Mesenchymal Stem Cells‐Derived Small Extracellular Vesicles and Their Validation as a Promising Treatment for Chondrosarcoma in a 3D Model in Vitro DOI Creative Commons
Eugenia Romano, Francesca Perut, Sofia Avnet

et al.

Biotechnology and Bioengineering, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 17, 2024

ABSTRACT Chondrosarcomas (CHS) constitute approximately 20% of all primary malignant bone tumors, characterized by a slow growth rate with initial manifestation few signs and symptoms. These cartilaginous neoplasms, particularly those dedifferentiated histological subtypes, pose significant therapeutic challenges, as they exhibit high resistance to both radiation chemotherapy. Ranging from relatively benign, low‐grade tumors (grade I) aggressive high‐grade the potential for lung metastases grim prognosis, there is critical need innovative diagnostic approaches, patients more forms. Herein, small extracellular vesicles (sEVs) derived mesenchymal stem cells are presented an efficient nanodelivery tool enhance drug penetration in vitro 3D model CHS. Employing high‐pressure homogenization (HPH), we achieved unprecedented encapsulation efficiency doxorubicin (DXR) sEVs (MSC‐EVs). Subsequently, comparative analysis between free DXR MSC‐EVs encapsulated (DXR‐MSC‐EVs) was conducted assess their uptake efficacy model. The results unveiled higher incidence necrotic pronounced toxic effect DXR‐MSC‐EVs compared alone. This underscores remarkable ability deliver drugs complex environments, highlighting application treatment

Language: Английский

Biomimetic Scaffolds—A Novel Approach to Three Dimensional Cell Culture Techniques for Potential Implementation in Tissue Engineering DOI Creative Commons
Tomasz Górnicki, Jakub Lambrinow, Afsaneh Golkar‐Narenji

et al.

Nanomaterials, Journal Year: 2024, Volume and Issue: 14(6), P. 531 - 531

Published: March 16, 2024

Biomimetic scaffolds imitate native tissue and can take a multidimensional form. They are biocompatible influence cellular metabolism, making them attractive bioengineering platforms. The use of biomimetic adds complexity to traditional cell cultivation methods. most commonly used technique involves cultivating cells on flat surface in two-dimensional format due its simplicity. A three-dimensional (3D) provide microenvironment for surrounding cells. There two main techniques obtaining 3D structures based the presence scaffolding. Scaffold-free consist spheroid technologies. Meanwhile, scaffold contain organoids all constructs that various types scaffolds, ranging from decellularized extracellular matrix (dECM) through hydrogels one extensively studied forms potential culture up 4D bioprinted biomaterials. bioprinting is important create scaffolds. versatility this allows many different inks, mainly hydrogels, as well inorganic substances. Increasing amounts data evidence vast usage engineering personalized medicine, with area application being regeneration skin musculoskeletal systems. Recent papers also indicate increasing vivo tests products which further strengthen importance branch emphasize need extensive research safe humansbiomimetic tissues organs. In review article, we recent advancements field preceded by an overview technologies led development complex type culture.

Language: Английский

Citations

22
Multifunctional hydrogel-based engineered extracellular vesicles delivery for complicated wound healing DOI Creative Commons
Zuhao Li,

Jinlong Liu,

Jian Song

et al.

Theranostics, Journal Year: 2024, Volume and Issue: 14(11), P. 4198 - 4217

Published: Jan. 1, 2024

The utilization of extracellular vesicles (EVs) in wound healing has been well-documented.However, the direct administration free EVs via subcutaneous injection at sites may result rapid dissipation bioactive components and diminished therapeutic efficacy.Functionalized hydrogels provide effective protection, as well ensure sustained release bioactivity during process, making them an ideal candidate material for delivering EVs.In this review, we introduce mechanisms by which accelerate healing, then elaborate on construction strategies engineered EVs.Subsequently, discuss synthesis application delivery systems to enhance complicated healing.Furthermore, face wounds, functionalized with specific microenvironment regulation capabilities, such antimicrobial, anti-inflammatory, immune regulation, used loading EVs, potential approaches addressing these challenges.Ultimately, deliberate future trajectories outlooks, offering a fresh viewpoint advancement artificial intelligence (AI)-energized materials 3D bio-printed multifunctional hydrogel-based dressings biomedical applications.

