Dual‐Crosslinked Bioactive Hydrogel Scaffold for Accelerated Repair of Genital Tract Defect DOI
Liyang Wang,

Leong Chi Cheng,

Yu Chen

et al.

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 5, 2024

Abstract Reproductive health concerns like Mayer‐Rokitansky‐Küster‐Hauser (MRKH) syndrome are prevalent in today's society. MRKH is a condition that severely affects women's sexual life, fertility, and mental has high prevalence of one out 5000 female births. Vaginoplasty the primary method to regain patients’ reproductive health. However, conventional vaginoplasty faces various challenges, including complex non‐customized treatment procedures causing intense pains complications. To bring new advances vaginoplasty, 3D‐printed hydrogel scaffold developed provide satisfactory mechanical support bioactivity for accelerating defect repair after surgery. The consisting gelatin methacryloyl (gelMA) carrageenan (Car) custom using an ambient temperature printing system. Furthermore, undergoes dual‐crosslinking through chemical crosslinking gelMA ionic Car with magnesium ions (Mg 2+ ). This strategy substantially improves overall properties introduces bioactive Mg . sustained release plus extracts from dual‐crosslinked significantly promotes cell proliferation, migration angiogenesis. In preclinical rat model penetrating genital tract defects mimicking implantation repairs wounds near‐normal levels within week, showing potential as alternative better regaining

Language: Английский

A biomimetic piezoelectric scaffold with sustained Mg2+ release promotes neurogenic and angiogenic differentiation for enhanced bone regeneration DOI Creative Commons
Liangyu Wang,

Yanyun Pang,

Yujing Tang

et al.

Bioactive Materials, Journal Year: 2022, Volume and Issue: 25, P. 399 - 414

Published: Nov. 29, 2022

Natural bone is a composite tissue made of organic and inorganic components, showing piezoelectricity. Whitlockite (WH), which natural magnesium-containing calcium phosphate, has attracted great attention in formation recently due to its unique piezoelectric property after sintering treatment sustained release magnesium ion (Mg2+). Herein, scaffold (denoted as PWH scaffold) composed WH (PWH) poly(ε-caprolactone) (PCL) was 3D printed meet the physiological demands for regeneration neuro-vascularized tissue, namely, providing endogenous electric field at defect site. The Mg2+ from scaffold, displaying multiple biological activities, thus exhibits strong synergistic effect with piezoelectricity on inhibiting osteoclast activation, promoting neurogenic, angiogenic, osteogenic differentiation marrow mesenchymal stromal cells (BMSCs) vitro. In rat calvarial model, this remarkably conducive efficient neo-bone rich neurogenic angiogenic expressions. Overall, study presents first example biomimetic vivo, offers new insights regenerative medicine.

Language: Английский

Citations

76
Magnesium Gradient‐Based Hierarchical Scaffold for Dual‐Lineage Regeneration of Osteochondral Defect DOI
Chenyuan Gao, Wenli Dai, Xinyu Wang

et al.

Advanced Functional Materials, Journal Year: 2023, Volume and Issue: 33(43)

Published: July 2, 2023

Abstract Osteochondral regeneration remains a great challenge due to the limited self‐healing ability and complexity of its hierarchical structure composition. Mg 2+ hypoxia are two effective modulators in boosting chondrogenesis. To this end, double‐layered scaffold (D) consisting hydrogel layer on porous cryogel is fabricated mimic osteochondral tissue. An gradient incorporated into with hypoxia‐mimicking deferoxamine (DFO) embedded (D‐Mg‐DFO), which remarkably augments dual‐lineage both cartilage subchondral bone. The higher supplementation from upper hydrogel, associated situation small pore size, exhibits promotive effects chondrogenic differentiation. lower bottom cryogel, interconnected macroporous structure, achieves multiple contributions stem cell migration bone marrow cavity, matrix mineralization, osteogenesis. Furthermore, rabbits’ trochlea defects established evaluate regenerative outcome. Compared control scaffolds containing only or DFO, D‐Mg‐DFO presents best effect under synergistic contribution factors. Overall, work provides new design toward an repair defect.

Language: Английский

Citations

65
An injectable magnesium-loaded hydrogel releases hydrogen to promote osteoporotic bone repair via ROS scavenging and immunomodulation DOI Creative Commons
Hang Zhou,

Zhongyuan He,

Youde Cao

et al.

