Multi-omics insights into the response ofAspergillus parasiticusto long-chain alkanes in relation to polyethylene degradation DOI Creative Commons
Romanos Siaperas, George Taxeidis, Efstratios Nikolaivits

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 16, 2024

Abstract Plastic pollution presents a global challenge, with polyethylene (PE) being among the most persistent plastics due to its durability and environmental resilience. In this study, we employed multi-omics approach study ability of Aspergillus parasiticus MM36, an isolate derived from Tenebrio molitor intestines, metabolize long-chain alkanes (lcAlk) secrete enzymes able modify PE. The fungus was grown hexadecane (C16) or mixture lcAlk (C24 C36) as carbon sources culture supernatants were tested daily for their Proteomic analysis identified induced oxidases potentially involved in PE functionalization. Key include multicopper oxidases, peroxidases, unspecific peroxygenase FAD-dependent monooxygenases. Surfactant proteins facilitating enzymatic cellular interaction hydrophobic lcAlk, such one hydrophobin, three surface-binding (HsbA) cerato platanin, present all secretomes. Transcriptomic comparing C16 cultures highlighted enrichment oxidoreductase activities carboxylic acid metabolism both incubation days, transmembrane transporters transferases predominating on day 2 biosynthetic processes 3. cultures, hydrolytic enzymes, including esterases, upregulated alongside Baeyer-Villiger monooxygenases, suggesting shift toward sub-terminal hydroxylation. Integrating transcriptomic secretomic data, propose mechanism assimilation by A. involving extracellular oxyfunctionalization, hydrocarbon uptake via surface-modifying channeling through membrane energy consumption processes. This provides insights into fungal mechanisms alkane highlights relevance plastic degradation. Importance challenge marine life human health, Hydroxylation is regarded initial step degradation, similar oxidation, making alkane-degrading microbes promising source degraders. used investigate MM36 that secretomes key biosurfactants enable interact transform substrates like further revealed biological metabolism. By integrating these insights, highlight biodegradation. work advances our understanding contributions addressing pollution.

Language: Английский

Targeted Genome Mining Facilitates the Discovery of a Promiscuous, Hyperthermostable Amidase from Thermovenabulum gondwanense with Notable Nylon‐Degrading Capacity DOI Creative Commons

Esther R. Hoffman,

Alana M. M. Rangaswamy, Maria E. Cleveland

et al.

Angewandte Chemie International Edition, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 23, 2024

Abstract Plastics are ubiquitous in our ecosystems, and microplastic accumulation the environment is an emerging global health concern. Since available recycling technologies not economically competitive with primary plastic production, use expected to reach 1231 megatons by 2060, 493 leeching into each year. To identify new nylon‐recycling biotechnologies, targeted genome mining was used thermostable enzymes capable of degrading polyamides. Here, we describe characterization a novel protein sourced from Thermovenabulum gondwanense : TvgC. TvgC extremely stable, exhibiting melting temperature 93 °C no detectable losses hydrolytic activity after one week at 60 °C. While nylonases primarily process nylon‐6, catalysed degradation both nylon‐6 nylon‐6,6 films, which considerably more difficult degrade. Finally, conversion experiments demonstrate that achieves 1.2 wt % film, comparable most highly engineered nylonases. This hyperthermostable represents excellent starting point for future engineering increasingly efficient

Language: Английский

Citations

0
Targeted Genome Mining Facilitates the Discovery of a Promiscuous, Hyperthermostable Amidase from Thermovenabulum gondwanense with Notable Nylon‐Degrading Capacity DOI Creative Commons

Esther R. Hoffman,

Alana M. M. Rangaswamy, Maria E. Cleveland

et al.

Angewandte Chemie, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 23, 2024

Abstract Plastics are ubiquitous in our ecosystems, and microplastic accumulation the environment is an emerging global health concern. Since available recycling technologies not economically competitive with primary plastic production, use expected to reach 1231 megatons by 2060, 493 leeching into each year. To identify new nylon‐recycling biotechnologies, targeted genome mining was used thermostable enzymes capable of degrading polyamides. Here, we describe characterization a novel protein sourced from Thermovenabulum gondwanense : TvgC. TvgC extremely stable, exhibiting melting temperature 93 °C no detectable losses hydrolytic activity after one week at 60 °C. While nylonases primarily process nylon‐6, catalysed degradation both nylon‐6 nylon‐6,6 films, which considerably more difficult degrade. Finally, conversion experiments demonstrate that achieves 1.2 wt % film, comparable most highly engineered nylonases. This hyperthermostable represents excellent starting point for future engineering increasingly efficient

Language: Английский

Citations

0
Chemical Reactivity of Weathered Nanoplastics and Their Interactions with Heavy Metals DOI
Yingnan Huang, Fei Dang, Yujun Wang

et al.

Environmental Science Nano, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

Nanoplastics (NPs) are chemically reactive following abiotic and biotic weathering processes. These weathered NPs have the potential to facilitate transformation of legacy contaminants, such as heavy metals.

Language: Английский

Citations

0
Genetically encoded biosensors for the circular plastics bioeconomy DOI Creative Commons
Micaela Chacón, Neil Dixon

Metabolic Engineering Communications, Journal Year: 2024, Volume and Issue: 19, P. e00255 - e00255

Published: Nov. 28, 2024

Language: Английский

Citations

0
Multi-omics insights into the response ofAspergillus parasiticusto long-chain alkanes in relation to polyethylene degradation DOI Creative Commons
Romanos Siaperas, George Taxeidis, Efstratios Nikolaivits

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 16, 2024

Abstract Plastic pollution presents a global challenge, with polyethylene (PE) being among the most persistent plastics due to its durability and environmental resilience. In this study, we employed multi-omics approach study ability of Aspergillus parasiticus MM36, an isolate derived from Tenebrio molitor intestines, metabolize long-chain alkanes (lcAlk) secrete enzymes able modify PE. The fungus was grown hexadecane (C16) or mixture lcAlk (C24 C36) as carbon sources culture supernatants were tested daily for their Proteomic analysis identified induced oxidases potentially involved in PE functionalization. Key include multicopper oxidases, peroxidases, unspecific peroxygenase FAD-dependent monooxygenases. Surfactant proteins facilitating enzymatic cellular interaction hydrophobic lcAlk, such one hydrophobin, three surface-binding (HsbA) cerato platanin, present all secretomes. Transcriptomic comparing C16 cultures highlighted enrichment oxidoreductase activities carboxylic acid metabolism both incubation days, transmembrane transporters transferases predominating on day 2 biosynthetic processes 3. cultures, hydrolytic enzymes, including esterases, upregulated alongside Baeyer-Villiger monooxygenases, suggesting shift toward sub-terminal hydroxylation. Integrating transcriptomic secretomic data, propose mechanism assimilation by A. involving extracellular oxyfunctionalization, hydrocarbon uptake via surface-modifying channeling through membrane energy consumption processes. This provides insights into fungal mechanisms alkane highlights relevance plastic degradation. Importance challenge marine life human health, Hydroxylation is regarded initial step degradation, similar oxidation, making alkane-degrading microbes promising source degraders. used investigate MM36 that secretomes key biosurfactants enable interact transform substrates like further revealed biological metabolism. By integrating these insights, highlight biodegradation. work advances our understanding contributions addressing pollution.

Language: Английский

Citations

0