Language: Английский

Citations

15
Hydrogels loaded with MSC‐derived small extracellular vesicles: A novel cell‐free tissue engineering system for diabetic wound management DOI Creative Commons
Weicheng Zhong,

Hao Meng,

Liqian Ma

et al.

View, Journal Year: 2024, Volume and Issue: 5(4)

Published: July 18, 2024

Abstract With the aging and obesity era, increasing incidence of diabetes diabetic complications, especially non‐healing wounds, imposes a serious economic burden on both patients society. The complex microenvironments, including hyperglycemia, bacterial infection, ischemia, nerve damage, lead to prolonged inflammation proliferation phase wounds. Mesenchymal stem cell‐derived small extracellular vesicles (MSC‐sEVs), which contain rich variety therapeutic molecules, have been chased for decades because their potential roles in cellular communication, tissue regeneration, drug delivery. As powerful tools controlled‐sustained release sEVs, biocompatible hydrogels applied wide range biomedical applications. Herein, we first summarize pathological features such as angiopathy, neuropathy, immune cell dysfunction. Then, discuss biological properties, performance, stability pure MSC‐sEVs. After that, components, application patterns, responsiveness hydrogels. Next, loading avenues MSC‐sEVs into hydrogel, behaviors sEVs from hydrogels, influence crosslinking method hydrogel‐sEV composites. Finally, provide an overview current applications loaded with novel cell‐free engineering system managing wounds propose critical unsolved issues. This review is expected meaningful guidance developing wound management.

Language: Английский

Citations

10
Multifunctional type lll recombinant human collagen incorporated sodium alginate hydrogel with sustained release of extra cellular vehicles for wound healing multimodal therapy in diabetic mice DOI Creative Commons
Jia Yao,

Yaxi Han,

Yue Zhang

et al.

Regenerative Therapy, Journal Year: 2024, Volume and Issue: 27, P. 329 - 341

Published: April 16, 2024

The effective promotion of wound healing poses a substantial challenge for clinical treatment. Despite evidence supporting the role extracellular vesicles (EVs) in this process, their therapeutic potential is currently restrict by challenges targeting and maintaining them. manufacturing process rhCol III, or recombinant human collagen stable, rejection rate low. We used cross-linking method to prepare III incorporated sodium alginate (SA) hydrogel, which enabled accomplish an EV sustained release that was site-specific. Cell viability through MTT assay, proliferation ROS generation were performed with MC3T3-E1cell lines. In addition, diabetic wounds are characterised environment hyper-inflammation elevated oxidative stress. III/SA-EVs delivery vehicle anti-inflammatory antioxidant characteristics, promotes setting.

Language: Английский

Citations

9
Immunomodulation effects of collagen hydrogel encapsulating extracellular vesicles derived from calcium silicate stimulated-adipose mesenchymal stem cells for diabetic healing DOI Creative Commons

Yen-Hong Lin,

Yeh Chen,

En‐Wei Liu

et al.

Journal of Nanobiotechnology, Journal Year: 2025, Volume and Issue: 23(1)

Published: Jan. 27, 2025

Diabetic wounds are characterized by chronic inflammation, reduced angiogenesis, and insufficient collagen deposition, leading to impaired healing. Extracellular vesicles (EVs) derived from adipose-derived mesenchymal stem cells (ADSC) offer a promising cell-free therapeutic strategy, yet their efficacy immunomodulation can be enhanced through bioactivation. In this study, we developed calcium silicate (CS)-stimulated ADSC-derived EVs (CSEV) incorporated into hydrogels create sustained-release system for promoting diabetic wound CSEV exhibited protein content, surface marker expression, bioactive cargo enriched with pro-angiogenic anti-inflammatory factors. vitro, CSEV-loaded significantly reactive oxygen species production, promoted cell proliferation migration compared standard EV-loaded collagen. Cytokine profiling revealed the upregulation of cytokines extracellular matrix components, highlighting immunomodulatory regenerative potential. vivo, histological evaluation rabbit models treated superior reepithelialization organized indicating accelerated closure. These findings underscore potential as an innovative effective platform enhancing healing simultaneously addressing inflammation tissue regeneration.

