Benzimidazole Fungicide Carbendazim Induces Gut Inflammation through the TLR5/NF-κB Pathway in Grass Carp

Environmental Science & Technology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 2, 2025

Fungicides have been increasingly used across various sectors, including agriculture and textiles. The biocidal properties of fungicides may negatively impact the stability intestinal microbiota, thereby posing a threat to health. In this study, we investigated mechanisms damage functional abnormalities in grass carp following 42-day exposure widely fungicide carbendazim at environmentally relevant concentrations (0.2 20 μg/L). Histopathological observations, mRNA protein expression analyses, biochemical analysis, quantification short-chain fatty acids (SCFAs), cytokines, lipopolysaccharide (LPS), 16S ribosomal ribonucleic acid (rRNA), as well internal transcribed spacer (ITS) sequencing, were performed. At concentrations, strongly induced inflammation, leading increased transcriptional translational levels genes involved toll-like receptor five (TLR5)/nuclear factor kappa B (NF-κB) pathway, TLR5, NF-κB, interleukin-1 beta (IL-1β), tumor necrosis factor-alpha (TNFα). Additionally, damaged barriers reduced tight junction proteins (e.g., occludin zonula occludens-1/2), goblet cells, immunoglobulin M levels, while also disrupting gut microbiome, metabolic disorders, particularly decreases SCFAs increases LPS. Treatment with TLR5 antagonist TH1020 mitigated inflammation caused by carbendazim, subsequently improving mechanical barrier function. Overall, our findings provide new insights into toxicological underlying carp, indicating that poses significant nontarget organisms. Given its widespread detection environment, these results underscore substantial ecological risks health fish living carbendazim-contaminated water bodies.

Language: Английский

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The azole biocide climbazole induces oxidative stress, inflammation, and apoptosis in fish gut

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 923, P. 171475 - 171475

Published: March 6, 2024

Language: Английский

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Aquatic Microbiomes Under Stress: The Role of Gut Microbiota in Detoxification and Adaptation to Environmental Exposures

Journal of Hazardous Materials Advances, Journal Year: 2025, Volume and Issue: unknown, P. 100612 - 100612

Published: Jan. 1, 2025

Language: Английский

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Neurotoxicity and intestinal microbiota dysbiosis induced by per- and polyfluoroalkyl substances in crucian carp (Carassius auratus)

Journal of Hazardous Materials, Journal Year: 2024, Volume and Issue: 478, P. 135611 - 135611

Published: Aug. 23, 2024

Language: Английский

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Toxicological effects of chemical pesticides in fish: Focusing on intestinal injury and gut microbial dysbiosis

Pesticide Biochemistry and Physiology, Journal Year: 2025, Volume and Issue: 211, P. 106405 - 106405

Published: April 9, 2025

Language: Английский

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Effects of Vibrio harveyi and oligochitosan non-specific immune response of hybrid groupers (Epinephelus fuscoguttatus ♀ × Epinephelus lanceolatus ♂) supplementation

Aquaculture International, Journal Year: 2025, Volume and Issue: 33(4)

Published: April 24, 2025

Language: Английский

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Assessing the chronic toxicity of climbazole to Daphnia magna: Physiological, biochemical, molecular, and reproductive perspectives

Comparative Biochemistry and Physiology Part C Toxicology & Pharmacology, Journal Year: 2024, Volume and Issue: 287, P. 110061 - 110061

Published: Oct. 20, 2024

Language: Английский

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