Bioactive Molecules from the Invasive Blue Crab Callinectes sapidus Exoskeleton: Evaluation of Reducing, Radical Scavenging, and Antitumor Activities

Marine Drugs, Journal Year: 2025, Volume and Issue: 23(1), P. 45 - 45

Published: Jan. 17, 2025

In recent years, the invasive Atlantic blue crab (Callinectes sapidus) has increased its spread throughout Mediterranean Sea, threatening native biodiversity and local economies. This study aimed to valorize C. sapidus sampled in Sicily by utilizing exoskeleton as a source of chitosan, astaxanthin, bio-phenolic compounds. These biomolecules were evaluated for their reducing, radical scavenging, antitumor activity. The ferric ion reducing antioxidant power (FRAP) free scavenging activity against 2,2-Diphenyl-1-picrylhydrazyl (DPPH) significantly higher chitosan (3.16 ± 0.10 mg AAE/g 8.1 µmol TE/g). No significant differences observed among tested 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS). Both compounds astaxanthin exhibited dose-dependent cytotoxicity on CaCo-2 (IC50 = 12.47 18 µg/mL) HepG2 10.25 1.26 cell lines, while only bio-phenols showed no cytotoxic effect differentiated cells up 20 µg/mL. findings highlight value by-products supporting circular economy, offering sustainable approach managing this species providing bioactive with promising medical nutraceutical applications.

Language: Английский

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Optimization of Aqueous Extraction Conditions for Bioactive Compounds and Antioxidant Properties of Overripe Banana (Musa acuminata) Using Response Surface Methodology

Journal of Agriculture and Food Research, Journal Year: 2025, Volume and Issue: unknown, P. 101775 - 101775

Published: Feb. 1, 2025

Language: Английский

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Comparative physicochemical, microbiological, pharmacological, and toxicological evaluations of different varieties of honey targeting wound repairment

Biocatalysis and Agricultural Biotechnology, Journal Year: 2025, Volume and Issue: unknown, P. 103544 - 103544

Published: Feb. 1, 2025

Language: Английский

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Bioinputs from Eugenia dysenterica DC. (Myrtaceae): Optimization of Ultrasound-Assisted Extraction and Assessment of Antioxidant, Antimicrobial, and Antibiofilm Activities

Molecules, Journal Year: 2025, Volume and Issue: 30(5), P. 1115 - 1115

Published: Feb. 28, 2025

By-products of fruit processing may contain bioactive compounds with potential application as bioinputs. This study optimized the ultrasound-assisted extraction (UAE) phenolic from by-products Eugenia dysenterica DC (Myrtaceae) to obtain bioinputs antioxidant, antimicrobial, and antibiofilm activities. The (seeds peels) were subjected UAE optimization using Box–Behnken design response surface methodology. Two optimal conditions defined: 1-plant material/solvent ratio 0.01 g/mL, time 40 min, ethanol content 30%; 2–0.19 39 46%. (liquid extract—LQE; lyophilized extract—LYE), obtained under conditions, tested for antioxidant activity (ABTS, FRAP, DPPH). LQE: 1633.13 µM Trolox/g, 1633.60 FeSO4/g 73.35 g sample/g DPPH; LYE: 1379.75 1692.09 83.35 DPPH. For antimicrobial activity, both extracts presented MBC < 62.5 mg/mL MIC 2.5 P. aeruginosa. LQE action S. coagulase (50 mg/mL) Streptococcus spp. (12.5 mg/mL); LYE aeruginosa mg/mL; 12.5 mg/mL), E. coli (25 mg/mL). by present

Language: Английский

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Phytochemical Characterization and Antioxidant Activity of Cajanus cajan Leaf Extracts for Nutraceutical Applications

Molecules, Journal Year: 2025, Volume and Issue: 30(8), P. 1773 - 1773

Published: April 15, 2025

Cajanus cajan (guandú) is a widely cultivated leguminous plant in Panama; however, its phytochemical composition remains underexplored. Traditionally used Asia and Africa for medicinal properties, the plant’s bioactive compounds responsible these benefits have not been fully identified. The profile antioxidant capacity of C. leaf extracts from Panama were characterized, highlighting their potential applications. Ethanolic obtained via ultrasonication analyzed through screening, confirming presence alkaloids, tannins, saponins, steroids. Spectrophotometric analysis revealed high total phenolic (71 mg g−1) flavonoid (30 contents. Antioxidant assays demonstrated significant 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical cation (ABTS+) inhibition 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity. Gas chromatography–mass spectrometry (GC-MS) identified 35 leaves first time, including lupeol (antimicrobial antitumor), lupenone (antidiabetic), squalene (antitumor antioxidant), tocopherol (antioxidant), β-amyrin (antibacterial anti-Alzheimer’s). These findings expand known cajan, supporting pharmaceutical, nutraceutical, agro-industrial potential. Moreover, this research provides foundation further studies on applications human health sustainable agriculture.

