Generation of in vivo-like multicellular liver organoids by mimicking developmental processes: A review

Regenerative Therapy, Journal Year: 2024, Volume and Issue: 26, P. 219 - 234

Published: June 1, 2024

Liver is involved in metabolic reactions, ammonia detoxification, and immunity. Multicellular liver tissue cultures are more desirable for drug screening, disease modeling, researching transplantation therapy, than hepatocytes monocultures. Hepatocytes monocultures not stable long. Further, hepatocyte-like cells induced from pluripotent stem vivo functionally dissimilar. Organoid technology circumvents these issues by generating functional ex intrinsic progenitor extrinsic cells, including cells. To function as tissue, the organoid must be arranged precisely 3-dimensional space, closely mimicking tissue. Moreover, long term functioning, organoids appropriately vascularized contact with neighboring epithelial tissues (e.g., bile canaliculi intrahepatic duct, or extrahepatic ducts). Recent discoveries developmental biology allows one to successfully induce component generate organoids. Thus, here, this review, we summarize current state of knowledge on development a focus its application different We also cover future prospects creating (functionally structurally) vivo-like using development.

Language: Английский

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Planar cell polarity is crucial for proper morphogenesis of the bile ducts

Journal of Hepatology, Journal Year: 2024, Volume and Issue: 81(1), P. 17 - 19

Published: March 26, 2024

Language: Английский

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