Electrochemical enzyme biosensor based on gold nanoparticles/polyaniline composites for highly specific rapidly detection of chlorpyrifos residues in traditional Chinese medicines DOI Creative Commons
Bolu Sun, Ying Lv,

Quhuan Ma

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 10, 2024

Abstract Chlorpyrifos (CPF) is an insecticide and acaricide that interrupts nerve conduction by reducing the activity of enzyme acetylcholinesterase. It can enter human body through a variety channels produce serious physiological effects. However, excessive use CPF in herbal production produces pesticide residues reduces yield high-quality herbs. Therefore, development effective convenient residue detection technology critical for monitoring as well danger avoidance. Based on this, electrochemical biosensor using acetylcholinesterase (AChE) recognition element was constructed highly sensitive rapid chlorpyrifos traditional Chinese medicine. The electrode substrate modification material polyaniline/gold nanoparticles (PANI/AuNPs) composite prepared PANI functional AuNPs with high conductivity large specific surface area, which has excellent electrical biocompatibility. Specifically, PANI/AuNPs offered exceptional active site AChE immobilization, greatly enhancing signals resulting from AChE-catalyzed iodination acetylthiocholine accelerating electron transfer. In contrast, AChE's better identification target analyte meant sensor selective organophosphorus residues. Under optimized conditions, showed good linearity range 1.00 × 10− 3 ~ 101 ppm limit 7.90 5 ppm. During practical sample testing, exhibited remarkable stability, reproducibility, sensitivity detecting medicines. This offers reliable tool precise propels progress enzyme-based biosensors, both fostering cultivation superior-quality medicines, enabling on-site detection.

Language: Английский

Semiconductor-based surface-enhanced Raman scattering sensing platforms: State of the art, applications and prospects in food safety DOI
Qinzhi Wang, Kuan‐Chang Chang, Qingli Yang

et al.

Trends in Food Science & Technology, Journal Year: 2024, Volume and Issue: 147, P. 104460 - 104460

Published: March 26, 2024

Language: Английский

Citations

26
Characterization of novel cold-active chitin deacetylase for green production of bioactive chitosan DOI Creative Commons
Mohamed N. Abd El-Ghany,

Salwa A.H. Hamdi,

Ahmed K. Zahran

et al.

AMB Express, Journal Year: 2025, Volume and Issue: 15(1)

Published: Jan. 4, 2025

Abstract A Novel cold-active chitin deacetylase from Shewanella psychrophila WP2 (SpsCDA) was overexpressed in Escherichia coli BL21 and employed for deacetylation of to chitosan. The produced chitosan characterized, its antifungal activity investigated against Fusarium oxysporum . purified recombinant SpsCDA appeared as a single band on SDS-PAGE at approximately 60 kDa, specific 92 U/mg. optimum temperature pH were 15 °C 8.0, respectively, the enzyme significantly enhanced presence NaCl. bioconversion by accomplished 72 h, yield 69.2%. solubility estimated be 73.4%, degree 78.1%. molecular weight 224.7 ± 8.4 kDa with crystallinity index (CrI) value 18.75. Moreover, FTIR XRD spectra revealed characteristic peaks enzymatically compared standard chitosan, indicating their structural similarity. inhibited spore germination F. minimum inhibitory concentration (MIC) 1.56 mg/mL. potential effect is attributed inhibition accompanied ultrastructural damage membranes leakage cellular components, evidenced transmission electron microscopy (TEM), accumulation reactive oxygen species (ROS) that confirmed fluorescence microscopy. This study shed light S. provides candidate green preparation

Language: Английский

Citations

2
ROS acted as an initial role in selenium nanoparticles alleviating insecticide chlorpyrifos-induced oxidative stress, pyroptosis, and intestinal barrier dysfunction in porcine intestinal epithelial cells DOI

Minna Qiu,

Zhiyu Hao,

Yuhao Liu

et al.

Pesticide Biochemistry and Physiology, Journal Year: 2025, Volume and Issue: 211, P. 106418 - 106418

Published: April 16, 2025

Language: Английский

Citations

2
Detection of organophosphorus pesticides by a fluorescent sensing assay coupled with enzyme inhibition DOI
Yi Ma,

Xiaofang Liu,

Jia Zheng

et al.

