Proteomics of urinary small extracellular vesicles in early diagnosis of kidney diseases in children‐expectations and limitations DOI
Klaudia Korecka, Marta Gawin, Agnieszka Pastuszka

et al.

PROTEOMICS, Journal Year: 2024, Volume and Issue: 24(11)

Published: Jan. 11, 2024

Abstract The primary function of the kidneys is to maintain systemic homeostasis (disruption renal structure and results in multilevel impairment body function). Kidney diseases are characterized by a chronic, progressive course may result development chronic kidney disease (CKD). Evaluation composition proteome urinary small extracellular vesicles (sEVs) as so‐called liquid biopsy promising new research direction. Knowing sEV could allow localization cellular changes specific sections nephron or interstitial tissue before fixed changes, detectable only at an advanced stage disease, occur. Research currently underway on role sEVs diagnosis monitoring many entities. Reports literature subject include: diabetic nephropathy, focal glomerulosclerosis glomerulopathies, fibrosis various etiologies. Studies pediatric patients still few, involving piloting if groups without validation studies. Here, we review addressing use for most common disorders children. We evaluate clinical utility define limitations markers present potential biopsy.

Language: Английский

Tracking of Extracellular Vesicles’ Biodistribution: New Methods and Approaches DOI Open Access
А. М. Аймалетдинов, Marina Gomzikova

International Journal of Molecular Sciences, Journal Year: 2022, Volume and Issue: 23(19), P. 11312 - 11312

Published: Sept. 25, 2022

Extracellular vesicles (EVs) are nanosized lipid bilayer that released by almost all cell types. They range in diameter from 30 nm to several micrometres and have the ability carry biologically active molecules such as proteins, lipids, RNA, DNA. EVs natural vectors play an important role many physiological pathological processes. The amount composition of human biological fluids serve biomarkers used for diagnosing diseases monitoring effectiveness treatment. promising use therapeutic agents drug delivery. However, successful clinical practice requires understanding their biodistribution organism. Numerous studies conducted so far on show that, after intravenous administration, mostly localized organs rich blood vessels associated with reticuloendothelial system, liver, lungs, spleen, kidneys. In order improve resolution, new dyes labels being developed detection methods optimized. this work, we review available modern approaches assess EVs, well discuss advantages limitations.

Language: Английский

Citations

50
Extracellular Vesicles as New Players in Drug Delivery: A Focus on Red Blood Cells-Derived EVs DOI Creative Commons
Sara Biagiotti,

Faiza Abbas,

Mariele Montanari

et al.

Pharmaceutics, Journal Year: 2023, Volume and Issue: 15(2), P. 365 - 365

Published: Jan. 21, 2023

The article is divided into several sections, focusing on extracellular vesicles’ (EVs) nature, features, commonly employed methodologies and strategies for their isolation/preparation, characterization/visualization. This work aims to give an overview of advances in EVs’ extensive nanomedical-drug delivery applications. Furthermore, considerations EVs translation clinical application are summarized here, before the review a special kind vesicles, ones derived from red blood cells (RBCEVs). Generally, employing as drug carriers means managing entities with advantageous properties over synthetic vehicles or nanoparticles. Besides fact that certain also reveal intrinsic therapeutic characteristics, regenerative medicine, nanosize, lipidomic proteomic profiles enable them pass biologic barriers display cell/tissue tropisms; indeed, engineering can further optimize organ targeting. In second part review, we focus our attention RBCEVs. First, describe biogenesis composition those naturally produced by (RBCs) under physiological pathological conditions. Afterwards, discuss current procedures isolate and/or produce RBCEVs lab load specific cargo exploitation. Finally, disclose most recent applications at vitro preclinical research level potential industrial conclusion, be, near future, very promising versatile platform pharmaceutical exploitations.

Language: Английский

Citations

27
Extracellular vesicle biopotentiated hydrogels for diabetic wound healing: The art of living nanomaterials combined with soft scaffolds DOI Creative Commons
Zhenzhen Yan, Tinglin Zhang, Yuxiang Wang

et al.