Theranostics, Journal Year: 2024, Volume and Issue: 14(9), P. 3739 - 3759

Published: Jan. 1, 2024

The repair of osteoporotic bone defects remains challenging due to excessive reactive oxygen species (ROS), persistent inflammation, and an imbalance between osteogenesis osteoclastogenesis.

Language: Английский

Citations

22
3D Printed Multifunctional Biomimetic Bone Scaffold Combined with TP‐Mg Nanoparticles for the Infectious Bone Defects Repair DOI Creative Commons
Xulin Hu, Jiao Chen,

Shuhao Yang

et al.

Small, Journal Year: 2024, Volume and Issue: unknown

Published: May 28, 2024

Abstract Infected bone defects are one of the most challenging problems in treatment due to high antibiotic failure rate and lack ideal grafts. In this paper, inspired by clinical cement filling treatment, α ‐c phosphate ( ‐TCP) with self‐curing properties is composited β ‐tricalcium constructed a bionic cancellous scaffolding system α/β‐tricalcium / low‐temperature 3D printing, gelatin preserved inside scaffolds as an organic phase, later loaded metal–polyphenol network structure tea polyphenol‐magnesium (TP‐Mg) nanoparticles. The mimic components mechanical strength (>100 MPa) based on ‐TCP through printing. Meanwhile, TP‐Mg exhibit significant inhibition Staphylococcus aureus S.aureus ) promote transition macrophages from M1 pro‐inflammatory M2 anti‐inflammatory phenotype. addition, composite scaffold also exhibits excellent bone‐enhancing effects synergistic effect Mg 2+ Ca . study, multifunctional ceramic ‐TCP@TP‐Mg) that integrates anti‐inflammatory, antibacterial, osteoinduction constructed, which promotes late regenerative healing while modulating early microenvironment infected defects, has promising application defects.

Language: Английский

Citations

20
Strategies for promoting neurovascularization in bone regeneration DOI Creative Commons
Xinling Li, Yuqing Zhao, Miao Li

et al.

Military Medical Research, Journal Year: 2025, Volume and Issue: 12(1)

Published: March 3, 2025

Abstract Bone tissue relies on the intricate interplay between blood vessels and nerve fibers, both are essential for many physiological pathological processes of skeletal system. Blood provide necessary oxygen nutrients to bone tissues, remove metabolic waste. Concomitantly, fibers precede during growth, promote vascularization, influence cells by secreting neurotransmitters stimulate osteogenesis. Despite critical roles components, current biomaterials generally focus enhancing intraosseous vessel repair, while often neglecting contribution nerves. Understanding distribution main functions in is crucial developing effective engineering. This review first explores anatomy highlighting their vital embryonic development, metabolism, repair. It covers innovative regeneration strategies directed at accelerating intrabony neurovascular system over past 10 years. The issues covered included material properties (stiffness, surface topography, pore structures, conductivity, piezoelectricity) acellular biological factors [neurotrophins, peptides, ribonucleic acids (RNAs), inorganic ions, exosomes]. Major challenges encountered neurovascularized materials clinical translation have also been highlighted. Furthermore, discusses future research directions potential developments aimed producing repair that more accurately mimic natural healing tissue. will serve as a valuable reference researchers clinicians novel into practice. By bridging gap experimental practical application, these advancements transform treatment defects significantly improve quality life patients with bone-related conditions.

Language: Английский

Citations

3
3D-printed dual-ion chronological release functional platform reconstructs neuro-vascularization network for critical-sized bone defect regeneration DOI

Yuhao Xia,

Xirui Jing, Xiaopei Wu

et al.

Chemical Engineering Journal, Journal Year: 2023, Volume and Issue: 465, P. 143015 - 143015

Published: April 19, 2023

Language: Английский

Citations

31
Organic–inorganic composite hydrogels: compositions, properties, and applications in regenerative medicine DOI
Xinyu Wang, Wei Wei, Ziyi Guo

et al.

