Phytomedicine, Journal Year: 2024, Volume and Issue: 137, P. 156356 - 156356
Published: Dec. 30, 2024
Language: Английский
Journal of Cellular and Molecular Medicine, Journal Year: 2025, Volume and Issue: 29(4)
Published: Feb. 1, 2025
ABSTRACT Chronic atrophic gastritis (CAG) is a precancerous lesion characterised by gastric mucosal atrophy and inflammation. Identifying key molecular mechanisms potential therapeutic targets essential to improve patient outcomes. Key modules differentially expressed genes (DEGs) were recognised in the GSE153224 dataset using weighted gene co‐expression network analysis (WGCNA) examination of differential expression. IGFBP7 was identified as hub protein–protein interaction (PPI) expression validation. CAG patients’ blood parameters health status evaluated before after treatment vitamin C (VC). In addition, we investigated effects VC N‐methyl‐N′‐nitro‐N‐nitrosoguanidine (MNNG) on GES‐1 cells, including cell viability, apoptosis inflammatory angiogenic markers. WGCNA that blue module significantly associated with correlation coefficient 0.924. Among 93 overlapping genes, notably underexpressed selected gene. ROC confirmed high diagnostic performance . patients treated showed significant improvement improved health. vitro, increased reduced cytotoxicity lowered COX‐2 apoptosis‐related protein MNNG‐treated cells. Knockdown further influenced these effects. MNNG upregulated HIF‐1α/VEGF signalling proteins, which attenuated. Combined knockdown protective This study highlights regulatory role demonstrates their for improving mitigating
Language: Английский
Citations
0
Pharmaceuticals, Journal Year: 2025, Volume and Issue: 18(4), P. 537 - 537
Published: April 7, 2025
Background/Objectives: Periostracum Cicadae (PC) is commonly used to treat chronic atrophic gastritis (CAG), but its underlying mechanisms are unclear. We investigated the therapeutic effects, active ingredients and molecular of PC on CAG. Methods: analyzed components in serum extract by UHPLC-Q-Orbitrap-MS/MS. Then, we rat cell models assess impact CAG employed network pharmacology bioinformatics predict key targets ingredients. Finally, confirmed hub through experiments docking. Results: A total 22 were identified extract-containing using UHPLC-Q-Orbitrap MS/MS. Network combined with docking revealed that protective effect was primarily mediated three compounds: (Z)-akuammidine, chicoric acid, columbianadin. And c-Fos/c-Jun signaling pathways crucial therapy. inhibited vitality, migration, invasion, multiplication MC cells (model for CAG), induced apoptosis, caused G0/G1 phase cycle arrest. The expression level tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), interleukin-1 beta (IL-1β) gastrin 17 (G17) rats increased, while pepsinogen I (PG I) II II) decreased. After 12 weeks administration, these conditions significantly improved. not only reduced levels antigen KI-67 (Ki67) protein p53 (P53) also enhanced SRY-box Transcription Factor (SOX2). Simultaneously, down-regulated N-cadherin Vimentin up-regulating E-cadherin. Conclusions: epithelial–mesenchymal transition (EMT) via pathway, thereby providing benefits Our study elucidates material basis treating CAG, experimental evidence support clinical application.
Language: Английский
Citations
0
Frontiers in Cell and Developmental Biology, Journal Year: 2024, Volume and Issue: 12
Published: Dec. 10, 2024
Chronic atrophic gastritis (CAG) is a prevalent digestive system disease characterized by atrophy of the gastric mucosa and disappearance inherent glands. According to theory Correa’s cascade, CAG an important pathological stage in transformation from normal condition carcinoma. In recent years, global incidence has been increasing due pathogenic factors, including Helicobacter pylori infection, bile reflux, consumption processed meats. this review, we comprehensively described etiology clinical diagnosis CAG. We focused on elucidating regulatory mechanisms promising therapeutic targets CAG, with expectation providing insights theoretical support for future research
Language: Английский
Citations
2
Journal of Ethnopharmacology, Journal Year: 2024, Volume and Issue: 339, P. 119136 - 119136
Published: Nov. 21, 2024
Language: Английский
Citations
1
Phytomedicine, Journal Year: 2024, Volume and Issue: 137, P. 156356 - 156356
Published: Dec. 30, 2024
Language: Английский
Citations
1