Microscopic Visualization of Cell-Cell Adhesion Complexes at Micro and Nanoscale DOI Creative Commons
Bieke Vanslembrouck, Jian-Hua Chen, Carolyn A. Larabell

et al.

Frontiers in Cell and Developmental Biology, Journal Year: 2022, Volume and Issue: 10

Published: April 20, 2022

Considerable progress has been made in our knowledge of the morphological and functional varieties anchoring junctions. Cell-cell adhesion contacts consist discrete junctional structures responsible for mechanical coupling cytoskeletons allow transmission signals across cell collective. The three main complexes are adherens junctions, tight desmosomes. Microscopy played a fundamental role understanding these on different levels both physiological pathological conditions. In this review, we discuss light electron microscopy techniques used to unravel structure composition cell-cell epithelial endothelial cells. It functions as guide pick appropriate imaging technique(s) interest. We also point out latest that have emerged. At end, problems investigators encounter during their research using microscopic techniques.

Language: Английский

Location, location, location: Protein kinase nanoclustering for optimised signalling output DOI Creative Commons
Rachel S. Gormal, Ramón Martínez‐Mármol, Andrew J. Brooks

et al.

eLife, Journal Year: 2024, Volume and Issue: 13

Published: Jan. 11, 2024

Protein kinases (PKs) are proteins at the core of cellular signalling and thereby responsible for most physiological processes their regulations. As all intracellular proteins, PKs subjected to Brownian thermal energy that tends homogenise distribution throughout volume cell. To access substrates perform critical functions, PK localisation is therefore tightly regulated in space time, relying upon a range clustering mechanisms. These include post-translational modifications, protein-protein protein-lipid interactions, as well liquid-liquid phase separation, allowing spatial restriction ultimately regulating substrates. In this review, we will focus on key mechanisms mediating nanoclustering pathophysiological processes. We propose nanoclusters act quantal unit output capable integration regulation time. specifically outline various super-resolution microscopy approaches currently used elucidate composition driving nanoscale explore pathological consequences altered kinase context neurodegenerative disorders, inflammation, cancer.

Language: Английский

Citations

10
Probing protein aggregation through spectroscopic insights and multimodal approaches: A comprehensive review for counteracting neurodegenerative disorders DOI Creative Commons
Sania Bashir,

Ayesha Aiman,

Anis Ahmad Chaudhary

et al.

Heliyon, Journal Year: 2024, Volume and Issue: 10(7), P. e27949 - e27949

Published: March 18, 2024

Aberrant accumulation of protein misfolding can cause aggregation and fibrillation is one the primary characteristic features neurodegenerative diseases. Because they are disordered, misfolded, aggregated proteins pose a significant setback in drug designing. The structural study intermediate steps these kinds will allow us to determine conformational changes as well probable pathways encompassing various disorders. analysis aggregates involved diseases relies on diverse toolkit biophysical techniques, both morphological non-morphological methods. Additionally, Thioflavin T (ThT) assays Circular Dichroism (CD) spectroscopy facilitate investigations into kinetics secondary structure alterations. collective application techniques empowers researchers comprehensively unravel intricate nature associated with neurodegeneration. Furthermore, topics covered this review have summed up handful well-established used for aggregation. This multifaceted approach advances our fundamental understanding underlying mechanisms driving informs potential therapeutic strategies.

Language: Английский

Citations

10
Expansion microscopy: A chemical approach for super-resolution microscopy DOI Creative Commons
Yinyin Zhuang, Xiaoyu Shi

Current Opinion in Structural Biology, Journal Year: 2023, Volume and Issue: 81, P. 102614 - 102614

Published: May 28, 2023

Super-resolution microscopy is a series of imaging techniques that bypass the diffraction limit resolution. Since 1990s, optical approaches, such as single-molecular localization microscopy, have allowed us to visualize biological samples from sub-organelle molecular level. Recently, chemical approach called expansion emerged new trend in super-resolution microscopy. It physically enlarges cells and tissues, which leads an increase effective resolution any microscope by length factor. Compared with has lower cost higher depth but requires more complex procedure. The integration advanced microscopes significantly pushed forward boundary This review covers current state art including latest methods their applications, well challenges opportunities for future research.

Language: Английский

Citations

23
Beyond DNA: new probes for PAINT super-resolution microscopy DOI Creative Commons
Marrit M. E. Tholen, Roderick P. Tas, Yuyang Wang

et al.

Chemical Communications, Journal Year: 2023, Volume and Issue: 59(54), P. 8332 - 8342

Published: Jan. 1, 2023

Point accumulation for imaging in nanoscale topography (PAINT) has emerged as a versatile tool single-molecule localization microscopy (SMLM), with broad range of probes.

