Biomimetic intelligent nanoplatform with cascade amplification effect for tumor synergy therapy DOI Creative Commons
Ying Wang,

Qing Ji,

Chao Yan

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Dec. 28, 2024

Tumor heterogeneity, immune-suppressive microenvironment and the precise killing of tumor cells by drugs are important factors affecting treatment. In this study, we developed environment-responsive drug delivery system (FM@IQ/PST&ZIF-8/DOX) based on ZIF-8 for photothermal/immunotherapy/chemotherapy synergistic therapy. The prepared FM@IQ/PST&ZIF-8/DOX nanoplatfrom not only has highly loading capacity chemotherapeutic drug-doxorubicin, but also erythrocyte membrance modified their surface can endow immunity-escaping property prolong blood circulation time. More important, neurotransmitter serotonin was encapsulated ZIF-8/DOX oxidative polymerization, which effectively avoid premature leakage DOX in circulation. And formed polyserotonin shell superior photothermal conversion performance, as well adsorption utilized to load imiqumod. When entered tissue, folate molecules specifically bind receptors improve uptake cells. vitro vivo results showed that nanoplatform could generate a large amount heat under near-infrared light irradiation, then induce apoptosis cells, release associated antigens, solve problem heterogeneity. addition, loaded imiquimod immunosuppressive microenvironment, enhance body's anti-tumor immune response, inhibit metastasis recurrence. Therefore, novel designed research achieve controllable release, it is expected become promising new strategy treatment provide corresponding inspiration later development drugs.

Language: Английский

Current advance of nanotechnology in diagnosis and treatment for malignant tumors DOI Creative Commons

Bilan Wang,

Shiqi Hu, Yan Teng

et al.

Signal Transduction and Targeted Therapy, Journal Year: 2024, Volume and Issue: 9(1)

Published: Aug. 12, 2024

Cancer remains a significant risk to human health. Nanomedicine is new multidisciplinary field that garnering lot of interest and investigation. shows great potential for cancer diagnosis treatment. Specifically engineered nanoparticles can be employed as contrast agents in diagnostics enable high sensitivity high-resolution tumor detection by imaging examinations. Novel approaches labeling are also made possible the use nanoprobes nanobiosensors. The achievement targeted medication delivery therapy accomplished through rational design manufacture nanodrug carriers. Nanoparticles have capability effectively transport medications or gene fragments tissues via passive active targeting processes, thus enhancing treatment outcomes while minimizing harm healthy tissues. Simultaneously, context radiation sensitization photothermal enhance therapeutic efficacy malignant tumors. This review presents literature overview summary how nanotechnology used According oncological diseases originating from different systems body combining pathophysiological features cancers at sites, we most recent developments applications. Finally, briefly discuss prospects challenges cancer.

Language: Английский

Citations

87
Pre-metastatic niche: formation, characteristics and therapeutic implication DOI Creative Commons
Yuhang Wang,

Jiachi Jia,

Fuqi Wang

et al.

Signal Transduction and Targeted Therapy, Journal Year: 2024, Volume and Issue: 9(1)

Published: Sept. 25, 2024

Language: Английский

Citations

22
KLF12 transcriptional activation by a novel LncRNA A930015D03Rik enhances melanoma metastasis DOI
Meng Xiang, Ziyi Li, Jie Su

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: 309, P. 142686 - 142686

Published: April 4, 2025

Language: Английский

Citations

0
Unleashing the therapeutic potential of tumor-draining lymph nodes: spotlight on bladder cancer DOI Creative Commons

Xin Yang,

Xiangyu Wu, Xinbao Hao

et al.

Journal of Translational Medicine, Journal Year: 2025, Volume and Issue: 23(1)

Published: April 29, 2025

Tumor-draining lymph nodes (TDLNs) are often involved during the metastasis of bladder cancer (BC), which is associated with a poor prognosis. Recent studies have shown that TDLNs major source host anti-tumor immunity, can impede tumor progression and favor immunotherapy. However, progression, various tumor-derived mediators modulate TDLN microenvironment, impairing their protective function. Ultimately, provide soil for proliferation dissemination cells. Therefore, surgical removal commonly recommended in solid tumors to prevent metastasis, but this poses significant challenges leveraging Additionally, node dissection (LND) has not survival benefits some tumors. Hence, decision remove oncological treatment needs be reconsidered. Herein, we spotlight BC introduce how cells stromal immune shape an immunosuppressive microenvironment progression. We summarize existing therapeutic strategies reinvigorate immunity TDLNs. Furthermore, discuss whether preserve role LND treatment.

Language: Английский

Citations

0
Characterizing spatial immune architecture in metastatic melanoma using high-dimensional multiplex imaging DOI Creative Commons
Joel Eliason, Santhoshi Krishnan, Yasunari Fukuda

et al.

