Ligand-based Exosome Affinity Purification (LEAP) Column Chromatography: A Tool for Clinical Applications DOI Creative Commons
Nadiar M. Mussin, Akmaral Baspakova, Kulyash R. Zhilisbayeva

et al.

West Kazakhstan Medical Journal, Journal Year: 2024, Volume and Issue: 66(4), P. 365 - 372

Published: Dec. 20, 2024

Exosomes are nano-sized extracellular vesicles that play essential roles in intercellular communication, carrying biomolecules such as proteins, lipids, and RNAs can influence physiological pathological processes. The isolation of pure exosomes is critical for both basic research clinical applications, including diagnostics therapeutics. Traditional exosome techniques, ultracentrifugation, lack specificity may yield impure samples, making the need advanced techniques evident. Ligand-based affinity purification (LEAP) column chromatography has emerged a novel method utilizes specific ligands targeting surface markers, providing highly specific, gentle, scalable approach to isolation. This mini review explores LEAP chromatography’s mechanism, benefits, potential emphasizing its g.rowing importance exosome-based therapies.

Language: Английский

Therapeutic Potential of Extracellular Vesicles (Exosomes) Derived From Platelet‐Rich Plasma: A Literature Review DOI Creative Commons
Aditya K. Gupta, Tong Wang, Jeffrey A. Rapaport

et al.

Journal of Cosmetic Dermatology, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 1, 2024

ABSTRACT Background There are exciting advances in the field of exosomes research as a potential regenerative therapy. Platelet‐rich plasma represents an abundant source exosomes. Aim To present evidence on use platelet‐rich plasma‐derived dermatology and discuss current technical limitations. Methods A literature search was conducted using Embase (Ovid), Web Science, ClinicalTrials.gov June 2024. Results contain myriad growth factors, genetic materials, lipids that mediate paracrine signaling. Several preclinical studies have demonstrated their wound healing. This includes faster contraction diabetic animal models, well enhanced angiogenesis, increased cell proliferation migration, protection against hyperglycemic conditions vitro. case study reported topical platelet‐derived exosome product tried successfully patient with persistent scalp wounds. In contrast, there is scarce information hair skin rejuvenation. Isolation techniques, activation methods, methods delivery not been optimized, which warrants further research. Novel such hydrogel‐based preparations, may enable application Conclusion Exosomes derived from therapeutic potential. More needed to standardize its use.

Language: Английский

Citations

1
Ligand-based Exosome Affinity Purification (LEAP) Column Chromatography: A Tool for Clinical Applications DOI Creative Commons
Nadiar M. Mussin, Akmaral Baspakova, Kulyash R. Zhilisbayeva

et al.

West Kazakhstan Medical Journal, Journal Year: 2024, Volume and Issue: 66(4), P. 365 - 372

Published: Dec. 20, 2024

Exosomes are nano-sized extracellular vesicles that play essential roles in intercellular communication, carrying biomolecules such as proteins, lipids, and RNAs can influence physiological pathological processes. The isolation of pure exosomes is critical for both basic research clinical applications, including diagnostics therapeutics. Traditional exosome techniques, ultracentrifugation, lack specificity may yield impure samples, making the need advanced techniques evident. Ligand-based affinity purification (LEAP) column chromatography has emerged a novel method utilizes specific ligands targeting surface markers, providing highly specific, gentle, scalable approach to isolation. This mini review explores LEAP chromatography’s mechanism, benefits, potential emphasizing its g.rowing importance exosome-based therapies.

Language: Английский

Citations

0