-related neurons anxiety-like during acute pain. We combined chemogenetic approach targeted recombination active populations (TRAP) mice. Global inhibition attenuates chemically-induced mechanical hypersensitivity without affecting behavior. In contrast, reduces both present study provides important insights into sensory

Language: Английский

Citations

0
Identification of the shared gene MXD3 signatures and biological mechanism in patients with hip pain and prostate cancer DOI Creative Commons

Liang Huang,

Yu Xie,

Shusuan Jiang

et al.

Medicine, Journal Year: 2024, Volume and Issue: 103(37), P. e39592 - e39592

Published: Sept. 13, 2024

Prostate cancer (PRAD) is recognized as having a significant effect on systemic illnesses. This study examined possible immune cells, metabolic pathways, and genes that may explain the interaction between PRAD hip pain. We used information retrieved from Cancer Genome Atlas Gene Expression Omnibus databases. To find common genes, we utilized differential expression analysis weighted gene co-expression network analysis. The were shared subjected to pathway enrichment studies using Ontology Kyoto Encyclopedia of Genes Genomes. Additionally, hub analyzed LASSO regression, receiver operating characteristic curve was generated based screening outcomes. for nodes chosen in protein–protein built. Single-sample gene-set performed identify differentially expressed genes. Immunohistochemistry staining confirmed expression, single-sample assessed cell infiltration. concluded by comparing MAX dimerization protein 3 (MXD3) interactor 1 (MXI1) tumor tissues Uniform Manifold Approximation Projection violin plots Tumor lmmune Single-cell Hub database. After analyzing intersection analysis-significant module determined MXD3 best diagnostic biomarker One potential predictor development MXI1 node gene, which found network. analyses revealed had relatively positive correlation with neutrophil T-helper infiltration levels, whereas negative mast Tgd levels. Tumors lower levels higher compared normal tissues. Endothelial induced pluripotent stem smooth muscle cells all express MXI1. first investigate close genetic link pain bioinformatics technologies. 2 most involved crosstalk immunological responses triggered T neutrophils be crucial relationship

Language: Английский

Citations

0