Language: Английский

Citations

17
Self-inspired learning for denoising live-cell super-resolution microscopy DOI

Liying Qu,

Shiqun Zhao,

Yuanyuan Huang

et al.

Nature Methods, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 11, 2024

Language: Английский

Citations

7
Imaging dendritic cell functions* DOI
Berislav Bošnjak,

Kim Thi Hoang,

Reinhold Förster

et al.

Immunological Reviews, Journal Year: 2021, Volume and Issue: 306(1), P. 137 - 163

Published: Dec. 3, 2021

Abstract Dendritic cells (DCs) are crucial for the appropriate initiation of adaptive immune responses. During inflammation, DCs capture antigens, mature, and migrate to lymphoid tissues present foreign material naïve T cells. These get activated differentiate either into pathogen‐specific cytotoxic CD8 + that destroy infected or CD4 helper that, among other effector functions, orchestrate antibody production by B DC‐mediated antigen presentation is equally important in non‐inflammatory conditions. Here, mediate induction tolerance presenting self‐antigens harmless environmental antigens induce differentiation regulatory inactivation self‐reactive Detailed insights biology are, therefore, development novel vaccines as well prevention autoimmune diseases. As many life science areas, our understanding DC would be extremely restricted without bioimaging, a compilation methods visualize biological processes. Spatiotemporal tracking relies on various imaging tools, which not only enable their positioning migration within entire organs but also allow visualization subcellular molecular This review aims provide an overview toolbox examples diverse techniques used obtain fundamental biology.

Language: Английский

Citations

41
Hello from the other side: Membrane contact of lipid droplets with other organelles and subsequent functional implications DOI

Rebekah Rakotonirina-Ricquebourg,

Vítor Costa, Vítor Teixeira

et al.

Progress in Lipid Research, Journal Year: 2021, Volume and Issue: 85, P. 101141 - 101141

Published: Nov. 16, 2021

Language: Английский

Citations

40
Genetically encodable fluorescent protein markers in advanced optical imaging DOI Creative Commons
Karin Nienhaus, G. Ulrich Nienhaus

Methods and Applications in Fluorescence, Journal Year: 2022, Volume and Issue: 10(4), P. 042002 - 042002

Published: June 29, 2022

Abstract Optical fluorescence microscopy plays a pivotal role in the exploration of biological structure and dynamics, especially on live specimens. Progress field relies, one hand, technical advances imaging data processing and, other progress fluorescent marker technologies. Among these, genetically encodable proteins (FPs) are invaluable tools, as they allow facile labeling cells, tissues or organisms, these produce FP markers all by themselves after introduction suitable gene. Here we cover from GFP family well tetrapyrrole-binding proteins, which further complement toolbox important ways. A broad range variants have been endowed, using protein engineering, with photophysical properties that essential for specific techniques, notably those offering nanoscale image resolution. We briefly introduce various advanced methods show how utilize distinct exciting applications, aim to guide researchers toward design powerful experiments optimally suited address their questions.

Language: Английский

Citations

24
Introduction to Fluorescence Microscopy DOI
Radek Macháň

Springer series on fluorescence, Journal Year: 2022, Volume and Issue: unknown, P. 141 - 200

Published: Jan. 1, 2022

Language: Английский

Citations

23
A hierarchical pathway for assembly of the distal appendages that organize primary cilia DOI Creative Commons
Tomoharu Kanie, Julia F. Love,

Saxton D Fisher

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Jan. 8, 2023

Distal appendages are nine-fold symmetric blade-like structures attached to the distal end of mother centriole. These critical for formation primary cilium, by regulating at least four steps: ciliary vesicle recruitment, recruitment and initiation intraflagellar transport (IFT), removal CP110. While specific proteins that localize have been identified, how exactly each protein functions achieve multiple roles is poorly understood. Here we comprehensively analyze known newly discovered appendage (CEP83, SCLT1, CEP164, TTBK2, FBF1, CEP89, KIZ, ANKRD26, PIDD1, LRRC45, NCS1, C3ORF14) their precise localization, order in step cilia formation. Using CRISPR-Cas9 knockouts, show highly interconnected a more complex hierarchy. Our analysis highlights two modules, CEP83-SCLT1 CEP164-TTBK2, as structural assembly appendages. Functional assay revealed CEP89 selectively RAB34+ while deletion integral components, CEP83-SCLT1-CEP164-TTBK2, severely compromised all steps cilium Collectively, our analyses provide comprehensive view organization function appendage, paving way molecular understanding assembly.

Language: Английский

Citations

16