Materials Today Bio, Journal Year: 2023, Volume and Issue: 23, P. 100810 - 100810

Published: Sept. 22, 2023

Diabetic wounds (DWs) pose a major challenge for the public health system owing to their high incidence, complex pathogenesis, and long recovery time; thus, there is an urgent need develop innovative therapies accelerate healing process of diabetic wounds. As natural nanovesicles, extracellular vesicles (EVs) are rich in sources with low immunogenicity abundant nutritive molecules exert potent therapeutic effects on wound healing. To avoid rapid removal EVs, suitable delivery required controlled release. Owing advantages porosity, good biocompatibility, adjustable physical chemical properties hydrogels, EV biopotentiated hydrogels can aid achieving precise favorable therapy against This review highlights different design strategies, effects, mechanisms hydrogels. We also discussed future challenges opportunities using

Language: Английский

Citations

23
Emerging extracellular vesicle-based carriers for glioblastoma diagnosis and therapy DOI
Jingjing Wang, Yue Liu, Fengbo Liu

et al.

Nanoscale, Journal Year: 2023, Volume and Issue: 15(26), P. 10904 - 10938

Published: Jan. 1, 2023

This review summarizes recent advances in extracellular vesicle-based carriers for glioblastoma diagnosis and therapy, points out the current challenges perspectives.

Language: Английский

Citations

18
Application of nanotechnology in the treatment of glomerulonephritis: current status and future perspectives DOI Creative Commons

He-Qin Zhan,

Xiaoxun Zhang, Xu‐Lin Chen

et al.

Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)

Published: Jan. 3, 2024

Abstract Glomerulonephritis (GN) is the most common cause of end-stage renal failure worldwide; in cases, it cannot be cured and can only delay progression disease. At present, main treatment methods include symptomatic therapy, immunosuppressive replacement therapy. However, effective GN hindered by issues such as steroid resistance, serious side effects, low bioavailability, lack precise targeting. With widespread application nanoparticles medical treatment, novel have emerged for kidney diseases. Targeted transportation drugs, nucleic acids, other substances to tissues even cells through nanodrug delivery systems reduce systemic effects adverse reactions drugs improve effectiveness. The high specificity enables them bind ion channels block or enhance channel gating, thus improving inflammation. This review briefly introduces characteristics GN, describes status systematically summarizes research achievements primary diabetic nephropathy lupus nephritis, analyzes recent therapeutic developments, outlines promising directions, gas signaling molecule ultrasmall nanoparticles. current summarized provide a reference better future. Graphical

Language: Английский

Citations

9
Engineered extracellular vesicle-encapsulated CHIP as novel nanotherapeutics for treatment of renal fibrosis DOI Creative Commons
Cheng Ji, Jiahui Zhang,

Linru Shi

et al.

npj Regenerative Medicine, Journal Year: 2024, Volume and Issue: 9(1)

Published: Jan. 13, 2024

Abstract Renal interstitial fibrosis (RIF) is a fundamental pathological feature of chronic kidney disease (CKD). However, toxicity and poor renal enrichment inhibitors limit their further applications. In this study, platform for CKD therapy developed using superparamagnetic iron oxide nanoparticles (SPION) decorated mesenchymal stem cells derived extracellular vesicles with carboxyl terminus Hsc70-interacting protein (CHIP) high expression (SPION-EVs) to achieve higher renal-targeting antifibrotic therapeutic effect. SPION-EVs selectively accumulate at the injury sites under an external magnetic field. Moreover, deliver CHIP induce Smad2/3 degradation in tubular which alleviates activation-mediated fibrosis-like changes collagen deposition. The vesicle engineering technology provides potential nanoplatform RIF through CHIP-mediated degradation.

Language: Английский

Citations

9
The Role of Extracellular Vesicles in Aging and Age-Related Disorders DOI Creative Commons

Bharathi Hassan Ganesh,

Himabindu Padinjarathil, Ramya Lakshmi Rajendran

et al.