Biomaterials Science, Journal Year: 2024, Volume and Issue: 12(5), P. 1079 - 1114

Published: Jan. 1, 2024

Hydrogels, formed from crosslinked hydrophilic macromolecules, provide a three-dimensional microenvironment that mimics the extracellular matrix. They served as scaffold materials in regenerative medicine with an ever-growing demand. However, hydrogels composed of only organic components may not fully meet performance and functionalization requirements for various tissue defects. Composite hydrogels, containing inorganic components, have attracted tremendous attention due to their unique compositions properties. Rigid particles, rods, fibers,

Language: Английский

Citations

18
Ion‐Engineered Microcryogels via Osteogenesis‐Angiogenesis Coupling and Inflammation Reversing Augment Vascularized Bone Regeneration DOI
Yue Wang, Xinyu Wang,

Yanyun Pang

et al.

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: 34(34)

Published: March 10, 2024

Abstract Native bone inherently requires a balanced ionic microenvironment to maintain homeostasis. Hence, scaffolds designed for the sustained release of therapeutic ions into defects hold great promise regeneration. Magnesium (Mg) and silicon (Si) are essential elements, which play crucial roles in process regeneration, impacting immunomodulation, angiogenesis, osteogenesis. Herein, porous cryogel‐type organic–inorganic composite microspheres developed as injectable microscaffolds (denoted GMN). GMN enables Mg/Si at an optimized ratio, achieving most significant synergistic effect on vascularized Various conditioned media obtained explore angiogenesis‐osteogenesis coupling, well crosstalk between marrow mesenchymal stromal cells (BMSCs) macrophages. Meanwhile, autocrine paracrine effects simultaneously modulating functions determining cell fates under guidance biofactors secreted by cells. Overall, ion‐engineering create conducive efficiently augment regeneration tissue vivo, offering versatile platform engineering.

Language: Английский

Citations

17
Dynamic hydrogel–metal–organic framework system promotes bone regeneration in periodontitis through controlled drug delivery DOI Creative Commons

Qipei Luo,

Yuxin Yang,

Chingchun Ho

et al.

Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)

Published: May 26, 2024

Abstract Periodontitis is a prevalent chronic inflammatory disease, which leads to gradual degradation of alveolar bone. The challenges persist in achieving effective bone repair due the unique bacterial microenvironment’s impact on immune responses. This study explores novel approach utilizing Metal–Organic Frameworks (MOFs) (comprising magnesium and gallic acid) for promoting regeneration periodontitis, focuses physiological roles ions acid's antioxidant immunomodulatory properties. However, dynamic oral environment irregular periodontal pockets pose sustained drug delivery. A smart responsive hydrogel system, integrating Carboxymethyl Chitosan (CMCS), Dextran (DEX) 4-formylphenylboronic acid (4-FPBA) was designed address this problem. injectable self-healing forms dual-crosslinked network, incorporating MOF rendering its on-demand release sensitive reactive oxygen species (ROS) levels pH periodontitis. We seek analyze hydrogel’s synergistic effects with MOFs antibacterial functions, immunomodulation promotion In vivo vitro experiment validated system's efficacy inhibiting inflammation-related genes proteins expression foster regeneration. system MOFs, shows promise as potential therapeutic avenue addressing Graphical

Language: Английский

Citations

17
Effects of MgO nanoparticle addition on the mechanical properties, degradation properties, antibacterial properties and in vitro and in vivo biological properties of 3D-printed Zn scaffolds DOI Creative Commons

Leiting Yu,

Fengdong Sun,

Yuanyuan Wang

et al.

Bioactive Materials, Journal Year: 2024, Volume and Issue: 37, P. 72 - 85

Published: March 16, 2024

Bone tissue engineering is the main method for repairing large segment bone defects. In this study, a layer of bioactive MgO nanoparticles was wrapped on surface spherical Zn powders, which allowed to be incorporated into 3D-printed matrix and improved biodegradation biocompatibility matrix. The results showed that porous pure scaffolds Zn/MgO with skeletal-gyroid (G) model structure were successfully prepared by selective laser melting (SLM). average porosity two 59.3 60.0%, respectively. pores uniformly distributed an pore size 558.6–569.3 μm. regulated corrosion rate scaffolds, resulting in more uniform degradation behavior simulated body fluid solution. ratio composite vivo increased compared reaching 15.6% at 12 weeks. yield strength (10.8 ± 2.4 MPa) scaffold comparable cancellous bone, antimicrobial higher than 99%. could better guide regeneration rat cranial repair experiments (completely filling weeks). Therefore, G-model SLM are promising biodegradable scaffold.

Language: Английский

Citations

13