Carbohydrate Polymer Technologies and Applications,
Journal Year:
2023,
Volume and Issue:
7, P. 100406 - 100406
Published: Dec. 10, 2023
The
extract
of
Citrus
aurantifolia
peel
has
been
encapsulated
into
alginate-gelatin-based
microbeads
using
an
external
ionotropic
gelation
method
to
apply
antibacterial
wound
dressings.
Such
variables,
like
the
alginate-gelatin
ratio,
quantity,
and
CaCl2
cross-linkers,
were
optimized
produce
a
good
yield,
well-distributed
size,
encapsulation
efficiency.
Antibacterial
efficacy
was
tested
against
both
S.
aureus
E.
coli.
Optimal
microbead
characteristics
obtained
at
alginate:gelatin
concentration
ratio
1.75%:0.25%
(w/v),
with
15
mg
2%
cross-linker.
These
conditions
resulted
in
82.94
±
1.056%
particle
sizes
between
1.080-1.244
µm,
efficiency
79.127
0.186%.
exhibited
almost
spherical
morphology
slightly
rough
surface.
release
mechanism
out
governed
by
diffusion
erosion
shells
following
Korsmeyer-Peppas
kinetic
model
(k=1.014
0.007/min).
Upon
immobilizing
on
gauze,
add-on
percentage
73.98%
observed.
Moreover,
gauze-immobilized
inhibited
coli
growth
inhibition
zones
11±1.48
12.56±4.89
mm,
respectively.
In
conclusion,
have
demonstrated
optimal
effective
coli,
showcasing
their
potential
as
Carbohydrate Polymer Technologies and Applications,
Journal Year:
2024,
Volume and Issue:
7, P. 100499 - 100499
Published: April 10, 2024
Aerogels
are
renowned
for
their
exceptional
characteristics
such
as
high
porosity
and
ultra-low
density.
Among
these,
pectin
aerogels,
offering
biodegradability,
low
toxicity,
versatility,
prominent
candidates
innovative
biomedical
materials.
This
study
focused
on
synthesizing
pectin-based
aerogels
carriers
ibuprofen
diclofenac
sodium.
Hydrogels
were
formed
by
combining
a
methoxy
with
calcium
chloride
solution,
followed
dehydration
drying
using
either
supercritical
carbon
dioxide
(scCO2)
or
freeze-drying.
Comparative
analysis
showed
scCO2-dried
exhibited
slightly
less
shrinkage
(0.27%)
than
freeze-dried
counterparts.
Both
types
mesoporous
characteristics.
However,
demonstrated
higher
specific
surface
area,
pore
volume,
smaller
diameter.
FTIR
spectra
indicated
no
interaction
between
the
drugs
aerogels.
The
loading
of
in
scCO2-dried,
at
90,
95,
100
bar
60.0%,
59.9%,
52.1%,
respectively,
solution
sodium
was
38.37%.
At
bar,
ibuprofen-loaded
released
90.0%,
84.0%,
75.5%,
respectively.
release
sodium-loaded,
88.4%.
Korsmeyer-Peppas
model
fitted
both
sodium,
indicating
is
mainly
driven
diffusion.
Scientific Reports,
Journal Year:
2024,
Volume and Issue:
14(1)
Published: June 18, 2024
Abstract
Hybrid
structures
made
of
natural-synthetic
polymers
have
been
interested
due
to
high
biological
features
combining
promising
physical–mechanical
properties.
In
this
research,
a
hybrid
dressing
consisting
silk
fibroin
(SF)/polyvinyl
alcohol
(PVA)
nanofibers
and
sodium
alginate
(SA)/gum
tragacanth
(GT)
hydrogel
incorporating
cardamom
extract
as
an
antibacterial
agent
was
prepared.
Accordingly,
SF
extracted
from
cocoons
followed
by
electrospinning
in
blend
form
with
PVA
(SF/PVA
ratio:
1:1)
under
the
voltage
18
kV
distances
15
cm.
The
SEM
images
confirmed
formation
uniform,
bead
free
fibers
average
diameter
199
±
28
nm.
FTIR
XRD
results
revealed
successful
extraction
preparation
mixed
fibrous
mats.
Next,
oil
extract-loaded
SA/GT
prepared
nanofibrous
structure
placed
on
surface
hydrogel.
analysis
depicted
uniform
morphology
desirable
matching
between
two
layers.
