Photoresponsive ‘Chemo-Free’ Phytotherapy: Formulation Development For the Treatment of Triple-Negative Breast Cancer DOI
Neha Maheshwari, Mukesh C. Sharma

Nanomedicine, Journal Year: 2024, Volume and Issue: 19(1), P. 5 - 24

Published: Jan. 1, 2024

Aim: The present investigation aimed to develop a chemo-free, nanophytosomal system treat triple-negative breast cancer (TNBC) via phyto–photo dual treatment strategy. Method: Size, shape, surface analysis, photoprovoked release profile, photothermal stability, (3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide assay, apoptotic DNA fragmentation, in vitro cellular uptake evaluation, mitochondrial membrane potential and caspase-3 photodynamic evaluation. Results: Biological experiments using MDA-MB-231 cells displayed dose-dependent synergistic anti-TNBC activity of PhytoS/Houttuynia cordata extract (HCE)/IR780 as compared with Phyto/HCE, PhytoS/IR780 even more promising under laser treatment. Apoptotic assay fragmentation analysis also showed enhanced effects. Investigation found that HCE acts suppression inducing cells. Conclusion: Our findings suggested photo-empowered phytotherapy can be employed effectively safely against TNBC.

Language: Английский

Exploring Elastic Lipid Nanovesicles for Enhanced Skin Permeation of Whole Plant Extract: A Comprehensive Investigation DOI Open Access
Rahul Maheshwari, Mayank Sharma, Sankha Bhattacharya

et al.

Journal of Biomedical Materials Research Part B Applied Biomaterials, Journal Year: 2025, Volume and Issue: 113(1)

Published: Jan. 1, 2025

A new method is developed using elastic lipid nanovesicles (ELNs) loaded with ethanolic extract of Lantana camara (LC) to enhance skin permeation plant actives. The ELNs contained cholesterol, 1, 2-distearoyl-sn-glycero-3-phosphocholine, span 80, and tween 80. Firstly, 15 formulations were produced examine critical factors likely affecting formulation characteristics. In addition, surface characteristics, vesicle size, polydispersity index, zeta potential, degree deformability, % entrapment efficiency (% EE) ELN examined. As a significant parameter, was measured (using Start-M; it highly resembles human skin). influence hydrophilic-lipophilic balance (HLB), ratio vital through Start-M. particular, the LC decreased at more extensive size ranges, 400, 350-450 nm. contrast, increased significantly smaller such as 200 100 More than 75% permeated within 8 h when surfactant (span 80:tween 80; 25%:75%). Permeation studies conducted based on HLB values revealed that 78% in 12.2, an increase HLB. Cell viability assay SK-MEL-37 cells (skin cancer) reduced by ~80% 24 h, further validating formulation. Future research could investigate long-term safety therapeutic potential these clinical settings their effectiveness delivering other plant-based extracts for transdermal applications via ELNs.

Language: Английский

Citations

1

Anticancer Properties of Some Selected Plant Phenolic Compounds: Future Leads for Therapeutic Development DOI
Neha Maheshwari, Mukesh C. Sharma

Journal of Herbal Medicine, Journal Year: 2023, Volume and Issue: 42, P. 100801 - 100801

Published: Oct. 17, 2023

Language: Английский

Citations

16

New insights in aptamer-targeted nanoliposomes for the treatment of breast cancer DOI
Nidhi Jain Singhai, Rahul Maheshwari,

Kapil Khatri

et al.

Journal of Drug Delivery Science and Technology, Journal Year: 2023, Volume and Issue: 87, P. 104880 - 104880

Published: Aug. 26, 2023

Language: Английский

Citations

13

Lab on chip based self-adjustable liposomes for rapid wound healing: An in depth in vitro, in vivo and higher dose toxicity investigation DOI
Rahul Maheshwari,

Piyush Ghode,

Mayank Sharma

et al.

Biomaterials Advances, Journal Year: 2024, Volume and Issue: 158, P. 213777 - 213777

Published: Jan. 19, 2024

Language: Английский

Citations

4

Photoresponsive ‘Chemo-Free’ Phytotherapy: Formulation Development For the Treatment of Triple-Negative Breast Cancer DOI
Neha Maheshwari, Mukesh C. Sharma

Nanomedicine, Journal Year: 2024, Volume and Issue: 19(1), P. 5 - 24

Published: Jan. 1, 2024

Aim: The present investigation aimed to develop a chemo-free, nanophytosomal system treat triple-negative breast cancer (TNBC) via phyto–photo dual treatment strategy. Method: Size, shape, surface analysis, photoprovoked release profile, photothermal stability, (3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide assay, apoptotic DNA fragmentation, in vitro cellular uptake evaluation, mitochondrial membrane potential and caspase-3 photodynamic evaluation. Results: Biological experiments using MDA-MB-231 cells displayed dose-dependent synergistic anti-TNBC activity of PhytoS/Houttuynia cordata extract (HCE)/IR780 as compared with Phyto/HCE, PhytoS/IR780 even more promising under laser treatment. Apoptotic assay fragmentation analysis also showed enhanced effects. Investigation found that HCE acts suppression inducing cells. Conclusion: Our findings suggested photo-empowered phytotherapy can be employed effectively safely against TNBC.

Language: Английский

Citations

1