Antioxidants, Journal Year: 2025, Volume and Issue: 14(2), P. 177 - 177

Published: Feb. 3, 2025

A variety of molecular and cellular changes distinguish the multifaceted biological process aging. Recent studies in this decade have demonstrated essential role extracellular vesicles (EVs) aging process. Mitochondrial malfunction increased oxidative stress are major contributors for This review investigates EVs intercellular communication, tissue regeneration, inflammation context We also discuss exosome its utility to reduce stress, which is a key part aging, as well possibility using exosomes anti-aging drugs. Changes cargo composition can influence phenotype impact functionality cells tissues. Additionally, during addresses potential treatment strategies development biomarkers age-associated disorders. The highlighted providing antioxidant properties, help excessive reactive oxygen species (ROS) strengthen defenses against stress. it emphasized age-related pathologies, such neurodegenerative diseases, cardiovascular disorders, immunosenescence, offering insights into targeted interventions promoting healthy article provides comprehensive analysis current body knowledge regarding therapeutic effects on with particular emphasis implications emerging field research relationship

Language: Английский

Citations

1
An update on renal fibrosis: from mechanisms to therapeutic strategies with a focus on extracellular vesicles DOI Creative Commons
Cui Wang, Shangwei Li, Xin Zhong

et al.

Kidney Research and Clinical Practice, Journal Year: 2023, Volume and Issue: 42(2), P. 174 - 187

Published: March 31, 2023

The increasing prevalence of chronic kidney disease (CKD) is a major global public health concern. Despite the complicated pathogenesis CKD, renal fibrosis represents most common pathological condition, comprised progressive accumulation extracellular matrix in diseased kidney. Over last several decades, tremendous progress understanding mechanism has been achieved, and corresponding potential therapeutic strategies targeting fibrosis-related signaling pathways are emerging. Importantly, vesicles (EVs) contribute significantly to inflammation by mediating cellular communication. Increasing evidence suggests EV-based therapy fibrosis, which may represent future direction for CKD therapy.

Language: Английский

Citations

16
Decellularized tendon scaffolds loaded with collagen targeted extracellular vesicles from tendon-derived stem cells facilitate tendon regeneration DOI
Jing Cui, Yanjing Zhang, Xuan Li

et al.

Journal of Controlled Release, Journal Year: 2023, Volume and Issue: 360, P. 842 - 857

Published: July 25, 2023

Language: Английский

Citations

13
Mincle receptor in macrophage and neutrophil contributes to the unresolved inflammation during the transition from acute kidney injury to chronic kidney disease DOI Creative Commons
Cui Wang, Yilin Zhang,

Anran Shen

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: May 17, 2024

Recent studies have demonstrated a strong association between acute kidney injury (AKI) and chronic disease (CKD), while the unresolved inflammation is believed to be driving force for this transition process. As transmembrane pattern recognition receptor, Mincle (macrophage-inducible C-type lectin, Clec4e) was identified participate in early immune response after AKI. However, impact of on AKI remains largely unclear. We performed single-cell RNA sequencing (scRNA-seq) with unilateral ischemia-reperfusion (UIR) murine model at days 1, 3, 14 28 injury. Potential effects mechanism renal fibrosis were further validated vivo utilizing knockout mice. The dynamic expression macrophages neutrophils throughout from CKD observed. For both cell types, significantly up-regulated day 1 following AKI, second rise observed 14. Notably, we distinct subclusters Minclehigh that exhibited time-dependent influx dual peaks characterized remarkable pro-inflammatory pro-fibrotic functions. Moreover, represented an "aged" mature neutrophil subset derived "fresh" cluster kidney. Additionally, synergistic whereby Mincle-expressing sustained by tumor necrosis factor (TNF) production. Mincle-deficient mice reduced present findings unveiled combined persistence during AKI-to-CKD transition, contributing followed via TNF-α as central cytokine. Targeting may offer novel therapeutic strategy preventing CKD.

Language: Английский

Citations

5