Generally,
thin-layer
chromatography
always
undertakes
the
indispensable
role
in
rapid
screening
and
identification
of
specific
compounds.
Stationary
phase
is
core
part
with
fixed
property,
which
leading
to
limitations
separation
mode
only
regulating
composition
mobile
phase.
This
work
was
an
attempt
fabricate
unique
photosensitive
make
up
above
major
drawback.
4-[3-(Triethoxysilyl)propoxy]azobenzene
(azo-PTES)
synthesized
as
modifier
stationary
phase,
transformation
cis-trans
structure
azo-PTES
proceeds
along
polarity
difference
under
365
nm
473
irradiation.
Based
on
this,
proposed
shows
reversible
switch
by
photoinduction,
followed
deserved
behavior.
Furthermore,
multi-regulation
spatial
dimension
achieved
based
high
freedom
regulation
brings
about
integration
different
polarity,
just
photoinduction.
The
concept
provides
new
idea
for
improving
efficiency
developing
multi-dimensional
one
plate.
Molecules,
Journal Year:
2023,
Volume and Issue:
28(13), P. 5163 - 5163
Published: July 2, 2023
The
present
review
explores
the
growing
interest
in
techniques
employed
for
extracting
natural
products.
It
emphasizes
limitations
of
conventional
extraction
methods
and
introduces
superior
non-conventional
alternatives,
particularly
ultrasound-assisted
extraction.
Characterization
quantification
bioactive
constituents
through
chromatography
coupled
with
spectroscopy
are
recommended,
while
importance
method
development
validation
biomarker
is
underscored.
At
present,
electrospun
fibers
provide
a
versatile
platform
incorporating
extracts
have
extensive
potential
diverse
fields
due
to
their
unique
structural
functional
characteristics.
Thus,
also
highlights
fabrication
containing
extracts.
preparation
biologically
active
under
optimal
conditions,
including
selection
safe
solvents
cost-effective
equipment,
holds
promising
pharmaceutical,
food,
cosmetic
industries.
Integration
experimental
design
into
procedures
formulation
essential
efficient
production
health
applications
encapsulating
product
fibers,
such
as
wound
healing,
antibacterial
activity,
antioxidant
properties,
acknowledging
need
further
exploration
optimization
this
field.
findings
discussed
anticipated
serve
valuable
resource
processing
industry,
enabling
utilization
affordable
environmentally
friendly,
natural,
raw
materials.
Journal of Chromatography Open,
Journal Year:
2023,
Volume and Issue:
4, P. 100096 - 100096
Published: July 13, 2023
Over
the
past
few
decades,
chemiluminescence
technique
in
analytical
method
development
has
become
an
active
area
of
research.
Antioxidant
analysis
food
matrices
is
a
key
application
due
to
its
high
selectivity
and
sensitivity.
Different
methods
have
been
developed
for
antioxidants
over
years.
However,
chemiluminescence-based
antioxidant
not
covered
as
single
topic
review
form
yet.
The
aim
this
critical
article
provide
reader
with
primary
source
find
information
on
principle,
pros
cons
compared
more
conventional
analysis,
recent
applications,
future
directions,
routine
implementation,
perspectives.
Water Research,
Journal Year:
2024,
Volume and Issue:
265, P. 122169 - 122169
Published: July 29, 2024
The
investigation
of
pollutant
inputs
via
stormwater
runoff
and
subsequent
effects
in
receiving
waters
is
becoming
increasingly
urgent
view
climate
change
with
accompanying
extreme
weather
situations
such
as
heavy
rainfall
events.
In
this
study,
two
sampling
areas,
one
urban
rural
but
dominated
by
a
highway,
were
investigated
using
effect-directed
analysis
to
identify
endocrine
neurotoxic
potentially
responsible
substances
structures
waters.
For
purpose,
transgenic
yeast
cell
assay
for
the
simultaneous
detection
estrogenic,
androgenic,
progestogenic
(YMEES)
was
performed
directly
on
high-performance
thin-layer
chromatography
(HPTLC)
plates.
Concomitantly,
estrogens
analyzed
GC-MS/MS
other
micropollutants
typical
wastewater
LC-MS/MS.
Discharges
from
combined
sewer
overflow
(CSO)
contribute
large
portion
load
studied
water
body,
even
surpassing
nearby
treatment
plant
(WWTP).
An
effect
pattern
similar
CSO
sample
shown
after
lower
intensities,
consisting
an
effect.
contrast,
WWTP,
only
estrogenic
intensity
detected.
Concentrations
E1,
17α-E2,
17β-E2,
EE2,
E3
2000,
410,
1100,
560,
2700
pg/L,
respectively.
HPTLC-YMEES
complement
each
very
well
help
elucidate
stresses.
Acetylcholinesterase
(AChE)
inhibitory
could
not
be
assigned
causative
compound
suspect
non-target
LC-HRMS.
However,
workflow
showed
how
information
HPTLC
separation,
effect-based
methods,
meta-information
area
substance
properties
can
identification
effect-responsible
substances.
Overall,
study
demonstrated
that
methods
combination
instrumental
implemented
investigate
pollution
run-off
particularly
regarding
rain
events
due
change.
Chromatographic
methods
are
widely
used
in
the
separation
and
identification
of
bioactive
compounds
complex
samples.
Chromatography
is
a
technique
that
based
on
components
mixture
into
stationary
mobile
phase.
There
several
types
chromatography,
including
liquid
chromatography
(HPLC),
gas
(GC),
thin-layer
(TLC),
ion-exchange
chromatography.
Each
type
has
its
advantages
disadvantages
more
suitable
for
different
samples
compounds.
For
example,
HPLC
often
to
separate
high-polarity
compounds,
while
GC
best
suited
volatile
can
be
combined
with
other
analytical
methods,
such
as
mass
spectrometry,
help
identify
specific
sample.
Together,
these
chromatographic
spectrometric
have
been
characterization
plants,
microorganisms,
natural
matrices.
