Cited by Mitochondrial transfer of drug-loaded artificial mitochondria for enhanced anti-Glioma therapy through synergistic apoptosis/ferroptosis/immunogenic cell death

S100A8-CAMKK2-AMPK axis confers the protective effects of mild hypothermia against cerebral ischemia-reperfusion injury in rats DOI

Dandan Zhang,

Yuting Dai,

Xiaoyan Xu

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Jan. 22, 2025

To investigate the neuroprotective mechanism of mild hypothermia (MH) in ameliorating cerebral ischemia reperfusion (IR) injury. The Pulsinelli's four-vessel ligation method was utilized to establish a rat model global IR role S100A8 MH treatment injury, hippocampus-specific loss or gain function achieved using an adeno-associated virus system. We examined effect over-expression knock-down on SH-SY5Y cell line subjected oxygen-glucose deprivation reoxygenation (OGDR) injury under and delved into underlying mechanisms. significantly ameliorates IR-induced neurological brain. Similarly MH, reduced neuronal oxidative stress, attenuated mitochondrial damage, inhibited apoptosis, improved cognitive rats. Conversely, MH's protective aggravated brain In vitro, low expression decline membrane potential induced by OGDR, stress response, decreased acting as agent nearly equivalent cells. However, these effects MH. Mechanistically, down-regulated expression, enhancing via activation CAMKK2/AMPK signaling pathway. Moreover, with treatment, administration CAMKK2 AMPK inhibitors STO-609 Dorsomorphin increased thereby diminishing against Our study identified master regulator that enables ameliorate during early stage function. By targeting S100A8-initiated pathway, we may unlock novel therapeutic intervention develop refined strategy

Language: Английский

Citations

Nuclear receptor coactive 4-mediated ferritinophagy: a key role of heavy metals toxicity DOI

Wan-Xue Xu,

Wen Xue, Yupeng Fu

et al.

Archives of Toxicology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 10, 2025

Language: Английский

Citations

Ferroptosis: a novel mechanism of cell death in ophthalmic conditions DOI

Yaqi Yang,

Yumeng Lin,

Zhongyu Han

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: June 27, 2024

Ferroptosis, a new type of programmed cell death proposed in recent years, is characterized mainly by reactive oxygen species and iron-mediated lipid peroxidation differs from death, such as apoptosis, necrosis, autophagy. Ferroptosis associated with variety physiological pathophysiological processes. Recent studies have shown that ferroptosis can aggravate or reduce the occurrence development diseases targeting metabolic pathways signaling tumors, ischemic organ damage, other degenerative related to peroxidation. Increasing evidence suggests closely linked onset progression various ophthalmic conditions, including corneal injury, glaucoma, age-related macular degeneration, diabetic retinopathy, retinal detachment, retinoblastoma. Our review current research on reveals significant advancements our understanding pathogenesis, aetiology, treatment these conditions.

Language: Английский

Citations

Structural characteristics and anti-tumor activity of alkali-extracted acidic polysaccharide extracted from Panax ginseng DOI

Keyao Dai, Chao Liu, Haiyu Ji

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: 305, P. 141230 - 141230

Published: Feb. 17, 2025

Language: Английский

Citations

Machine learning-derived diagnostic model of epithelial ovarian cancer based on gut microbiome signatures DOI

Cheng Chen, Chengyuan Deng, Yanwen Li

et al.

Journal of Translational Medicine, Journal Year: 2025, Volume and Issue: 23(1)

Published: March 13, 2025

Prior studies have elucidated that alterations in gut microbiota are associated with a spectrum of tumors and metabolic disorders. However, the diagnostic value epithelial ovarian cancer remains insufficiently investigated. A total 34 patients diagnosis (EOC), 15 benign (TB), 30 healthy volunteers (NOR) were enrolled this study. Fecal samples collected, followed by sequencing V3–V4 region 16S rRNA gene. The clinical data pathological characteristics comprehensively recorded for further analysis, PICRUSt2 was utilized to conduct an analysis microbial functional predictions, WGCNA networks constructed integrating microbiome data. LEfSe employed identify markers, LASSO SVM analyses used screen markers conjunction Cally index, establish Microbial-Cally model. Bootstrap resampling internal validation model, whereas Hosmer–Lemeshow test decision curve (DCA) evaluate performance Plasma subjected untargeted metabolomics profiling, differential key metabolites significantly altered cancer. At same time, Spearman correlation study association between metabolites. supernatants from Escherichia coli Bifidobacterium cultures co-cultured SKOV3 cells. Cell proliferation, migration, invasion evaluated using Counting Kit-8 (CCK-8) assay, Transwell migration assays. Apoptosis assessed flow cytometry fluorescence signals Annexin V propidium iodide (PI) staining. Compared Nor TB populations, individuals diagnosed EOC demonstrated diminished diversity when contrasted both normal controls those presenting conditions. Specifically, relative abundance Bilophila, Bifidobacterium, other probiotics reduced while Shigella marked enrichment within cohort. Differential microorganisms identified through application machine learning techniques delineate characteristic profiles patients. significant index microorganisms. In conclusion, we biomarkers alongside model cancer, receiver operating (ROC) Area Under Curve (AUC) 0.976 (95%CI 0.943–1.00), AUC obtained 0.974. revealed robust concordance observed probabilities predicted generated provided net benefit. 233 group NT (NOR TB) groups. Among these, eight specific (HMDB0243492, C09265, HMDB0242046, HMDB0240606, C04171, HMDB0060557, HMDB0252797, C21412) exclusively derived microbiome. Notably, metabolite HMDB0240606 exhibited positive Shigella, it showed negative Ruminococcus. vitro possessed anti-tumor activity, pro-tumor activity. This provides inaugural comprehensive composition its among tumors, Hunan province, China.

