Qualitative and quantitative detection of SARS-CoV-2 antibodies from dried blood spots

Clinical Biochemistry, Journal Year: 2022, Volume and Issue: 117, P. 16 - 22

Published: Jan. 3, 2022

Language: Английский

---

A bead-based multiplex assay covering all coronaviruses pathogenic for humans for sensitive and specific surveillance of SARS-CoV-2 humoral immunity

Scientific Reports, Journal Year: 2023, Volume and Issue: 13(1)

Published: Dec. 9, 2023

Serological assays measuring antibodies against SARS-CoV-2 are key to describe the epidemiology, pathobiology or induction of immunity after infection vaccination. Of those, multiplex targeting multiple antigens especially helpful as closely related coronaviruses other can be analysed simultaneously from small sample volumes, hereby shedding light on patterns in immune response that would otherwise remain undetected. We established a bead-based 17-plex assay detecting all pathogenic for humans: SARS-CoV-2, SARS-CoV, MERS-CoV, HCoV strains 229E, OC43, HKU1, and NL63. The was validated five commercial serological immunoassays, surrogate virus neutralisation test, assay, SARS-CoV-2. It found highly versatile shown by antibody detection both serum dried blot spots three case studies. First, we followed seroconversion four endemic an outbreak study day-care centres children. Second, were able link more severe clinical course stronger IgG with this 17-plex-assay, which IgG1 IgG3 dominated. Finally, our discriminate recent previous infections calculating IgG/IgM ratio N antigen antibodies. In conclusion, due comprehensive method comparison, thorough validation, proven versatility, is valuable tool studies coronavirus serology.

Language: Английский

---

Performance of Immunoglobulin G Serology on Finger Prick Capillary Dried Blood Spot Samples to Detect a SARS-CoV-2 Antibody Response

Microbiology Spectrum, Journal Year: 2022, Volume and Issue: 10(2)

Published: March 10, 2022

We investigate the diagnostic accuracy and predictive value of finger prick capillary dried blood spot (DBS) samples tested by a quantitative multiplex anti-immunoglobulin G (IgG) assay to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antibodies after infection or vaccination. This cross-sectional study involved participants (n = 6,841) from several serological surveys conducted in nonhospitalized children adults throughout 2020 2021 British Columbia (BC), Canada. Analysis used paired DBS serum subset 642) prior vaccination establish signal thresholds calculate logistic regression. Discrimination regression model was assessed receiver operator curve (ROC) analysis an n 2,000 bootstrap sample 642). The cross-validated vaccinated persons 90). Unpaired 6,723) were evaluate anti-IgG distributions. In comparison serum, unvaccinated population possessed sensitivity 79% (95% confidence interval [95% CI]: 58 91%) specificity 97% CI: 95 98%). ROC found that accurately classify SARS-CoV-2 seroconversion at 88% percent rate (area under [AUC] 80 95%]). disease 2019 (COVID-19) vaccine dose one two recipients, testing increased 83 99%) 100% 88 100%). Modeling possesses high positive (98% 97 98%]) with 75% seroprevalence. demonstrate should be considered reliably seropositivity natural IMPORTANCE Dried have comparable collected venipuncture when electrochemiluminescent for following and/or

Language: Английский

---

Comparison of a Blood Self-Collection System with Routine Phlebotomy for SARS-CoV-2 Antibody Testing

Diagnostics, Journal Year: 2022, Volume and Issue: 12(8), P. 1857 - 1857

Published: July 31, 2022

The Coronavirus Disease 2019 (COVID-19) pandemic forced researchers to reconsider in-person assessments due transmission risk. We conducted a pilot study evaluate the feasibility of using Tasso-SST (Tasso, Inc, Seattle, Washington) device for blood self-collection use in SARS-CoV-2 antibody testing an ongoing COVID-19 prevalence and immunity research study. 100 participants were recruited between January March 2021 from previously identified sub-cohort Cabarrus County Prevalence Immunity (C3PI) Study who under-going bimonthly testing. Participants given kit asked self-collect during scheduled visit where trained laboratory personnel performed routine phlebotomy. All completed after-visit survey about their experience. Overall, 70.0% able collect adequate sample device. Among those with sample, there was high concordance results phlebotomy collection methods (Cohen’s kappa coefficient = 0.88, Interclass correlation 0.98 [0.97, 0.99], p < 0.0001). received high-level (90.0%) acceptance among all participants. could prove be valuable tool seroprevalence However, future studies larger, diverse populations over longer periods may provide better understanding usability older comorbidities various scenarios.

Language: Английский

---

Applications of SARS-CoV-2 serological testing: impact of test performance, sample matrices, and patient characteristics

Critical Reviews in Clinical Laboratory Sciences, Journal Year: 2023, Volume and Issue: 61(1), P. 70 - 88

Published: Oct. 6, 2023

AbstractLaboratory testing has been a key tool in managing the SARS-CoV-2 global pandemic. While rapid antigen and PCR proven useful for diagnosing acute infections, additional methods are required to understand long-term impact of infections on immune response. Serological testing, well-documented laboratory practice, measures presence antibodies sample uncover information about host immunity. Although proposed applications serological clinical use have previously limited, current research into shown growing utility these settings. To name few, used identify patients with past active disease monitor vaccine efficacy. Test result interpretation, however, often complicated by factors that include poor test sensitivity early infection, lack response some individuals, overlying infection vaccination responses, standardization antibody titers/levels between instruments, unknown titers confer protection, large between-individual biological variation following or vaccination. Thus, three major components this review will examine (1) affect utility: performance, matrices, seroprevalence concerns viral variants, (2) patient response: timing sampling, age, sex, body mass index, immunosuppression vaccination, (3) informative testing: identifying surveillance guide health practices, examination protective should be beneficial care if it is implemented appropriately. However, as other developed tests, serology modality warrants careful consideration limitations evaluation its utility.Keywords: SARS-CoV-2COVID-19serologyimmunityantibody Disclosure statementJT receives in-kind support from Roche Diagnostics.Additional informationFundingThis open-access article supported funding Canadian Institutes Health Research (Funding Reference Number VR4-172753, VS1-175526, VS2-175572.

Language: Английский

---