International journal of basic science in medicine,
Journal Year:
2023,
Volume and Issue:
8(4), P. 167 - 172
Published: Dec. 31, 2023
<strong
data-sider-select-id="80716161-719c-41bb-991b-7e7ff63fa34c">Introduction:
Uropathogenic
Escherichia
coli
(UPEC)
is
a
common
cause
of
urinary
tract
infections
(UTIs),
imposing
significant
healthcare
burden
worldwide.
Understanding
the
distribution
virulence
genes
among
UPEC
isolates
crucial
for
elucidating
pathogenesis
UTIs
and
developing
therapeutic
strategies.
data-sider-select-id="f8080be0-0e6c-4bf5-b018-4fe2ad5fe91a">Methods:
In
this
study,
total
100
previously
collected
from
UTI
patients
were
analyzed.
The
prevalence
various
associated
with
iron
acquisition,
including
siderophore
receptors
(ireA),
hemolysin
toxins
(hylB,
hylC,
hylD),
uptake
systems
(feoB,
fepC,
fyuA),
as
well
putative
transport
(modD,
prrA,
yc73),
was
assessed
using
Multiplex
PCR
method.
data-sider-select-id="4086c189-6e76-4275-b77c-8c5f33e74bbd">Results:
results
revealed
notable
variability
in
gene
prevalence,
fyuA
being
most
frequently
detected
63
(63%).
However,
hlyD
absent
all
isolates.
Other
such
feoB,
hlyB,
hlyC,
ireA,
modD,
yc73
exhibited
frequencies
ranging
9
(9%)
to
53
(53%).
Notably,
76
harboured
multiple
suggesting
their
potential
enhanced
pathogenicity
adaptation
host
environment.
data-sider-select-id="121bd187-b6aa-4d42-8e22-36b0316293bf">Conclusion:
diverse
underscores
dynamic
nature
strains
ability
adapt
environments.
Comprehending
these
patterns
offers
invaluable
insights
into
pathogenesis,
emphasizing
necessity
personalized
interventions.
There
pressing
need
additional
research
functional
significance
aiming
formulate
more
efficient
strategies
combat
UPEC-induced
UTIs.
Proceedings of the National Academy of Sciences,
Journal Year:
2024,
Volume and Issue:
121(45)
Published: Oct. 28, 2024
Microplastic
is
globally
regarded
as
an
important
factor
impacting
biogeochemical
cycles,
yet
our
understanding
of
such
influences
limited
by
the
uncertainties
intricate
microbial
processes.
By
multiomics
analysis,
coupled
with
soil
chemodiversity
characterization
and
carbon
use
efficiency
(CUE),
we
investigated
how
responses
to
microplastics
impacted
cycling
in
a
long-term
field
experiment.
We
showed
that
biodegradable
promoted
organic
accrual
average
2.47%,
while
nondegradable
inhibited
it
17.4%,
consequence
virus–bacteria
coadaptations
disturbance.
In
relevant
functional
pathways,
significantly
(
P
<
0.05)
enhanced
abundance
transcriptional
activity
related
complex
carbohydrate
metabolism,
whereas
functions
involved
amino
acid
metabolism
glycolysis.
Accordingly,
viral
lysis
treatments
introduce
more
compounds
dissolved
matters,
thus
benefiting
oligotrophs
high
metabolic
capabilities
exploitation
competition.
contrast,
enriched
auxiliary
genes
through
“piggyback-the-winner”
strategy,
conferring
dominant
copiotrophs,
substrate
utilization
capabilities.
These
virus–host
interactions
were
also
demonstrated
corresponding
plastisphere,
which
would
alter
resource
allocation
via
CUE,
affecting
storage
consequently.
Overall,
results
underscore
importance
viral–host
microplastics-dependent
ecosystem.