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: April 29, 2025

Introduction Immune checkpoint inhibitors (ICIs) have significantly improved survival for patients with metastatic melanoma, yet many experienceresistance due to immunosuppressive mechanisms within the tumor immune microenvironment (TIME). Understanding how spatial architecture of and inflammatory components changes across disease stages may reveal novel prognostic biomarkers therapeutic targets. Methods We performed high-dimensional profiling two melanoma tissue microarrays (TMAs), representing Stage III ( n = 157) IV 248) tumors. Using imaging mass cytometry (IMC) multiplex immunofluorescence (mIF), we characterized phenotypic, functional, properties TIME. Cellular neighborhoods were defined by marker expression, interactions between cells quantified using nearest-neighbor functions (G-cross). Associations assessed Cox proportional hazards models robust variance estimation. Results tumors exhibited a distinct landscape, increased CD74- MIF-enriched reduced iNOS-associated regions compared III. Cytotoxic T lymphocytes (CTLs) more prevalent in TIME, while B NK depleted. Spatial analysis revealed that CTL–Th cell, NK–T B–NK cell linked survival, whereas macrophage aggregation excessive B–Th clustering correlated worse outcomes. Organ-specific analyses showed CTL infiltration near predicted gastrointestinal metastases, lymph node skin metastases. Discussion Our results stage-specific shifts composition organization In advanced disease, emerge alongside localization, patterns coordination—particularly involving CTLs, cells, cells—strongly predicting survival. These findings highlight refine patient stratification guide combination immunotherapy strategies targeting

Language: Английский

Citations

0
Melanoma-derived cytokines and extracellular vesicles are interlinked with macrophage immunosuppression DOI Creative Commons
Shankar Suman, Wendy K. Nevala,

Alexey A. Leontovich

et al.

Frontiers in Molecular Biosciences, Journal Year: 2025, Volume and Issue: 11

Published: Jan. 22, 2025

Cytokines play a crucial role in mediating cell communication within the tumor microenvironment (TME). Tumor-associated macrophages are particularly influential regulation of immunosuppressive cytokines, thereby supporting metastasis. The upregulation Th2 cytokines cancer cells is recognized for its involvement suppressing anticancer immunity. However, association between these and tumor-secreted extracellular vesicles (EVs) remains poorly understood. Therefore, our objective was to investigate connection tumor-promoting melanoma-derived EVs. analysis from altered cytokine profile data showed that EVs upregulate expression naïve macrophages, contributing promotion tumor-supporting functions. Notably, many were also found be upregulated metastatic melanoma patients (n = 30) compared healthy controls 33). Overall, findings suggest strong secretory induction tumor-associated facilitates development an TME, metastasis through at both local systemic levels.

Language: Английский

Citations

0
Predictive Factors for Sentinel Lymph Node Positivity in Melanoma Patients—The Role of Liquid Biopsy, MicroRNA and Gene Expression Profile Panels DOI Open Access
Federico Venturi, Elisabetta Magnaterra, Biagio Scotti

et al.

Cancers, Journal Year: 2025, Volume and Issue: 17(8), P. 1281 - 1281

Published: April 10, 2025

The identification of predictive factors for sentinel lymph node (SLN) positivity in melanoma patients is crucial accurate staging, prognosis, and personalized therapeutic decisions. This review synthesizes recent advancements molecular clinicopathological predictors, with a particular focus on liquid biopsy gene expression profiling (GEP) tools. Emerging evidence highlights the significant role miRNAs progression, metastatic potential, lymphatic spread. Clinicopathological such as Breslow thickness, ulceration, mitotic rate remain critical, while GEP provides additional precision by uncovering tumor-specific pathways. By integrating these tools, clinicians can improve risk stratification, reduce unnecessary procedures, personalize management strategies.

Language: Английский

Citations

0
Melanoma-Derived Extracellular Vesicles Induce CD36-Mediated Pre-Metastatic Niche DOI Creative Commons
Shankar Suman, Wendy K. Nevala,

Alexey A. Leontovich

et al.

Biomolecules, Journal Year: 2024, Volume and Issue: 14(7), P. 837 - 837

Published: July 11, 2024

CD36 expression in both immune and non-immune cells is known to be directly involved cancer metastasis. Extracellular vesicles (EVs) secreted by malignant melanocytes play a vital role developing tumor-promoting microenvironments, but it unclear whether this mediated through CD36. To understand the of melanoma, we first analyzed SKCM dataset for clinical prognosis, evaluated percentage lymphatic fluid-derived EVs (LEVs), tested melanoma-derived increase induce M2-macrophage-like characteristics. Furthermore, performed multiplex immunofluorescence (MxIF) imaging analysis evaluate its colocalization with various other lymph node (LN) patients control subjects. Our findings show that cutaneous melanoma have worse prognosis high levels, higher total LEVs were found at baseline compared control. We also monocytic endothelial treated expressed more than untreated cells. can regulate immunosuppressive macrophage-like characteristics upregulating The spatial data tumor-involved sentinel LNs exhibit probability from LNs, was not statistically significant. Conclusively, our demonstrated plays controlling microenvironment LN, which promote formation protumorigenic niche.

Language: Английский

Citations

2
Metabolic insights into tumor lymph node metastasis in melanoma DOI
Jiayi Huang, Zixu Gao, Jiangying Xuan

et al.

Cellular Oncology, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 20, 2024

Language: Английский

Citations

2
Patient‐Derived Melanoma Immune‐Tumoroids as a Platform for Precise High throughput Drug Screening DOI Creative Commons
Juliana Santos Rosa Viegas, Sofia Almeida Costa, Sofia Dias

et al.

Advanced Science, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 30, 2024

Abstract In vitro models are crucial in cancer research, but they must truthfully mimic vivo tumors for clinical relevance. The development of unprecedent melanoma quadruple multicellular tumoroids (MCTs) is proposed comprising tumor cells, keratinocytes, fibroblasts, and monocytes that replicate architecture, microenvironment, secretome behavior. These MCTs 300 µm diameter secreted keratin collagen, showing complexity proportional to their cell combinations. Further, closely resembled terms cells organization, growth, progression, immune Drug screening using these demonstrated potential as patient‐derived platforms precision medicine. findings highlight the true value studying biology testing therapeutic interventions with greater relevance human disease.

Language: Английский

Citations

1