Journal of Ethnopharmacology, Journal Year: 2024, Volume and Issue: 337, P. 118963 - 118963
Published: Oct. 25, 2024
Language: Английский
Burns & Trauma, Journal Year: 2024, Volume and Issue: 12
Published: Jan. 1, 2024
Abstract Extracellular vesicles (EVs) are heterogeneous membrane-like secreted by living cells that involved in many physiological and pathological processes act as intermediaries of intercellular communication molecular transfer. Recent studies have shown EVs from specific sources regulate tissue repair regeneration delivering proteins, lipids, nucleic acids to target signaling molecules. Nanotechnology breakthroughs facilitated the development exploration engineered for repair. Enhancements through gene editing, surface modification, content modification further improved their therapeutic efficacy. This review summarizes potential regeneration, mechanisms action, research progress regenerative medicine. highlights design logic typical examples explores prospects The aim this is provide new insights into applications, thereby expanding use
Language: Английский
Citations
10
Journal of Drug Delivery Science and Technology, Journal Year: 2025, Volume and Issue: 106, P. 106696 - 106696
Published: Feb. 6, 2025
Language: Английский
Citations
1
Neural Regeneration Research, Journal Year: 2024, Volume and Issue: 20(4), P. 1207 - 1216
Published: April 3, 2024
Schwann cells are essential for the maintenance and function of motor neurons, axonal networks, neuromuscular junction. In amyotrophic lateral sclerosis, where neuron is progressively lost, cell may also be impaired. Recently, important signaling potential trophic activities cell-derived exosomal vesicles have been reported. This case report describes treatment a patient with advanced sclerosis using serial intravenous infusions allogeneic vesicles, marking, to our knowledge, first instance such treatment. An 81-year-old male presented 1.5-year history rapidly progressive sclerosis. After initial diagnosis, underwent combination generic riluzole, sodium phenylbutyrate taurursodiol. The volunteered participate in an FDA-approved single-patient expanded access received weekly potentially restore impaired function. We confirmed that cultured obtained from via sural nerve biopsy appeared (senescent) exposure patient’s cadaver donor improved their growth capacity vitro. period observation lasting 10 weeks, during which Functional Rating Scale-Revised pulmonary were regularly monitored, consecutive 1.54 × 12 (×2), then 7.5 (×6) diluted 40 mL Dulbecco’s phosphate-buffered saline. None associated adverse events as infusion reactions (allergic or otherwise) changes vital signs. Clinical lab serum neurofilament cytokine levels measured prior each varied somewhat without clear trend. A more sensitive in-house assay suggested possible inflammasome activation disease course. trend clinical stabilization was observed period. Our study provides novel approach address possibly patients vesicles. Initial findings suggest this safe.
Language: Английский
Citations
6
Journal of Controlled Release, Journal Year: 2024, Volume and Issue: 372, P. 209 - 220
Published: June 22, 2024
Retinal diseases are the leading cause of blindness, resulting in irreversible degeneration and death retinal neurons. One such cell type, ganglion (RGC), is responsible for connecting retina to rest brain through its axons that make up optic nerve primary lost glaucoma traumatic neuropathy. To date, different therapeutic strategies have been investigated protect RGCs from preserve vision, yet currently available restricted treating neuron loss by reducing intraocular pressure. A major barrier identified these studies drug delivery RGCs, which large part due stability, short duration time at target, low efficiency, undesired off-target effects. Therefore, a system deal with problems needed ensure maximum benefit candidate material. Extracellular vesicles (EV), nanocarriers released all cells, lipid membranes encapsulating RNAs, proteins, lipids. As they naturally shuttle encapsulated compounds between cells communicative purposes, may be exploitable offer opportunities overcome hurdles delivery, including molecular weight, barriers retina, adverse Here, we summarize potential an EV system, discussing their superiorities application target RGCs.
