Novel in situ base editing of gut bacteria - inconsistencies, gaps, and anticipated dangers on treated humans, animals, and the entire ecosystem DOI Open Access
Siguna Mueller

Published: July 30, 2024

Recent years have seen an enormous rise in the “editing” of genomes living organisms. In openenvironment, this has raised concern unwanted mutations and adverse effects on non-targeted species. Aiming for a previously unattained goal, very recent publication Nature reports situ base editing targeted bacterial populations mouse gut. The genetic modifications such bacteria rely novel delivery system via synthetic virus. Such bacteriophages been engineered to target different receptors improve efficacy complex hybrid plasmid that carries gene editor. This DNA cosmid is predicted be non-replicative “prevent” maintenance dissemination payload. authors report successful gene-editing model pathogens, including some pathogenic strains.This paper gives independent logical appraisal design editor, experiments conducted, their interpretation. exposes several inconsistencies with study oddities which may, part, stem from fact are seemingly unaware key dangers editors risk assessment, related work demonstrates danger microbial evolution open environment turn, foster emergence difficulty conducting population analyses.The analysis also raises issues not mentioned paper, indicating potential serious consequences exposed non-target organisms or, when applied clinically, adulterate makeup treated human or animal. addition potentially systemic transgenes products derived them, urgent questions involve type these - resemble dangerous gain-of-function viruses even though they seem evade being defined as such. Beyond grave humans animals, well entire biosphere, legal gap lack appropriate biosafety biosecurity oversight may enable illicit technologies clandestine biological weapons programs.

Language: Английский

Inhibitory effects of benzyl isothiocyanate on widespread mcr-1-harbouring IncX4 plasmid transfer DOI Creative Commons

Sirirat Luk-in,

Kamonrat Phopin,

Sasina Bangmuangngam

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: April 15, 2025

Language: Английский

Citations

0
Existing and emerging mRNA vaccines and their environmental impact: a transdisciplinary assessment DOI Creative Commons
Siguna Mueller

Environmental Sciences Europe, Journal Year: 2024, Volume and Issue: 36(1)

Published: Aug. 12, 2024

mRNA vaccines have played a massive role during the COVID-19 pandemic and are now being developed for numerous other human animal applications. Nevertheless, their potential ramifications on environment lack scrutiny regulation. On 14 July 2020, EU decided to temporarily exclude clinical trials with from prior environmental risk assessment. Even though billions of doses been administered large-scale agricultural wildlife RNA applications fast-tracked, there is no knowledge impact via dispersion vaccine-derived material or wastage. This gap targeted here critical assessment (1) pharmacokinetic properties these products; (2) microbiota; (3) novel factors exemplified by gut bacterium Escherichia coli resulting in pathogen evolution guts wild animals, (4) findings mRNA-LNP platforms that implicate extracellular vesicles (EVs) as superior carriers, (5) potentials exogenous regulatory RNAs. analysis results first extrapolation (a) magnitude likelihood characterized FDA 2015 products facilitate action transcription and/or translation transferred genetic related processes, (b) additional risks facilitated horizontal transfer short The arguments provided establish rationale bioactive dispersed EVs, impacted microbiota, exposed organisms foster evolution, cross-species biological function, driving widespread ecosystem disturbances. Evidence emerging molecules, when ingested, could survive digestion mediate gene expression regulation, host–parasite defense, immunity, responses consuming animals. Highlighting further unresolved questions, comprehensive calls open dialogue more in-depth studies get clear picture globally most effectively gauge existing human, livestock, technologies weapons forms misuse. Regulatory measures urgently needed mitigate potentially damage public health well adverse societal, economic, legal implications.

Language: Английский

Citations

2
Existing and Emerging mRNA Vaccines and their Environmental Impact - a Transdisciplinary Assessment DOI Open Access
Siguna Mueller

Published: March 28, 2024

mRNA vaccines have played a momentous role during the COVID-19 pandemic and are now being developed for numerous other human animal applications. Yet, their potential ramifications on environment lack scrutiny regulation. On 14 July 2020, EU decided to temporarily exclude clinical trials with from prior environmental risk assessment. Even though billions of doses been administered large-scale agricultural wildlife RNA applications arefast-tracked, there is no knowledge impact via dispersion vaccine-derived material or wastage.This gap targeted here critical assessment (1) pharmacokinetic properties these products, (2) microbiota, (3) novel factors exemplified by gut bacterium Escherichia coli resulting in pathogen evolution guts wild animals, (4) findings mRNA-LNP platforms that implicate extracellular vesicles (EVs) as superior carriers, (5) potentials exogenous regulatory RNAs. Thereby, I obtain (a) first extrapolation magnitude likelihood characterized FDA 2015 products facilitate action transcription and/or translation transferred genetic related processes, (b) additional risks facilitated horizontal transfer short RNAs.This analysis establishes rationale bioactive dispersed EVs, impacted exposed organisms foster evolution, cross-species biological function, driving widespread ecosystem disturbances. Evidence emerging molecules, when ingested, could survive digestion mediate gene expression regulation, host-parasite defense, immunity, responses consuming animals. Highlighting further unresolved questions, anticipate this be starting point open dialogue more in-depth studies get clear picture globally, most effectively gauge existing human, livestock, technologies, weapons forms misuse. Regulatory measures urgently needed mitigate potentially damage public health well adverse societal, economic, legal implications.

Language: Английский

Citations

1
Prevalence, antimicrobial resistance and detection of virulence genes of Escherichia coli and Salmonella spp. isolated from white-lipped peccaries and collared peccaries DOI
Adriana Marques Faria, Itallo Conrado Sousa Araújo, Lorena Lopes Ferreira

et al.

Brazilian Journal of Microbiology, Journal Year: 2024, Volume and Issue: 55(2), P. 2035 - 2041

Published: May 7, 2024

Language: Английский

Citations

0
Novel in situ base editing of gut bacteria - inconsistencies, gaps, and anticipated dangers on treated humans, animals, and the entire ecosystem DOI Open Access
Siguna Mueller

Published: July 30, 2024

Recent years have seen an enormous rise in the “editing” of genomes living organisms. In openenvironment, this has raised concern unwanted mutations and adverse effects on non-targeted species. Aiming for a previously unattained goal, very recent publication Nature reports situ base editing targeted bacterial populations mouse gut. The genetic modifications such bacteria rely novel delivery system via synthetic virus. Such bacteriophages been engineered to target different receptors improve efficacy complex hybrid plasmid that carries gene editor. This DNA cosmid is predicted be non-replicative “prevent” maintenance dissemination payload. authors report successful gene-editing model pathogens, including some pathogenic strains.This paper gives independent logical appraisal design editor, experiments conducted, their interpretation. exposes several inconsistencies with study oddities which may, part, stem from fact are seemingly unaware key dangers editors risk assessment, related work demonstrates danger microbial evolution open environment turn, foster emergence difficulty conducting population analyses.The analysis also raises issues not mentioned paper, indicating potential serious consequences exposed non-target organisms or, when applied clinically, adulterate makeup treated human or animal. addition potentially systemic transgenes products derived them, urgent questions involve type these - resemble dangerous gain-of-function viruses even though they seem evade being defined as such. Beyond grave humans animals, well entire biosphere, legal gap lack appropriate biosafety biosecurity oversight may enable illicit technologies clandestine biological weapons programs.

Language: Английский

Citations

0