Microbial community structural and functional differentiation in capped thickened oil sands tailings planted with native boreal species DOI Creative Commons
Abdul Samad, Dani Degenhardt, Armand Séguin

et al.

Frontiers in Microbiology, Journal Year: 2023, Volume and Issue: 14

Published: July 3, 2023

The oil sands mining operations in Alberta have produced billions of m3 tailings which must be reclaimed and integrated into various mine closure landforms, including terrestrial landforms. Microorganisms play a central role nutrient cycling during the reclamation disturbed landscapes, contributing to successful vegetation restoration long-term sustainability. However, microbial community succession response reconstructed revegetated remain largely unexplored. This study aimed monitor structural functional responses communities subjected different capping strategies over two growing seasons (GS). To achieve this, column-based greenhouse experiment was conducted investigate that were capped with layer (10 or 30 cm) peat-mineral mix (PMM) planted either upland wetland communities. DNA metabarcoding analysis bacterial 16S rRNA gene fungal ITS2 region as well shotgun metagenomics used asses impact treatments on taxonomy functions, respectively. Results showed diversity composition changed considerably after GS compared baseline samples, while PMM much more stable. Likewise, several functions significantly enriched GS. Interestingly, varied depending plant community, leading higher number differentially abundant taxa decrease Shannon evenness treatment but not treatment. Moreover, presence increased energy-related metabolic (carbon, nitrogen, sulfur), these depleted by Fungi represented small proportion tailings, relative abundance time, favored growth some beneficial taxa, notably root endophyte Serendipita, both columns. results suggest selecting right combination material type may contribute improve below-ground processes sustain harsh environments such tailings.

Language: Английский

Patterns of bacterial communities in the rhizosphere and rhizoplane of alpine wet meadows DOI
Awais Iqbal, Muhammad Maqsood Ur Rehman, Wasim Sajjad

et al.

Environmental Research, Journal Year: 2023, Volume and Issue: 241, P. 117672 - 117672

Published: Nov. 18, 2023

Language: Английский

Citations

6
Structural diversity of bacterial communities in two divergent sunflower rhizosphere soils DOI Creative Commons
Blessing Chidinma Nwachukwu, Ayansina Segun Ayangbenro, Olubukola Oluranti Babalola

et al.

Annals of Microbiology, Journal Year: 2023, Volume and Issue: 73(1)

Published: Feb. 17, 2023

Abstract Purpose Farming practices on farmlands aim to improve nutrients in the fields or crops, soil quality and functions, as well boost sustain crop yield; however, effect of loss ecological diversity degradation have impacted ecosystem functions. The beneficial rhizosphere-microorganism network rotation may enhance a stable ecosystem. use next-generation sequencing technique will help characterize entire bacterial species sunflower rhizosphere compared with nearby bulk soils. We investigated potential community structure soils cultivated under different agricultural at two geographical locations North West Province South Africa. Methods DNA was extracted from associated plants (rhizosphere Lichtenburg (LTR) (LTB) mono-cropping Krayburg (KRPR) (KRPB) sites, sequenced employing 16S amplicon sequencing. Bioinformatics tools were used analyse dataset. Results Proteobacteria Planctomycetes dominated rhizosphere, while Firmicutes Actinobacteria predominant Significant differences phyla family levels predicted functional categories between ( P < 0.05) across sites revealed. physicochemical parameters observed influence dispersal sites. Conclusion This study provides information their predictive attributes growing stage, which suggests future for imminent production management enhanced yields.

Language: Английский

Citations

5
Importance of carbon and nitrogen availability to microbial necromass carbon accumulation in the drawdown area DOI

Shanshan Liao,

Xiaodong Nie,

Aoqi Zeng

et al.

CATENA, Journal Year: 2023, Volume and Issue: 235, P. 107688 - 107688

Published: Nov. 18, 2023

Language: Английский

Citations

5
Two Novel Genera, Neostemphylium and Scleromyces (Pleosporaceae) from Freshwater Sediments and Their Global Biogeography DOI Creative Commons
Daniel Torres-Garcia, Dania García, J. Cano

et al.

Journal of Fungi, Journal Year: 2022, Volume and Issue: 8(8), P. 868 - 868

Published: Aug. 17, 2022

Although the Pleosporaceae is one of species-richest families in Pleosporales, research into less-explored substrates can contribute to widening knowledge its diversity. In our ongoing survey on culturable Ascomycota from freshwater sediments Spain, several pleosporacean specimens taxonomic interest were isolated. Phylogenetic analyses based five gene markers (ITS, LSU, gapdh, rbp2, and tef1) revealed that these fungi represent so far undescribed lineages, which are proposed as two novel genera family, i.e., Neostemphylium typified by polymorphum sp. nov., Scleromyces accommodate submersus nov. characterized production phaeodictyospores apically swollen darkened conidiogenous cells, presence a synanamorph consists cylindrical brown phragmoconidia growing terminally or laterally hyphae, ability produce secondary conidia microconidiation cycle. placed phylogenetically distant any family only produces sclerotium-like structures vitro. The geographic distribution ecology N. Sc. inferred metabarcoding data using GlobalFungi database. results suggest globally distributed fungus represented environmental sequences originating primarily soil samples collected Australia, Europe, USA, whereas less common species has been found associated with sequence an Australian sample. phylogenetic ITS1 ITS2 at least four dark taxa might be related Scleromyces. phylogeny presented here allows us resolve taxonomy genus Asteromyces member Pleosporaceae.

Language: Английский

Citations

8
Microbial community structural and functional differentiation in capped thickened oil sands tailings planted with native boreal species DOI Creative Commons
Abdul Samad, Dani Degenhardt, Armand Séguin

et al.

Frontiers in Microbiology, Journal Year: 2023, Volume and Issue: 14

Published: July 3, 2023

The oil sands mining operations in Alberta have produced billions of m3 tailings which must be reclaimed and integrated into various mine closure landforms, including terrestrial landforms. Microorganisms play a central role nutrient cycling during the reclamation disturbed landscapes, contributing to successful vegetation restoration long-term sustainability. However, microbial community succession response reconstructed revegetated remain largely unexplored. This study aimed monitor structural functional responses communities subjected different capping strategies over two growing seasons (GS). To achieve this, column-based greenhouse experiment was conducted investigate that were capped with layer (10 or 30 cm) peat-mineral mix (PMM) planted either upland wetland communities. DNA metabarcoding analysis bacterial 16S rRNA gene fungal ITS2 region as well shotgun metagenomics used asses impact treatments on taxonomy functions, respectively. Results showed diversity composition changed considerably after GS compared baseline samples, while PMM much more stable. Likewise, several functions significantly enriched GS. Interestingly, varied depending plant community, leading higher number differentially abundant taxa decrease Shannon evenness treatment but not treatment. Moreover, presence increased energy-related metabolic (carbon, nitrogen, sulfur), these depleted by Fungi represented small proportion tailings, relative abundance time, favored growth some beneficial taxa, notably root endophyte Serendipita, both columns. results suggest selecting right combination material type may contribute improve below-ground processes sustain harsh environments such tailings.

Language: Английский

Citations

4