TGA
showed
desired
thermal
stability.
swelling
ratio
found
be
1251%
after
24
h
for
drug
released
without
any
initial
burst.
MTT
assay
cell
attachment
favorable
biocompatibility
proliferation
samples
containing
extract,
activity
values
85.35%
against
S.
aureus
75%
E.
coli
were
obtained
well.
that
engineered
nanofibrous-hydrogel
film
could
candidate
wound
healing
applications
skin
tissue
engineering.
International Journal of Nanomedicine,
Journal Year:
2023,
Volume and Issue:
Volume 18, P. 6275 - 6292
Published: Nov. 1, 2023
Background:
Skin
wound
is
a
widespread
health
problem
and
brings
extraordinary
burdens
to
patients.
Exosomes
derived
from
adipose-derived
stem
cells
(ADSC-Exos)
are
considered
promising
strategies
for
repairing
skin
wounds.
E2F1
member
of
the
E2F
family
transcription
factors
involved
in
cell
growth
apoptosis.
deficiency
mice
enhances
healing
by
improving
collagen
deposition
angiogenesis.
Additionally,
can
regulate
paracrine
activity
multiple
miRNAs,
which
will
inevitably
reshape
expression
profile
ADSC-Exos.
This
study
aimed
investigate
impact
factor
on
functions
ADSC-Exos
promoting
healing.
Methods:
First,
we
obtained
ADSCs
subcutaneous
adipose
tissues
WT
–/–
C57BL/6
separated
their
exosomes,
denoted
as
ADSC
-Exos
E2F1-/-
-Exos.
The
effects
full-thickness
models
were
investigated
images,
H&E
staining,
immunohistochemical
staining.
For
vitro
study,
abilities
promote
activities,
formation,
angiogenesis
evaluated.
potential
mechanism
was
determined
miRNA
sequencing,
ChIP‒qPCR,
dual-luciferase
assays.
Results:
accelerated
formation
As
result,
compared
with
lower
rate
30.5%
within
7
days
control
group
42.3%
-Exo
group,
significantly
increased
72.5%.
In
vitro,
activated
function
fibroblasts
vascular
endothelial
cells.
loss
promoted
miR-130b-5p
through
transcriptional
regulation.
MiRNA
high-throughput
sequencing
identified
12
differently
expressed
miRNAs
between
.
enhanced
fibroblast
activities
via
miR-130b-5p/TGFBR3
axis
TGF-β
activation.
Conclusion:
Our
results
indicated
that
effectively
regulating
axis,
thus
providing
novel
strategy
gene-engineered
exosomes
accelerating
Keywords:
healing,
cells,
exosome,
E2F1,
Carbohydrate Polymer Technologies and Applications,
Journal Year:
2023,
Volume and Issue:
7, P. 100406 - 100406
Published: Dec. 10, 2023
The
extract
of
Citrus
aurantifolia
peel
has
been
encapsulated
into
alginate-gelatin-based
microbeads
using
an
external
ionotropic
gelation
method
to
apply
antibacterial
wound
dressings.
Such
variables,
like
the
alginate-gelatin
ratio,
quantity,
and
CaCl2
cross-linkers,
were
optimized
produce
a
good
yield,
well-distributed
size,
encapsulation
efficiency.
Antibacterial
efficacy
was
tested
against
both
S.
aureus
E.
coli.
Optimal
microbead
characteristics
obtained
at
alginate:gelatin
concentration
ratio
1.75%:0.25%
(w/v),
with
15
mg
2%
cross-linker.
These
conditions
resulted
in
82.94
±
1.056%
particle
sizes
between
1.080-1.244
µm,
efficiency
79.127
0.186%.
exhibited
almost
spherical
morphology
slightly
rough
surface.
release
mechanism
out
governed
by
diffusion
erosion
shells
following
Korsmeyer-Peppas
kinetic
model
(k=1.014
0.007/min).
Upon
immobilizing
on
gauze,
add-on
percentage
73.98%
observed.
Moreover,
gauze-immobilized
inhibited
coli
growth
inhibition
zones
11±1.48
12.56±4.89
mm,
respectively.
In
conclusion,
have
demonstrated
optimal
effective
coli,
showcasing
their
potential
as
