Efficient and high-density immobilization of animal cells by a microfiber with both swelling and cell adhesion properties and its application to exosome production DOI Creative Commons
Naofumi Shiomi, Pengfei Zhang,

Shinji Nakatuka

et al.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: March 25, 2025

Abstract Purpose For high-density cell culture, we studied the development of optimal microfibers (MFs) with a 0.1–10 µm diameter, which due to their large surface area can serve as an immobilization carrier for animal cells. To date, few studies have used MFs scaffolding culturing. Results Using six types nonsoluble synthetic polymers, MF sheets were fabricated by electrospinning. The cellulose acetate, polyketone, and polyvinyl acetate exhibited swelling water retention capacities. Next, fragments examined immobilizing TKD2 mouse vascular endothelial Although most cells taken into three characterized swelling, leaked from without adhesion. solve this, comprising polyketones coated gelatin. adhesive capacity was enhanced, decreased almost all immobilized remained on sheets’ surfaces. Based these results, produced novel sheet gelatin, polyketone mixture (CPG). Since attached CPG fragment supplied little loss reached high density 3.2×109 MF-g− 1, Furthermore, continuously exosomes productivity 6–7×1010 particles ml− 1 after either 8h or 16h Conclusion CPG-based are expected wide range future applications, including exosome production

Language: Английский

Recent advances and future prospects of engineered exosomes as advanced drug and gene delivery systems DOI
Waleed Y. Rizg, Majed Alghamdi, Sherif El Saadany

et al.

Journal of Drug Delivery Science and Technology, Journal Year: 2025, Volume and Issue: 106, P. 106696 - 106696

Published: Feb. 6, 2025

Language: Английский

Citations

1
Endothelial cell-derived exosomes trigger a positive feedback loop in osteogenesis-angiogenesis coupling via up-regulating zinc finger and BTB domain containing 16 in bone marrow mesenchymal stem cell DOI Creative Commons
Lu Liu, Nan Zhou, Songning Fu

et al.

Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)

Published: Nov. 19, 2024

The close spatial and temporal connection between osteogenesis angiogenesis around type H vasculature is referred as "osteogenesis-angiogenesis coupling", which one of the basic mechanisms osteogenesis. Endothelial cells (ECs), bone marrow mesenchymal stem (BMSCs), their specific lineage constitute important cluster that participate in regulation microenvironment. However, regulatory mechanism osteogenesis-angiogenesis coupling under condition healing has not been unveiled. In this study, we demonstrated exosome derived from ECs (EC-exo) an initiator blood vessels formation, EC-exo acts a mediator orchestrating by enhancing BMSC osteogenic differentiation EC both monolayer stereoscopic co-culture system primary human cells. transcriptome array indicated zinc finger BTB domain containing 16 (ZBTB16) key gene EC-exo-mediated osteogenesis, ZBTB16 indispensable EC-exo-initiated coupling. Mechanistically, up-regulated expression BMSCs, thereby promoting osteoprogenitor phenotype transformation; osteoprogenitors further promote vessel (H-ECs) generation activating HIF-1α pathway; H-ECs conversely promotes BMSCs. crosstalk BMSCs triggered constitutes positive feedback loop enhances This study demonstrates can become effective therapeutic tool to regeneration repair.

Language: Английский

Citations

4
Efficient and high-density immobilization of animal cells by a microfiber with both swelling and cell adhesion properties and its application to exosome production DOI Creative Commons
Naofumi Shiomi, Pengfei Zhang,

Shuji Nakatsuka

et al.

Biotechnology Letters, Journal Year: 2025, Volume and Issue: 47(2)

Published: April 1, 2025

Language: Английский

Citations

0
Efficient and high-density immobilization of animal cells by a microfiber with both swelling and cell adhesion properties and its application to exosome production DOI Creative Commons
Naofumi Shiomi, Pengfei Zhang,

Shinji Nakatuka

et al.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: March 25, 2025

Abstract Purpose For high-density cell culture, we studied the development of optimal microfibers (MFs) with a 0.1–10 µm diameter, which due to their large surface area can serve as an immobilization carrier for animal cells. To date, few studies have used MFs scaffolding culturing. Results Using six types nonsoluble synthetic polymers, MF sheets were fabricated by electrospinning. The cellulose acetate, polyketone, and polyvinyl acetate exhibited swelling water retention capacities. Next, fragments examined immobilizing TKD2 mouse vascular endothelial Although most cells taken into three characterized swelling, leaked from without adhesion. solve this, comprising polyketones coated gelatin. adhesive capacity was enhanced, decreased almost all immobilized remained on sheets’ surfaces. Based these results, produced novel sheet gelatin, polyketone mixture (CPG). Since attached CPG fragment supplied little loss reached high density 3.2×109 MF-g− 1, Furthermore, continuously exosomes productivity 6–7×1010 particles ml− 1 after either 8h or 16h Conclusion CPG-based are expected wide range future applications, including exosome production

Language: Английский

Citations

0