Osteoking inhibits apoptosis of BMSCs in osteoporotic rats via PI3K/AKT signaling pathway DOI

Guijiang Huang,

Wenjie Yin, Xin Zhao

et al.

Journal of Ethnopharmacology, Journal Year: 2024, Volume and Issue: 340, P. 118961 - 118961

Published: Dec. 7, 2024

Language: Английский

Scutellarin ameliorates mitochondrial dysfunction and apoptosis in OGD/R-insulted HT22 cells through mitophagy induction DOI Open Access
Lu Yang, Xianfeng Liu,

Siyin Chen

et al.

Biomedicine & Pharmacotherapy, Journal Year: 2024, Volume and Issue: 179, P. 117340 - 117340

Published: Aug. 27, 2024

Scutellarin (Scu), a flavonoid from herbal Erigeron breviscapus (Vaniot) Hand-Mazz, exerts neuroprotective effects against cerebral ischemia. However, whether the of Scu are related to mitochondrial protection needs further investigation. In this study, we aimed clarify mechanisms HT22 cells injury caused by oxygen-glucose deprivation and reperfusion (OGD/R). Our results proved that significantly reduced overload intracellular reactive oxygen species (cellar ROS) mitochondria (mito-ROS), ameliorating oxidative stress damage. TUNEL positive rate, Caspase-3 activity, Cytochrome c (Cyto-c) expression remarkably decreased following treatment. Meanwhile, could maintain morphology reverse ultrastructure changes. And membrane potential (MMP), consumption rate (OCR), adenosine triphosphate (ATP) production Na

Language: Английский

Citations

11
Huayu Tongbi formula attenuates rheumatoid arthritis by inhibiting the HIF1A/VEGFA/ANGPT axis and suppressing angiogenesis DOI Creative Commons

Zifeng Chen,

Xiang Guo, Simin Wu

et al.

Phytomedicine, Journal Year: 2025, Volume and Issue: 139, P. 156479 - 156479

Published: Feb. 11, 2025

Angiogenesis, integral to the pathogenesis of rheumatoid arthritis (RA), contributes advancement synovial hyperplasia, pannus formation, and bone destruction. The precise effect Huayu Tongbi formula (HT), a proven traditional Chinese medicine for RA treatment, on angiogenesis remains uncertain. To investigate effects HT potential underlying molecular mechanisms. target spectrum pharmacological were screened using network pharmacology. In vitro, RA-FLS stimulated with CoCl2 simulate hypoxic conditions. Human umbilical vein endothelial cells (HUVECs) cultured conditioned medium (CM) from supplemented or HIF1A inhibitor LW-6 impact cell secretions angiogenesis, encompassing proliferation, migration, adhesion, lumen formation. Western blot was employed assess protein levels hypoxia-inducible factor 1 subunit alpha (HIF1A), vascular growth receptor 2 (VEGFR2), phosphorylated VEGFR2 (p-VEGFR2). Enzyme-linked immunosorbent assay (ELISA) utilized quantify VEGFA secretion, while quantitative real-time PCR (qRT‒PCR) measured mRNA expression VEGFA, Angiopoietin (ANGPT1), ANGPT2. vivo, collagen-induced (CIA) model established. Pathological changes vessel counts observed via hematoxylin-eosin (HE) staining, structure evaluated X-ray, mature immature vessels classified immunofluorescence, mechanism further verified immunohistochemistry ELISA. Liquid chromatography‒mass spectrometry (LC‒MS) identify active components drug-containing serum, metabolites identified by bioinformatics analysis. Network pharmacology analysis indicated targets in correlating VEGF pathways. reversed adhesion lines suppressed luminal formation HUVECs. (10 % 15 %) effectively reduced expression, attenuated its phosphorylation, downregulated ANGPT1, ANGPT2 levels. mitigated inflammation destruction, (CD31+/α-SMA-) (CD31+/α-SMA+) synovium, inhibited HIF1A-VEGFA-ANGPT axis. Finally, 19 serum successfully identified, we also unexpectedly discovered that might affect metabolic pathways such as proline tyrosine metabolism, which have been reported be involved angiogenesis. has promising alleviating at least part through axis inhibition, reduces cross-talk between Our results suggest is drug candidate treatment

Language: Английский

Citations

1
Mechanism of traditional Chinese medicine in elderly diabetes mellitus and a systematic review of its clinical application DOI Creative Commons
Qiqi Zhang,

Shiwan Hu,

Zishan Jin

et al.

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15

Published: March 6, 2024

Affected by aging, the elderly diabetes patients have many pathological characteristics different from young people, including more complications, vascular cognitive impairment, osteoporosis, and sarcopenia. This article will explore their pathogenesis mechanism of Traditional Chinese medicine (TCM) intervention, use method systematic review to evaluate clinical application TCM in diabetes.

Language: Английский

Citations

5
Formation of self-assembly aggregates in traditional Chinese medicine decoctions and their application in cancer treatments DOI Creative Commons
Chunqiu Fang, Yinghang Wang, Zhi Pan

et al.

RSC Advances, Journal Year: 2025, Volume and Issue: 15(7), P. 5476 - 5506

Published: Jan. 1, 2025

Traditional Chinese Medicine (TCM) formulas, based on the principles of medicine, have a long history and are widely applied in treatment diseases.

Language: Английский

Citations

0
Unraveling the potential of neuroinflammation and autophagy in schizophrenia DOI

Hongxia Tao,

Cheng‐Xiang Wang, Chuan Zou

et al.

European Journal of Pharmacology, Journal Year: 2025, Volume and Issue: 997, P. 177469 - 177469

Published: March 5, 2025

Language: Английский

Citations

0
Evaluation and the mechanism of ShengXian and JinShuiLiuJun decoction in the treatment of silicotic fibrosis: an integrated network pharmacology, Life Omics, and experimental validation study DOI

Yiwen Tang,

Bingbing Wu, Liyuan Zhao

et al.

Journal of Ethnopharmacology, Journal Year: 2024, Volume and Issue: unknown, P. 118909 - 118909

Published: Oct. 1, 2024

Language: Английский

Citations

1
Osteoking inhibits apoptosis of BMSCs in osteoporotic rats via PI3K/AKT signaling pathway DOI

Guijiang Huang,

Wenjie Yin, Xin Zhao

et al.

Journal of Ethnopharmacology, Journal Year: 2024, Volume and Issue: 340, P. 118961 - 118961

Published: Dec. 7, 2024

Language: Английский

Citations

1