Language: Английский
Citations
4
Neurochemical Research, Journal Year: 2025, Volume and Issue: 50(2)
Published: March 18, 2025
Language: Английский
Citations
0
Biomolecules, Journal Year: 2025, Volume and Issue: 15(2), P. 219 - 219
Published: Feb. 3, 2025
This study evaluated the neuroprotective potential of peptain-1 conjugated to a cell-penetrating peptide (CPP-P1) in an ocular hypertension model glaucoma. Brown Norway (BN) rats were subjected intraocular pressure (IOP) elevation via intracameral injection silicone oil (SO), with concurrent intravitreal injections either CPP-P1 or vehicle. Retinal cross-sections analyzed for markers neuroprotection, including cAMP response element-binding protein (CREB), phosphorylated CREB (p-CREB), growth-associated protein-43 (GAP43), synapsin-1 (SYN1), and superoxide dismutase 2 (SOD2). Hematoxylin eosin staining was used assess retinal-layer thickness. SO-treated exhibited significant reductions thickness inner nuclear layer (INL, 41%, p = 0.016), plexiform (IPL, 52%, 0.0002), ganglion cell (GCL, 57%, 0.001). treatment mitigated these reductions, preserving INL by 32% (p 0.059), IPL 19% 0.119), GCL 31% 0.057). Increased levels 0.17) p-CREB 0.04) observed IOP-elevated, CPP-P1-treated retinas compared vehicle-treated retinas. Although overall GAP43 low, there modest increase expression within SO- 0.15 0.09, respectively) SO reduced SYN1 both 0.01), whereas significantly increased 0.03) 0.002). While SOD2 minimal across all groups, trend toward animals 0.16). The replicated removal after 7 days monitored 21 followed retinal flat-mount preparation (RGC) survival. A 42% loss RGCs 0.009) SO-injected eyes, which approximately 37% treatment. These findings suggest that is promising agent promotes survival preservation other neurons, potentially through enhanced signaling rat SO-induced hypertension.
Language: Английский
Citations
0
Advanced Healthcare Materials, Journal Year: 2025, Volume and Issue: unknown
Published: March 25, 2025
Retinal ganglion cell (RGC) degeneration leads to irreversible blindness. Müller glia (MG) play pivotal roles in retinal homeostasis and disease through paracrine signaling. Small extracellular vesicles (sEVs) are bioactive nanomaterials derived from all types of live cells recognized as a potential strategy for neuroprotective therapy. The aim this study is investigate the MG-derived sEVs (MG-sEVs) mouse model optic nerve injury (ONC). It found that MG-sEVs treatment effectively mitigates RGC suppresses microglial activation, thereby improves visual function ONC mice. transcriptomic analysis reveals strong correlation between C-x3-c motif chemokine ligand 1 (Cx3cl1)-mediated glial activation inflammation. Subsequently, it confirmed expression levels Cx3cl1 proinflammatory cytokines significantly decreased retinas treated with MG-sEVs. components cargo identifies miR-125b-5p miR-16-5p target gene regulate its expression. also observed colocalizes on microglia transgenic receptor (Cx3Cr1)-GFP In conclusion, mitigate by suppressing via Cx3cl1-Cx3cr1 This research provides additional opportunities degeneration.
Language: Английский
Citations
0
Investigative Ophthalmology & Visual Science, Journal Year: 2025, Volume and Issue: 66(4), P. 16 - 16
Published: April 7, 2025
Traumatic optic neuropathy (TON) leads to the loss of retinal ganglion cells (RGCs) and results in permanent visual impairment. Protecting regenerating RGCs is crucial for treatment TON. Studies have demonstrated that astrocyte-derived extracellular vesicles (ADEVs) exhibit neuroprotective effects models central nervous system (CNS) injury. This study aimed investigate whether ADEVs a similar effect on an nerve crush (ONC) rat model. were collected from primary astrocytes, ONC model was established evaluate structure function using optical coherence tomography (OCT), hematoxylin eosin (H&E) staining, flash evoked potential (f-VEP) analysis. Immunofluorescence used examine reactive gliotic changes. Additionally, miRNA sequencing mRNA performed identify mechanisms involved. protected progressive improved function. also significantly increased expression glial fibrillary acidic protein (GFAP) modulated microglial activation. The miRNAs associated with targeted by signals, such as MAPK, PI3K-AKT, TNF-α, through targeting network generated via sequencing, we found functional genes, THBS1, PAK3, Gstm1, likely participate microenvironmental regulation. We discovered play role Our findings provide new cell-free therapeutic strategy neuropathy.
Language: Английский
Citations
0
Journal of Controlled Release, Journal Year: 2025, Volume and Issue: unknown, P. 113778 - 113778
Published: April 1, 2025
Language: Английский
Citations
0
Asian Journal of Pharmaceutical Sciences, Journal Year: 2024, Volume and Issue: 19(4), P. 100942 - 100942
Published: July 9, 2024
Asthma is a widespread public health concern, with an increasing incidence. Despite the implementation of current treatment strategies, asthma control, particularly for severe cases, remains suboptimal. Recent research has revealed encouraging prospects extracellular vesicles (EVs) secreted by mesenchymal stem cells (MSCs) as viable therapeutic option alleviating symptoms. Therefore, present review aims to provide overview progress and mechanisms using MSC-derived EVs (MSC-EVs) treatment. Additionally, different administration approaches their impacts on biodistribution curative outcomes are summarized. Notably, potential benefits nebulized inhalation MSC-EVs addressed. Also, possibilities challenges in clinics highlighted. Overall, this intended give new insight into utilization biological drug
Language: Английский
Citations
2