Recent Advances in Developing Optical and Electrochemical Sensors for Monitoring Thiram and Future Perspectives

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: 72(42), P. 23024 - 23038

Published: Oct. 13, 2024

Thiram, as one widely used dithiocarbamate pesticide, has been considered seriously detrimental to food safety and human health because of poor efficiency, nonstandard/superfluous usage, lack a targeting effect. Developing high-performance sensors for thirams is strongly needed. With the rapid development chemistry, biology, materials science, many have constructed thiram with high sensitivity selectivity. Regarding energy form signal, recognition mode, detection principle, recent advances in design construction optical electrochemical are summarized this review, including colorimetric, luminescent, chemiluminescent, sensors. The advantages disadvantages sensitivity, ability avoid interference, mechanism, signal output practicability clarified detail. Furthermore, challenges faced, effective restrictions, next direction proposed achieving more sensitive selective analysis less interference. We desire that review will supply solid theoretical basis inspiration generate innovative thinking new progress on assays commercialization developed future.

Language: Английский

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Thiram exposure induces tibial dyschondroplasia in broilers via the regulation effect of circ_003084/miR-130c-5p/BMPR1A crosstalk on chondrocyte proliferation and differentiation

Journal of Hazardous Materials, Journal Year: 2023, Volume and Issue: 465, P. 133071 - 133071

Published: Nov. 24, 2023

Thiram, an agricultural insecticide, has been demonstrated to induce tibial dyschondroplasia (TD) in avian species. Circular RNA (circRNAs), a novel class of functional biological macromolecules characterized by their distinct circular structure, play crucial roles various processes and diseases. Nevertheless, the precise regulatory mechanism underlying non-coding involvement thiram-induced broiler chondrodysplasia remains elusive. In this study, we established model thiram exposure for 10 days assess TD obtain ceRNA network sequencing. By analyzing differentially expressed circRNAs network, id entify that circ_003084 was significantly upregulated cartilage. Elevated inhibited chondrocytes proliferation differentiation vitro but promote apoptosis. Mechanistically, competitively binds miR-130c-5p prevents decrease level BMPR1A, which upregulates expression apoptosis genes Caspase 3, 9, Bax Bcl

Language: Английский

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Impact of Light Wavelength on Growth and Welfare of Broiler Chickens – Overview and Future Perspective

Annals of Animal Science, Journal Year: 2023, Volume and Issue: 24(3), P. 731 - 748

Published: Oct. 5, 2023

Abstract Lighting in poultry production holds notable importance with the advancement and modernization of industry, as it plays a significant role physiology growth broiler chickens. Increasing attention has been paid to impacts lighting management on performance, immune status, welfare meat-type It is essential have an appropriate regimen that includes light source, intensity, duration, wavelength improve broilers’ behavior. By manipulating various physiological, immunological, behavioral activities, altering color acknowledged potent managerial strategy capable mitigating array stressors Assessing animal necessary for behavior product quality perspectives. Birds unique visual system, their primarily mediated by vision. Different monochromatic regimes can affect feed conversion ratio, modulating chickens’ systematic response aggressive With technology, new possibilities emerged enhance traditional programs houses. This review integrates recent findings use its impact welfare, growth, physiological response.

Language: Английский

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Impact of the arginine silicate inositol complex on bone metabolism in broiler chickens with tibial dyschondroplasia caused by manganese deficiency

British Poultry Science, Journal Year: 2024, Volume and Issue: 65(4), P. 455 - 464

Published: April 10, 2024

1. Tibial dyschondroplasia (TD) is a skeletal disorder in broilers that has financial implications, necessitating dietary modifications to reduce the prevalence of this disease. This study explored how arginine silicate inositol complex (ASI) supplementation affected tibial growth plate (TGP) and overall bone health with manganese (Mn) deficiency-induced TD.2. A total 240 broiler chicks were divided into four groups, each consisting 60 birds (15 replicates each) as follows: i) Control, mg Mn per kg diet; ii) ASI, 1 g ASI iii) TD, 22 diet, iv) TD+ASI, diet.3. It was found increased length Mn-deficient TD (

Language: Английский

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Effects of Hypericum attenuatum Choisy extract on the immunologic function and intestinal microflora of broilers under oxidative stress

Poultry Science, Journal Year: 2024, Volume and Issue: 103(11), P. 104189 - 104189

Published: Aug. 8, 2024

This study investigated the impact of Hypericum attenuatum Choisy extract (HYG) on immunological function and cecum microflora in broilers. A total 240 one-day-old AA broilers were randomly divided into 5 groups with 6 replicates 8 each: 1) CN group, which injected saline fed a basal diet; 2) PC lipolyaccharide (LPS) 3) HYG1 LPS 400 mg/kg HYG-supplemented 4) HYG2 800 5) HYG3 1,200 diet. Broilers 1 or same amount 12 hours before sampling d 21 42. The results revealed that dietary HYG supplementation alleviated spleen index thymus abnormalities, balanced disturbance serum immunoglobulin (Ig)M IgA levels, regulated cytokine balance serum, liver, jejunum tissues included induced by LPS. Dietary also downregulated relative expression inhibitor kappa B kinase alpha (IKKα) interleukin (IL)-6 mRNAs liver upregulated (IκBα) IL-10 spleen. improved cecal at 42 increasing abundance beneficial bacteria, such as Alistipes Phascolarctobacterium, while reducing harmful Helicobacter Colidextribacter. Spearman correlation analysis negative between activation NF-κB inhibitory pathway presence Erysipelatoclostridium, Subdoligranulum Parabacteroides. Conclusions: incorporation diet was optimal improving broiler function.

