Efficient Production of the Colorless Carotenoid Phytoene in Yarrowia lipolytica through Metabolic Engineering DOI

Kwanghyun Park,

Gyeongmin Kim,

Sungman Cha

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 19, 2024

Phytoene, a colorless carotenoid with unique ultraviolet (UV)–B absorption properties, offers potential for applications in functional food, cosmetics, and therapeutics. However, their low natural yield poses challenge large-scale production. This study aims to enhance phytoene production the oleaginous yeast Yarrowia lipolytica by introducing heterologous synthase gene combined metabolic engineering approaches. We enhanced synthesis overexpressing key genes mevalonate pathway compartmentalizing biosynthetic within peroxisomes. Moreover, we inhibited glyoxylate cycle increase accumulation of peroxisomal acetyl-CoA available Our engineered strains demonstrated significant production, reaching up 1.34 g/L titer 58.74 mg/gDCW flask-scale fed-batch culture, which are highest levels reported date. These results underscore Y. as robust platform producing phytoenes other terpenoids on an industrial scale, offering valuable insights future efforts engineering.

Language: Английский

The state of the art in anti-aging: plant-based phytochemicals for skin care DOI Creative Commons
Merve Tomaş, Deniz Günal‐Köroğlu, Senem Kamiloğlu

et al.

Immunity & Ageing, Journal Year: 2025, Volume and Issue: 22(1)

Published: Jan. 31, 2025

Phytochemicals help mitigate skin aging by scavenging free radicals, modulating key enzymatic pathways, and promoting the skin's structural integrity. Carotenoids, vitamins, essential fatty acids, phenolic compounds work acting as antioxidants, inhibiting enzymes like hyaluronidase, collagenase, elastase, which degrade structure, reducing levels of inflammatory markers (IL-6, IL-8, etc.) matrix metalloproteinases (MMP-1, MMP-2) linked to aging. Recent research highlights that plant-based phytochemicals can improve elasticity, reduce hyperpigmentation, prevent breakdown important proteins, support wound healing, making them valuable components for care treatments. This review explores multifaceted roles in maintaining improving health, highlighting their mechanisms action potential anti-aging innovations.

Language: Английский

Citations

1
Critical Review on Orally Administered Nutricosmetics: Food-Based Solutions Conferring Skin Health from the inside out DOI
Yongxin Ren, Dongcheng Liu, Baojun Xu

et al.

Trends in Food Science & Technology, Journal Year: 2025, Volume and Issue: unknown, P. 104946 - 104946

Published: Feb. 1, 2025

Language: Английский

Citations

0
Instant and Multifunctional Nanofibers Loaded with Proanthocyanidins and Hyaluronic Acid for Skincare Applications DOI Creative Commons
Xuan Yang,

Pengcheng Gu,

Qiang Jiang

et al.

Biomedicines, Journal Year: 2024, Volume and Issue: 12(7), P. 1584 - 1584

Published: July 17, 2024

Hyaluronic-acid- and silk-fibroin-based nanofibrous mats loaded with proanthocyanidins collagen peptides were fabricated as multifunctional facial masks using electrospinning. Their morphology, hygroscopicity moisture retention, DPPH, ABTS free radical scavenging abilities, cytocompatibility investigated. The results showed that the dense uniform, an average diameter ranging from 300 to 370 nm. exhibited satisfactory oxidation resistance, biocompatibility, especially excellent capacities. DPPH activity was 90% 15 mg/L nanofibers, 0.005 nanofibers. protected fibroblasts oxidative stress damage induced by tert-butyl hydroperoxide (t-BHP) significantly promoted their proliferation. Compared traditional liquid semi-solid masks, prepared in this study contained fewer additives, which has significant advantages terms of safety. rapidly dissolved within 5 s after being sprayed water, facilitated release penetration active ingredients for skincare. Therefore, displayed indicating promising translational potential providing a valuable reference

Language: Английский

Citations

3
Electronic nose investigation and antioxidant assessment of CHARMS™ skincare cosmetics toward skin tone improvement DOI Creative Commons
Norhayati Muhammad,

Muhammad Suhaimi Sulong,

Mohd Fadzelly Abu Bakar

et al.

Deleted Journal, Journal Year: 2024, Volume and Issue: unknown, P. 100060 - 100060

Published: Dec. 1, 2024

Language: Английский

Citations

1
Efficient Production of the Colorless Carotenoid Phytoene in Yarrowia lipolytica through Metabolic Engineering DOI

Kwanghyun Park,

Gyeongmin Kim,

Sungman Cha

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 19, 2024

Phytoene, a colorless carotenoid with unique ultraviolet (UV)–B absorption properties, offers potential for applications in functional food, cosmetics, and therapeutics. However, their low natural yield poses challenge large-scale production. This study aims to enhance phytoene production the oleaginous yeast Yarrowia lipolytica by introducing heterologous synthase gene combined metabolic engineering approaches. We enhanced synthesis overexpressing key genes mevalonate pathway compartmentalizing biosynthetic within peroxisomes. Moreover, we inhibited glyoxylate cycle increase accumulation of peroxisomal acetyl-CoA available Our engineered strains demonstrated significant production, reaching up 1.34 g/L titer 58.74 mg/gDCW flask-scale fed-batch culture, which are highest levels reported date. These results underscore Y. as robust platform producing phytoenes other terpenoids on an industrial scale, offering valuable insights future efforts engineering.

Language: Английский

Citations

0