Language: Английский

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Impact of Photoselective Nets on Phenolic Composition and Antioxidant Capacity in Different Apple Cultivars Under the Same Edaphoclimatic Conditions

Molecules, Journal Year: 2025, Volume and Issue: 30(9), P. 1995 - 1995

Published: April 30, 2025

Phenolic compounds in apples provide significant health benefits, including antioxidant, and anti-inflammatory properties. The phenolic profile content are influenced by genetics, environmental factors, agricultural practices. Photoselective nets, which designed to filter specific wavelengths of light, might impact fruit quality content. This study aimed assess the effects photoselective nets on antioxidant capacity composition three different apple cultivars grown under same edaphoclimatic conditions. Five were selected. Fruits compared with unprotected fruits. Antioxidant was evaluated, profiles established Ultra-High Performance Liquid Chromatography coupled Time Flight-Mass Spectrometry (UHPLC-ToF-MS). results demonstrate a activities apples. Different net colors had distinct accumulation compounds, some increasing flavonoid production others reducing levels important acids. gray IRIDIUM® Red enhanced quercetin its derivatives, while chlorogenic acid showed general decline net-covered conditions, indicating possible dependence direct sunlight. responses also cultivar-dependent, Gala redlum showing largest reductions when protected nets. assays confirmed that potential cultivar-dependent manner. These findings suggest retention bioactive fruits be strategically managed selecting appropriate materials for cultivars.

Language: Английский

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Optimization of Microwave-Assisted Polyphenol Extraction and Antioxidant Activity from Papaya Peel Using Response Surface Methodology and Artificial Neural Network

Applied Food Research, Journal Year: 2024, Volume and Issue: 4(2), P. 100591 - 100591

Published: Nov. 7, 2024

Language: Английский

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Optimization of Ultrasonic-Assisted Extraction of Total Flavonoids from Uvaria chamae Root Bark by Response Surface Methodology and Its Antioxidant Activity

European Modern Studies Journal, Journal Year: 2024, Volume and Issue: 8(5), P. 186 - 202

Published: Nov. 4, 2024

This study focuses on the optimization of ultrasound-assisted extraction (UAE) total flavonoids from Uvaria chamae root bark. As optimisation method, Response Surface Methodology (RSM) was used to investigate effect independent variables process. The factors highlighted during UAE were temperature (°C) X1, sonication time (s) X2, and solid liquid ratio (mL.mg-1) X3; yield being expected response. maximum ef?ciency achieved with optimal condition at 55 °C, 20 min 40:1 mL.mg-1. At those conditions, 16.34 % (178.624±2.512) µg EQ/g extract obtained. Regarding antioxidant assessment, DPPH (2, 2-diphenyl-1-picrylhydrazyl) method used. It emerges this evaluation that exhibited highest values radical-scavenging assay inhibition concentration (IC50) value (0.075±0.001) mg/mL better than BHT taking as control IC50 (0.089± 0.022) compared CSE. experimental predicted in good agreement, which suggested is more ef?cient conventional solvent can be obtain an improved active ingredients.

Language: Английский

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Exploring the impact of microwaves, ultrasounds, UVA light, and X-rays on polyphenols in alginate–whey protein matrix–encapsulated red grape seed extract powder

Applied Food Research, Journal Year: 2024, Volume and Issue: unknown, P. 100658 - 100658

Published: Dec. 1, 2024

Language: Английский

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Ultrasound-assisted solid-state fermentation by Aspergillus niger increased phenolics and antioxidants’ accumulation in Citrus reticulata peels

Food Bioscience, Journal Year: 2024, Volume and Issue: unknown, P. 105699 - 105699

Published: Dec. 1, 2024

Language: Английский

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