Ecotoxicology and Environmental Safety,
Journal Year:
2024,
Volume and Issue:
276, P. 116288 - 116288
Published: April 5, 2024
Cylindrospermopsin
(CYN),
a
cyanobacterial
toxin,
has
been
detected
in
the
global
water
environment.
However,
information
concerning
potential
environmental
risk
of
CYN
is
limited,
since
majority
previous
studies
have
mainly
focused
on
adverse
health
effects
through
contaminated
drinking
water.
The
present
study
reported
that
at
environmentally
relevant
levels
(0.1-100
μg/L)
can
significantly
enhance
conjugative
transfer
RP4
plasmid
Escherichia
coli
genera,
wherein
application
10
μg/L
led
to
maximum
fold
change
∼6.5-
16
h
exposure.
Meanwhile,
evaluation
underlying
mechanisms
revealed
concentration
exposure
could
increase
oxidative
stress
bacterial
cells,
resulting
ROS
overproduction.
In
turn,
this
an
upregulation
antioxidant
enzyme-related
genes
avoid
attack.
Further,
inhibition
synthesis
glutathione
(GSH)
was
also
detected,
which
rapid
depletion
GSH
cells
and
thus
triggered
SOS
response
promoted
process.
Increase
cell
membrane
permeability,
expression
related
pilus
generation,
ATP
synthesis,
gene
were
observed.
These
results
highlight
impact
spread
antimicrobial
resistance
environments.
Ecotoxicology and Environmental Safety,
Journal Year:
2023,
Volume and Issue:
262, P. 115124 - 115124
Published: June 15, 2023
The
emergence
and
rapid
spread
of
antibiotic
resistance
seriously
compromise
the
clinical
efficacy
current
therapies,
representing
a
serious
public
health
threat
worldwide.
Generally,
drug-susceptible
bacteria
can
acquire
through
genetic
mutation
or
gene
transfer,
among
which
horizontal
transfer
(HGT)
plays
dominant
role.
It
is
widely
acknowledged
that
sub-inhibitory
concentrations
antibiotics
are
key
drivers
in
promoting
transmission
resistance.
However,
accumulating
evidence
recent
years
has
shown
addition
to
antibiotics,
non-antibiotics
also
accelerate
genes
(ARGs).
Nevertheless,
roles
potential
mechanisms
non-antibiotic
factors
ARGs
remain
largely
underestimated.
In
this
review,
we
depict
four
pathways
HGT
their
differences,
including
conjugation,
transformation,
transduction
vesiduction.
We
summarize
accounting
for
enhanced
underlying
molecular
mechanisms.
Finally,
discuss
limitations
implications
studies.
ACS ES&T Water,
Journal Year:
2022,
Volume and Issue:
2(12), P. 2273 - 2288
Published: Nov. 4, 2022
Antibiotic
resistance
genes
(ARGs)
have
been
identified
as
emerging
contaminants
in
water
and
wastewater.
Concerns
raised
of
whether
disinfection
processes
can
effectively
eliminate
ARGs
thus
mitigate
their
spread.
Due
to
the
different
existing
forms
dissemination
pathways,
intracellular
(iARGs)
extracellular
(eARGs)
responses
processes.
This
review
compares
elimination
iARGs
eARGs
representative
applied
at
wastewater
treatment
plants,
including
by
chlorine,
chloramine,
chlorine
dioxide,
ozone,
ultraviolet
irradiation
(UV),
combination
UV
with
chemicals.
Meanwhile,
redistribution
upon
is
highlighted.
Elimination
efficiencies
generally
follow
order:
ozone
>
≫
dioxide
chloramine.
The
naked
could
be
up
4-log
higher
than
that
iARGs.
Notably,
these
disinfectants
resulting
byproducts
formed
process
select
antibiotic
resistant
bacteria
(ARB)
increase
relative
abundances
Under
practical
disinfectant
exposure,
elevated
may
observed.
Low
doses
chloramine
tend
enhance
transfer
from
forms.
To
selectively
control
associated
risks
meanwhile
minimize
unintended
consequences,
practice
warrant
optimization.
The Science of The Total Environment,
Journal Year:
2022,
Volume and Issue:
858, P. 159846 - 159846
Published: Oct. 31, 2022
Current
in
the
milliampere
range
can
be
used
for
electrochemical
inactivation
of
bacteria.
Yet,
bacteria-including
antibiotic
resistant
bacteria
(ARB)
may
subjected
to
sublethal
conditions
due
imperfect
mixing
or
energy
savings
measures
during
disinfection.
It
is
not
known
whether
such
current
intensities
have
potential
stimulate
plasmid
transfer
from
ARB.
In
this
study,
conjugal
pKJK5
was
investigated
between
Pseudomonas
putida
strains
under
reflecting
Although
abundance
culturable
and
membrane-intact
donor
recipient
cells
decreased
with
applied
(0-60
mA),
both
transconjugant
density
frequency
increased.
Both
active
chlorine
superoxide
radicals
were
generated
electrolytically,
ROS
generation
induced.
addition,
we
detected
significant
over
expression
a
core
oxidative
stress
defense
gene
(ahpCF)
current.
Expression
selected
conjugation
related
genes
(traE,
traI,
trbJ,
trbL)
also
significantly
correlated
intensity.
accumulation,
SOS
response
subsequent
derepression
are
therefore
plausible
consequence
exposure.
These
findings
suggest
that
enhance
transfer,
it
essential
disinfection
(applied
voltage,
density,
time
mixing)
carefully
controlled
avoid
ARG
transmission.