Language: Английский

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Recent advances in the use of surface-enhanced Raman spectroscopy for thiram detection in food products

Journal of Food Composition and Analysis, Journal Year: 2024, Volume and Issue: 136, P. 106855 - 106855

Published: Oct. 13, 2024

Language: Английский

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Homeostatic Regulation of Pro-Angiogenic and Anti-Angiogenic Proteins via Hedgehog, Notch Grid, and Ephrin Signaling in Tibial Dyschondroplasia

Animals, Journal Year: 2023, Volume and Issue: 13(24), P. 3750 - 3750

Published: Dec. 5, 2023

Precise coupling of two fundamental mechanisms, chondrogenesis and osteogenesis via angiogenesis, plays a crucial role during rapid proliferation growth plates, alteration in their balance might lead to pathogenic conditions. Tibial dyschondroplasia (TD) is characterized by an avascular, non-mineralized, jade-white "cartilaginous wedge" with impaired endochondral ossification chondrocyte at the proximal end tibial bone rapidly growing poultry birds. Developing vascular structures are dynamic cartilage regulated through homeostatic among pro anti-angiogenic proteins cytokines. Pro-angiogenic factors involves wide spectrum multifactorial mitogens, such as endothelial (VEGF), platelet-derived (PDGF), basic fibroblast factor (bFGF), placental factors, transforming factor-β (TGF-β), TNF-α. Considering regulatory sonic hedgehog, notch-gridlock, ephrin-B2/EphB4 pathways inhibition like angiostatin, endostatin, decoy receptors, vasoinhibin, thrombospondin, PEX, troponin, possible persisting inflammatory conditions TD was studied current literature review. Balanced apoptosis angiogenesis vital for physiological growth. Any imbalance apoptotic, angiogenetic, pro-angiogenic, or ultimately leads pathological osteoarthritis. The review substantiate solid grounds developing innovative therapeutics diseases governed disproportion anti-angiogenesis proteins.

Language: Английский

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Preparation of Cellulose-Based Flexible Sers and its Application for Rapid and Ultra-Sensitive Detection of Thiram on Fruits and Vegetables

Published: Jan. 1, 2023

As one of the most common pesticide residues on surface fruits and vegetables, thiram has a serious threat to human health. Our team used an acidic hydrated metal salt low co-fusion solvent dissolve cellulose lysis slurry then successfully prepared regenerated membrane (RCM) by sol-gel method. Ag NPs with uniform particle size were deposited RCM continuous ion layer adsorption reaction (SILAR), finally NPs/RCM flexible SERS substrates prepared. The minimum detection limit this substrate for R6G is 5×10-9 M, shows good uniformity (RSD=12.58%) reproducibility (RSD=4.23%), sensitivity standard solution 10-10 M. Because its characteristics, can be applied three-dimensional environments such as fruit vegetable surfaces, practicality been confirmed in residue apples, tomatoes, pears other vegetables.

Language: Английский

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Transcriptome analysis reveals the pathogenesis of spontaneous tibial dyschondroplasia in broilers

Frontiers in Genetics, Journal Year: 2024, Volume and Issue: 15

Published: July 29, 2024

Tibial dyschondroplasia (TD) is a severe bone disease that affects fast-growing broiler chickens and causes economic loss. Despite previous studies, the regulatory mechanism of TD remains unclear thought to be primarily based on thiram induction, which may differ from naturally occurring diseases. To better understand TD, digital X-ray machine was used in present study determine its incidence four hundred yellow-feathered chickens. The results showed 22% after 6 weeks gradually decreased 8 10 weeks. body weight broilers with significantly compared NTD broilers. In addition, length density tibia were reduced eight weeks, This also examined tibial quality parameters (n = 12) found mineral content, density, ash calcium phosphorus content Transcriptome analysis revealed 849 differentially expressed genes (DEGs) growth plate between 6) groups 6). These enriched ECM-receptor interaction, cytokine-cytokine receptor signaling pathway, TGF-β signaling. Genes encoding alpha chain type XII collagen, is,

Language: Английский

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Plasma exosome-derived miR-455-5p targets RPS6KB1 to regulate cartilage homeostasis in valgus-varus deformity (Gallus gallus)

Poultry Science, Journal Year: 2024, Volume and Issue: 103(11), P. 104169 - 104169

Published: Aug. 11, 2024

Valgus-varus deformity (VVD) is a common long bone in broilers. Imbalance cartilage homeostasis the main feature of leg disease. Exosomes act as an important intercellular communication vector that regulates chondrogenesis by encapsulating specific nucleic acids and proteins. However, exact mechanism how plasma exosomal miRNAs regulate VVD broilers remains unclear. This study first demonstrated structural disorder, growth retardation, reduced proliferative capacity vitro vivo. Subsequently, Normal broiler exosomes were collected for miRNA sequencing. Cartilage-specific miR-455-5p was extraordinarily emphasized performing bioinformatics analysis on differential target genes further validated tissue expression profiling. PKH67 fluorescently labeled shown to be taken up chondrocytes, deliver miR-455-5p, inhibit chondrocyte proliferation, disrupt their homeostasis, these effects could inhibited miR-inhibitors. Mechanistically, MiR-455-5p targets Ribosomal Protein S6 Kinase B1 (RPS6KB1) RPS6 phosphorylation reduce synthesis key proteins which turn inhibits proliferation disrupts its homeostasis. In conclusion, present identified abnormalities clarified exosome-derived

Language: Английский

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