Progressive membrane-binding mechanism of SARS-CoV-2 variant spike proteins

iScience, Journal Year: 2022, Volume and Issue: 25(8), P. 104722 - 104722

Published: July 4, 2022

Membrane recognition by viral spike proteins is critical for infection. Here we show the host cell membrane-binding surfaces of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants Alpha, Beta, Gamma, Delta, Epsilon, Kappa, and Omicron as well SARS-CoV-1 pangolin bat relatives. They increases in membrane binding propensities over time, with all head mutations variants, particularly BA.1, impacting protein's affinity to membranes. Comparison hundreds structures yields a progressive model docking which protein trimers shift from initial perpendicular stances increasingly tilted positions that draw particles alongside membranes before optionally engaging angiotensin-converting enzyme (ACE2) receptors. This culminates assembly symmetric fusion apparatus, enhanced interactions explaining their unique capacities COVID-19 disease transmission rates.

Language: Английский

---

Magnetic biosensors for identification of SARS-CoV-2, Influenza, HIV, and Ebola viruses: a review

Nanotechnology, Journal Year: 2023, Volume and Issue: 34(27), P. 272001 - 272001

Published: March 30, 2023

Abstract Infectious diseases such as novel coronavirus (SARS-CoV-2), Influenza, HIV, Ebola, etc kill many people around the world every year (SARS-CoV-2 in 2019, Ebola 2013, HIV 1980, Influenza 1918). For example, SARS-CoV-2 has plagued higher than 317 000 from December 2019 to January 13, 2022. Some infectious do not yet have a proper vaccine, drug, therapeutic, and/or detection method, which makes rapid identification and definitive treatments main challenges. Different device techniques been used detect diseases. However, recent years, magnetic materials emerged active sensors/biosensors for detecting viral, bacterial, plasmids agents. In this review, applications of biosensors viruses discussed. Also, work addresses future trends perspectives biosensors.

Language: Английский

---

Updates on the Biofunctionalization of Gold Nanoparticles for the Rapid and Sensitive Multiplatform Diagnosis of SARS-CoV-2 Virus and Its Proteins: From Computational Models to Validation in Human Samples

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(11), P. 9249 - 9249

Published: May 25, 2023

Since the outbreak of pandemic respiratory virus SARS-CoV-2 (COVID-19), academic communities and governments/private companies have used several detection techniques based on gold nanoparticles (AuNPs). In this emergency context, colloidal AuNPs are highly valuable easy-to-synthesize biocompatible materials that can be for different functionalization strategies rapid viral immunodiagnosis. review, latest multidisciplinary developments in bioconjugation its proteins (spiked) real samples discussed first time, with reference to optimal parameters provided by three approaches: one theoretical, via computational prediction, two experimental, using dry wet chemistry single/multistep protocols. Overall, achieve high specificity low limits target biomolecules, running buffers bioreagent dilutions nanostructure washes should validated before conducting optical, electrochemical, acoustic biosensing investigations. Indeed, there is plenty room improvement nanomaterials as stable platforms ultrasensitive simultaneous “in vitro” untrained public whole virus, proteins, specific developed IgA/IgM/IgG antibodies (Ab) bodily fluids. Hence, lateral flow assay (LFA) approach a quick judicious solution combating pandemic. author classifies LFAs according four generations guide readers future development multifunctional platforms. Undoubtedly, LFA kit market will continue improve, adapting researchers’ multidetection smartphones easy-to-analyze results, establishing user-friendly tools more effective preventive medical treatments.

Language: Английский

---

Central Countries' and Brazil's Contributions to Nanotechnology

Current Nanomaterials, Journal Year: 2023, Volume and Issue: 9(2), P. 109 - 147

Published: May 26, 2023

Abstract: Nanotechnology is a cornerstone of the scientific advances witnessed over past few years. applications are extensively broad, and an overview main trends worldwide can give insight into most researched areas gaps to be covered. This document presents trend topics three leading countries studying in this area, as well Brazil for comparison. The data mining was made from Scopus database analyzed using VOSviewer Voyant Tools software. More than 44.000 indexed articles published 2010 2020 revealed that responsible highest number United States, China, India, while fifteenth position. Thematic global networks standing-out research health science, energy, wastewater treatment, electronics. In temporal observation, primary are: India (2020), which devoted facing SARS-COV 2; (2019), developing promising strategies combat cancer; China (2018), whit on nanomedicine triboelectric nanogenerators; States (2017) Global tendencies (2018) also related development nanogenerators. collected available GitHub. study demonstrates innovative use data-mining technologies gain comprehensive understanding nanotechnology's contributions highlights diverse priorities nations cutting-edge field.

Language: Английский

---

Commercializing Covid-19 diagnostic technologies: A review of challenges, success factors, and insights from the profiting from innovation framework

Technovation, Journal Year: 2023, Volume and Issue: 130, P. 102903 - 102903

Published: Nov. 18, 2023

Language: Английский

---