Journal of Food Composition and Analysis, Journal Year: 2024, Volume and Issue: 129, P. 106139 - 106139

Published: March 1, 2024

Language: Английский

Citations

9
Exposure to the environmental pollutant chlorpyrifos induces hepatic toxicity through activation of the JAK/STAT and MAPK pathways DOI

Huiyang Fu,

Yao Ge, Xiyuan Liu

et al.

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 928, P. 171711 - 171711

Published: March 16, 2024

Language: Английский

Citations

7
Advances in Microfluidics Techniques for Rapid Detection of Pesticide Residues in Food DOI Creative Commons

Zhuoao Jiang,

Zhuang Yu,

Shentian Guo

et al.

Foods, Journal Year: 2023, Volume and Issue: 12(15), P. 2868 - 2868

Published: July 28, 2023

Food safety is a significant issue that affects people worldwide and tied to their lives health. The of pesticide residues in food just one many issues related safety, which leave crops are transferred through the chain human consumption. Foods contaminated with pose serious risk health, including carcinogenicity, neurotoxicity, endocrine disruption. Although traditional methods, gas chromatography, high-performance liquid mass spectrometry, can be used achieve quantitative analysis residues, disadvantages these techniques, such as being time-consuming costly requiring specialist staff, limit application. Therefore, there need develop rapid, effective, sensitive equipment for food. Microfluidics rapidly emerging number fields due its outstanding strengths. This paper summarizes application microfluidic techniques pyrethroid, carbamate, organochlorine, organophosphate pesticides, well commercial products. Meanwhile, study also outlines development microfluidics combination 3D printing technology nanomaterials detecting

Language: Английский

Citations

11
The use of amino acids and their derivates to mitigate against pesticide-induced toxicity DOI Creative Commons
Guoping Zhao,

Wei-long Cheng,

Zhihui Zhang

et al.

Ecotoxicology and Environmental Safety, Journal Year: 2024, Volume and Issue: 276, P. 116340 - 116340

Published: April 17, 2024

Exposure to pesticides induces oxidative stress and deleterious effects on various tissues in non-target organisms. Numerous models investigating pesticide exposure have demonstrated metabolic disturbances such as imbalances amino acid levels within the organism. One potentially effective strategy mitigate toxicity involves dietary intervention by supplementing exogenous acids their derivates augment body's antioxidant capacity pesticide-induced harm, whose mechanism including bolstering glutathione synthesis, regulating arginine-NO metabolism, mitochondria-related stress, open of ion channels, well enhancing intestinal microecology. Enhancing synthesis through supplementation substrates N-acetylcysteine glycine is regarded a potent achieve this. Selection appropriate or for supplementation, determining an dosage, are utmost importance mitigation harm. More experimentation required that large population samples validate efficacy strategies, determine long-term low-dose exposure. This review provides insights guide future research aimed at preventing alleviating derivates.

Language: Английский

Citations

3
Chitosan-oligosaccharide alleviates chlorpyrifos-induced biochemical and developmental toxicity and reduces its accumulation in wheat (Triticum aestivum L.) DOI Creative Commons

Jingchong Li,

Wende Zheng,

Jingkun Li

et al.

Ecotoxicology and Environmental Safety, Journal Year: 2025, Volume and Issue: 295, P. 118146 - 118146

Published: April 1, 2025

Language: Английский

Citations

0
Sanghuangporus vaninii polysaccharides alleviates the effects of chlorpyrifos exposure on mouse liver: role of the gut- liver axis DOI

Jinze Liu,

Li Sun, Y. Tang

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: 315, P. 144504 - 144504

Published: May 21, 2025

Language: Английский

Citations

0
Nir-derived thermal field inhomogeneity in attapulgite-based particles for smart and precise pesticide delivery DOI

Xueqi Ma,

Chao‐Wen Chen, Guopeng Teng

et al.

Chemical Engineering Journal, Journal Year: 2025, Volume and Issue: unknown, P. 164287 - 164287

Published: May 1, 2025

Language: Английский

Citations

0