Electrochemical enzyme biosensor based on gold nanoparticles/polyaniline composites for highly specific rapidly detection of chlorpyrifos residues in traditional Chinese medicines
Abstract
Chlorpyrifos
(CPF)
is
an
insecticide
and
acaricide
that
interrupts
nerve
conduction
by
reducing
the
activity
of
enzyme
acetylcholinesterase.
It
can
enter
human
body
through
a
variety
channels
produce
serious
physiological
effects.
However,
excessive
use
CPF
in
herbal
production
produces
pesticide
residues
reduces
yield
high-quality
herbs.
Therefore,
development
effective
convenient
residue
detection
technology
critical
for
monitoring
as
well
danger
avoidance.
Based
on
this,
electrochemical
biosensor
using
acetylcholinesterase
(AChE)
recognition
element
was
constructed
highly
sensitive
rapid
chlorpyrifos
traditional
Chinese
medicine.
The
electrode
substrate
modification
material
polyaniline/gold
nanoparticles
(PANI/AuNPs)
composite
prepared
PANI
functional
AuNPs
with
high
conductivity
large
specific
surface
area,
which
has
excellent
electrical
biocompatibility.
Specifically,
PANI/AuNPs
offered
exceptional
active
site
AChE
immobilization,
greatly
enhancing
signals
resulting
from
AChE-catalyzed
iodination
acetylthiocholine
accelerating
electron
transfer.
In
contrast,
AChE's
better
identification
target
analyte
meant
sensor
selective
organophosphorus
residues.
Under
optimized
conditions,
showed
good
linearity
range
1.00
×
10−
3
~
101
ppm
limit
7.90
5
ppm.
During
practical
sample
testing,
exhibited
remarkable
stability,
reproducibility,
sensitivity
detecting
medicines.
This
offers
reliable
tool
precise
propels
progress
enzyme-based
biosensors,
both
fostering
cultivation
superior-quality
medicines,
enabling
on-site
detection.
Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown
Published: Dec. 10, 2024
Language: Английский