Dissolved
black
carbon
(DBC)
is
known
for
strong
environmental
mobility
and
photodegradation
to
contaminants.
But
studies
on
the
impact
of
metal
ions
nitrogen-doped
DBC
(NDBC)
antibiotics
are
barely
found.
Herein,
performance
tetracycline
(TC)
induced
by
NDBC
involving
Cr
(VI)
or
Fe
(III)
was
explored.
Adding
reduces
TC.
Detailedly,
efficiency
TC
79%,
which
decreases
37%
when
exists,
that
slightly
53%
exists.
Possible
reasons
has
a
higher
complexation
ability
with
than
according
FL
EEMs
spectra
NDBC,
leads
reduction
major
reactive
species
(RS)
(3NDBC*
1O2)
during
Finally,
adding
formation
rate
3NDBC*
1O2
more
markedly
(III).
This
paper
provides
deeper
insight
into
fate
DBC,
ions,
in
water
body.
Charosphere,
a
highly
active
zone
between
biochar
and
surrounding
soil,
is
widely
present
in
agricultural
wildfire-affected
soils,
yet
whether
reactive
oxygen
species
(ROS)
are
produced
within
the
charosphere
remains
unclear.
Herein,
production
spatiotemporal
evolution
of
ROS
were
explored.
In
situ
capture
visualized
gradual
decrease
with
increasing
distance
from
biochar/soil
interface.
Temporally,
O2•-
H2O2
contents
initially
increased
then
declined
incubation
time,
peaking
at
3.04
5.40
μmol
kg-1,
respectively,
while
•OH
content
decreased
continuously.
High-throughput
sequencing
revealed
that
dissolved
(DBC)
facilitated
by
promoting
growth
bacteria
electron-releasing
capacity,
such
as
Bacteroidetes,
Acidobacteria,
Actinobacteria,
Chloroflexi.
Additionally,
adding
electron
transfer-weakened
DBC
significantly
(ANOVA,
P
<
0.05),
demonstrating
also
served
shuttle
electron-storing
materials
to
promote
accelerating
transfer.
This
was
further
confirmed
via
fluorescence
imaging,
which
visually
showed
stronger
transfer
ability
near
soil/biochar
surface.
Inhibition
isotope
experiments
critical
role
CO2
emissions,
primarily
soil
organic
carbon.
study
highlights
prevalent
overlooked
hotspot,
advancing
our
understanding
carbon
turnover
soils.
