Molecular docking‐guided in‐depth investigation of the biological activities and phytochemical and mineral profiles of endemic Phlomis capitata

Journal of the Science of Food and Agriculture, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 29, 2025

Abstract BACKGROUND Phlomis capitata is an endemic species of flowering aromatic and medicinal plant in the family Lamiaceae, native to regions Mediterranean nearby areas. Understanding chemical compounds present P. can reveal potential properties. The study examines quantification bioactive phytochemicals, antioxidant capacity enzyme inhibitory evaluation extract against key enzymes involved pathogenesis Alzheimer's, diabetes mellitus, epilepsy glaucoma for first time. mechanisms inhibition activity predominant were also interpreted by molecular docking studies. Chemical characterization was performed liquid chromatography‐tandem mass spectrometry (LC‐MS/MS) (phytochemical profile) inductively coupled plasma‐mass (mineral composition) analysis. Furthermore, binding interactions major phytochemicals with all investigated RESULTS LC‐MS/MS analysis revealed identification 19 predominated quinic acid (4.883 mg g −1 ), followed chlorogenic (4.36 vanilic (3.405 naringenin (2.571 ) cyranoside (1.101 ). It determined that mineral element rich (K, Ca, Al Mg) did not exceed toxicity limits. demonstrated remarkable activities order: 2,2′‐azino‐ bis (3‐ethylbenzothiazoline‐6‐sulfonic (IC 50 : 20.533 μg mL < 2,2‐diphenyl‐1‐picrylhydrazyl 23.151 N , ‐dimethyl‐ p ‐phenylenediamine 45.221 cupric reducing (0.889 Fe 3+ (0.969 ferric potency (0.974 Moreover, assays (acetylcholinesterase, butyrylcholinesterase, α ‐amylase, ‐glucosidase, human carbonic anhydrases I II), showed outstanding values 3.26, 7.15, 6.15, 6.81, 15.21 11.93 respectively). CONCLUSION In summary, findings show a versatile raw material be used pharmaceutical, cosmetic food industries develop products promote health. © 2025 Author(s). Journal Science Food Agriculture published John Wiley & Sons Ltd on behalf Society Industry.

Language: Английский

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Development of Polymeric Nanoparticles Loaded with Phlomis crinita Extract: A Promising Approach for Enhanced Wound Healing

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(5), P. 2124 - 2124

Published: Feb. 27, 2025

The use of nanoparticles improves the stability, solubility, and skin permeability natural compounds in skincare products. Based on these advantages, this study aimed to incorporate Phlomis crinita extract into polymeric improve its topical delivery for wound healing purposes. involved preparation PLGA PLGA-PEG (PCE-PLGA-NPs PCE-PLGA-PEG-NPs) using solvent displacement method, physicochemical biopharmaceutical characterization, tolerance studies by HET-CAM assay evaluation integrity parameters, vitro efficacy via a scratch experiment. prepared were nanometer-sized with spherical form demonstrated an encapsulation efficiency greater than 90%. major component (luteolin) was released following kinetic model hyperbola PCE-PLGA-PEG-NPs one-phase exponential association PCE-PLGA-NPs. Moreover, important luteolin observed, especially PCE-PLGA-PEG-NPs. Both formulations exhibited no irritation damaging effects integrity, suggesting their safety. Finally, results experiment 3T3-L1 cells revealed significant cell migration proliferation, improved compared free extract, demonstrating potential formulation treatment healing.

Language: Английский

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Fractionation, HRLC-MS assisted phytochemical profiling, quantification, and evaluation of the chronic wound healing potential of ethanolic extract of Ardisia solanacea (Poir.) Roxb.

Pharmacological Research - Natural Products, Journal Year: 2025, Volume and Issue: unknown, P. 100182 - 100182

Published: Feb. 1, 2025

Language: Английский

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Exploring Phlomis crinita extracts: HPLC analysis, phenolic content, antioxidant and antimicrobial potentials

International Journal of Secondary Metabolite, Journal Year: 2025, Volume and Issue: 12(2), P. 381 - 396

Published: March 19, 2025

Phlomis crinita Cav. (P. crinita), known as " Khayat el-djerah in Algerian folk medicine, is used for wound healing and abdominal pain relief. This study assessed the phytochemical profile, phenolic content, antimicrobial activity against five Gram-negative three Gram-positive clinical bacterial strains, well vitro antioxidant of hydroethanolic extracts from leaves (HLE), flowers (HFE), rhizomes (HRE) P. crinita. Fifteen compounds such four flavonoids, trans-cinnamic acid, six cinnamic acid derivatives, benzoic derivatives were identified first time by HPLC-DAD, with quantitative differences among analyzed parts. HRE exhibited high levels total phenolics (262.97 ± 16.2 μg GAE/mg DW) flavonoids (71.87 3.25 QE/mg DW), while HLE had highest flavonols content (18.89 5.12 DW). All demonstrated strong properties. potency, IC50 values 15.46 0.45 µg/mL (DPPH) 11.71 0.50 (ABTS). good reducing power (FRAP A0.5 = 40.07 2.82 µg/mL), showed best (Phenanthroline 7.88 1.63 µg/mL). revealed broad-spectrum antibacterial effects, most potent Enterococcus faecalis, a minimum inhibitory concentration (MIC) value 1.25 mg/mL. These results that could be useful source bioactive pharmaceutical food industry.

Language: Английский

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Evaluation of Dermal Wound Healing Potential: Phytochemical Characterization, Anti‐inflammatory, Antioxidant, and Antimicrobial Activities of Euphorbia guyoniana Boiss. & Reut. Latex

Chemistry & Biodiversity, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 4, 2024

Abstract This study investigates the wound‐healing potential of Euphorbia guyoniana latex (EGL) in male Wistar rats, along with its biochemical composition and biological activities. Phytochemical analysis identified moderate levels phenolics, flavonoids, tannins, HPLC revealing five phenolic compounds. EGL demonstrated strong antioxidant activity DPPH assays, surpassing ascorbic acid protecting red blood cells. Its performance ß‐carotene‐linoleic assay was robust, though FRAP results were weaker. also exhibited significant anti‐inflammatory activity, comparable to Acetylsalicylic acid, showed antibacterial effects against Listeria innocua. In Vivo , EGL‐infused ointments accelerated wound healing, reducing epithelialization periods 12–16 days, a higher contraction rate compared controls. The concludes that EGL, rich bioactive compounds, holds as promising natural agent for owing potent antioxidant, anti‐inflammatory, properties.

Language: Английский

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Antibacterial effect and mechanisms of action of forsythoside B, alone and in combination with antibiotics, against Acinetobacter baumannii and Pseudomonas aeruginosa

Phytomedicine, Journal Year: 2024, Volume and Issue: 135, P. 156038 - 156038

Published: Sept. 13, 2024

Language: Английский

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