Language: Английский

Citations

1
Optimizing Wound Healing: Examining the Influence of Biopolymers Through a Comprehensive Review of Nanohydrogel-Embedded Nanoparticles in Advancing Regenerative Medicine DOI
M Ahmadi,

Mahdi Sabzini,

Shima Rastgordani

et al.

The International Journal of Lower Extremity Wounds, Journal Year: 2024, Volume and Issue: unknown

Published: April 15, 2024

Nanohydrogel wound healing refers to the use of nanotechnology-based hydrogel materials promote wounds. Hydrogel dressings are made up a three-dimensional network hydrophilic polymers that can absorb and retain large amounts water or other fluids. Nanohydrogels take this concept further by incorporating nanoscale particles structures into matrix. These nanoparticles be various materials, such as silver, zinc oxide, derived from natural substances like chitosan. The inclusion provide additional properties benefits dressings. designed release bioactive substances, growth factors drugs, in controlled manner. This allows for targeted delivery therapeutics site, promoting reducing inflammation. Nanoparticles reinforce structure hydrogels, improving their mechanical strength stability. often incorporate antimicrobial nanoparticles, silver oxide. have shown effective activity against wide range bacteria, fungi, pathogens. By them dressings, nanohydrogels help prevent reduce risk infection encapsulate factors, peptides, sustained therapeutic agents promotes facilitating cell proliferation, inflammation, supporting tissue regeneration. unique nanohydrogels, including ability maintain moist environment deliver agents, accelerate process. creating an optimal repair, faster more efficient

Language: Английский

Citations

8
Advances in the development of medical dressings for the treatment of diabetic foot wounds DOI
Ming Cai, Zhao Liu,

Xun Sun

et al.

Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: 498, P. 155575 - 155575

Published: Sept. 7, 2024

Language: Английский

Citations

6
The influence of the pectin structure on the properties of hydrogel dressings doped with octenidine-containing antiseptic DOI
Marta Fiedot, Adam Junka, Malwina Brożyna

et al.

Carbohydrate Polymers, Journal Year: 2024, Volume and Issue: 343, P. 122463 - 122463

Published: July 4, 2024

Language: Английский

Citations

4
3D printed therapeutic scaffolds for wound healing applications DOI
Sofía Municoy, Pablo Edmundo Antezana,

Sofía Genovés

et al.

Elsevier eBooks, Journal Year: 2025, Volume and Issue: unknown, P. 207 - 226

Published: Jan. 1, 2025

Language: Английский

Citations

0
Extracellular vesicles in burn injury: roles, mechanisms, and applications DOI Creative Commons
Min Wang,

Xinyu Zhao,

Yuyu Cui

et al.

Burns & Trauma, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 23, 2025

Abstract Burn injuries are associated with high morbidity and mortality, severe burns trigger many pathophysiological reactions, such as metabolic changes, distributive shock, inflammatory responses, which potentially devastating to patients. wound management necessitates infection prevention, anti-inflammation, pain management, growth factor but significant obstacles remain. Extracellular vesicles (EVs) lipid bilayer secreted by various cell types, including mammalian cells, plant even prokaryotes, They widely involved in biological processes, survival, neovascularization, immunomodulation. EVs abundant components that can play a role different stages of repair at subcellular levels simultaneously transporting active contents, proteins nucleic acids. Moreover, detectable biofluids burn injury patients thus regarded novel biomarkers for monitoring therapeutic response predicting prognosis. This review summarizes the roles their mechanisms action summarized. The prospects opportunities clinical application wounds also discussed. will stimulate guide additional in-depth studies repair, provide new therapy wounds, reference guidance applying repair.

Language: Английский

Citations

0