Annals of Clinical Microbiology and Antimicrobials,
Journal Year:
2024,
Volume and Issue:
23(1)
Published: April 10, 2024
Elizabethkingia
is
emerging
as
an
opportunistic
pathogen
in
humans.
The
aim
of
this
study
was
to
investigate
the
clinical
epidemiology,
antimicrobial
susceptibility,
virulence
factors,
and
genome
features
spp.
While
substantial
amounts
of
antibiotics
and
pesticides
are
applied
to
maintain
orchard
yields,
their
influence
on
the
dissemination
risk
antibiotic
resisitome
in
food
chain
remains
poorly
understood.
In
this
study,
we
characterized
bacterial
fungal
communities
differentiated
both
resistance
genes
(ARGs)
virulence
factor
(VFGs)
soil,
Chinese
bayberry
(matured
fallen),
fruit
fly
gut,
collected
from
five
geographic
locations.
Our
results
showed
that
guts
soils
exhibit
a
higher
abundance
ARGs
VFGs
compared
with
fruits.
We
identified
112
shared
75
VFGs,
aminoglycoside
adherence
being
among
most
abundant.
The
co-occurrence
network
revealed
some
microbes,
such
as
Polycyclic
aromatic
hydrocarbons
(PAHs)
are
toxic
and
persistent
pollutants
that
widely
distributed
in
the
environment.
PAHs
to
microorganisms
pose
ecological
risks.
Bacteria
encode
enzymes
for
PAH
degradation
through
specific
genes,
thereby
mitigating
pollution.
However,
due
PAHs'
complexity,
information
on
global
potential,
diversity,
associated
risks
of
PAH-degrading
microbes
soils
is
lacking.
In
this
study,
we
analyzed
121
genes
selected
33
as
marker
predict
potential
within
soil
microbiome.
By
constructing
a
Hidden
Markov
Model,
identified
4990
species
carrying
40,039
metagenomic
assembly
genomes,
with
Burkholderiaceae
Stellaceae
emerging
high-potential
degraders.
We
demonstrated
candidate
degraders
predominantly
emerged
artificial
farmland,
significantly
fewer
present
extreme
environments,
driven
by
factors
such
average
annual
rainfall,
organic
carbon,
human
modification
terrestrial
systems.
Furthermore,
comprehensively
quantified
each
host
future
practical
applications
using
three
indicators
(antibiotic
resistance
virulence
factors,
pathogenic
bacteria).
found
degrader
has
significant
application
prospects.
Our
research
will
help
determine
biosynthetic
globally
further
identify
bacteria
at
lower
risk.
Frontiers in Microbiology,
Journal Year:
2025,
Volume and Issue:
16
Published: May 1, 2025
Sustainable
organic
management
practices
have
gained
significant
attentions
for
its
potential
health
and
environmental
benefits.
However,
the
spread
of
antibiotic
resistance
genes
(ARGs)
virulence
factors
(VFs)
in
soils,
plants,
agricultural
products
has
severely
limited
development
managements
on
agriculture.
At
present,
distribution
assembly
ARGs
VFs
managed
tea
plantation
systems
remains
largely
unknown.
Here,
we
used
metagenomic
analysis
to
explore
soil
microbial
taxa,
20
years
conventional
(CM)
(OM)
soils.
Results
showed
that
total
abundance
OM
was
16.9%
(
p
<
0.001)
higher
than
CM,
increased
were
rpoB2
,
evgS
MuxB
TaeA
efrA
.
As
VFs,
significantly
adherence,
stress
protein
actin-based
motility
compared
CM.
Moreover,
relative
taxa
harboring
which
Streptomyces
Pseudomonas
Terrabacter
Network
suggested
positive
interactions
taxa-ARGs,
taxa-VFs
ARGs-VFs
Impact
stochastic
process
stronger
Overall,
these
findings
provide
a
basis
integrating
ARGs,
pathogen
hosts
assess
ecological
risks
long-term
efforts
need
be
done
reducing
bacterial
pathogens
fertilizers