Language: Английский

Citations

Multiple roles of mitochondria in tumorigenesis and treatment: from mechanistic insights to emerging therapeutic strategies DOI

Min Zhang, Yuchen Yang, Jiacheng Wu

et al.

ONCOLOGIE, Journal Year: 2025, Volume and Issue: unknown

Published: March 17, 2025

Language: Английский

Citations

Sodium citrate pretreatment enhances CAR-T cell persistence and anti-tumor efficacy through inhibition of calcium signaling DOI

Xuechen Yin, Wenwen Chen,

Xudong Ao

et al.

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: March 17, 2025

Chimeric antigen receptor T cell (CAR-T) therapy has shown success in treating hematological malignancies, but its effectiveness against solid tumors is hindered by exhaustion. During vitro expansion, tonic signaling induced CAR expression contributes to CAR-T exhaustion, which can be mitigated inhibiting calcium signaling. Given that sodium citrate chelate ions and inhibit signaling, this study, we investigated whether could reduce exhaustion enhance function. We constructed anti-CD70 cells cultured them the presence of citrate. The characteristics functionality citrate-pretreated were assessed through vivo experiments. To further validate our observation, also treated anti-mesothelin (MSLN) with detected phenotypes anti-tumor function cells. found exhibited reduced increased memory proportions, enhanced efficacy both vivo. Notably, treatment improved persistence prevented tumor recurrence. These beneficial effects observed anti-MSLN Transcriptomic metabolite analyses revealed inhibited mTORC1 activity, glycolysis pathways, thus modulating differentiation. Our findings suggest supplementation during expansion a promising strategy improve for preventing promoting formation.

Language: Английский

Citations

Investigating the interaction between calcium signaling and ferroptosis for novel cancer treatment DOI

Haohao Yan,

Ying‐Chuang Zhang,

Yuqing Niu

et al.

Phytomedicine, Journal Year: 2025, Volume and Issue: 137, P. 156377 - 156377

Published: Jan. 6, 2025

Language: Английский

Citations

Dual‐Gating Strategy: Ultrasound Activation of TRPV2 Channels and Borate‐Glass‐Induced Calcium Overload for Tumor Suppression DOI

Hai-Hong Zhu,

Liping Ouyang, Yijie Huang

et al.

Advanced Science, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 27, 2025

Abstract Effective and precise treatment of breast cancer, particularly with bone metastasis, remains a significant challenge. Here, dual‐gating strategy combining locally delivered borate glass (BG) ultrasound (US) is developed for the effective inhibition cancer by targeting transient receptor potential vanilloid 2 (TRPV2). The results demonstrate that after local delivery BG to solid tumor, US effectively triggers calcium overload activating overexpressed TRPV2 channels, leading mitochondrial autophagy apoptosis in cells, thereby inhibiting tumor growth high precision. These effects are validated subcutaneous, orthotopic, TRPV2‐overexpressing mouse models. In metastasis model, combined simultaneously suppresses promotes regeneration. Overall, this offers safe efficient approach cancers expression provides new insights into design clinical translation calcium‐overload‐based therapies.

Language: Английский

Citations

Anticancer effects of high-dose extracellular citrate treatment in pancreatic cancer cells under dzifferent glucose concentrations DOI

Wonjin Kim, Sanghee Park,

Tae-Hyun Park

et al.

Heliyon, Journal Year: 2024, Volume and Issue: 10(18), P. e37917 - e37917

Published: Sept. 1, 2024

Language: Английский

Citations