PRSS3/mesotrypsin as a putative regulator of the biophysical characteristics of epidermal keratinocytes in superficial layers DOI Creative Commons

M. Kida,

Junya Abe,

Haruna Hori

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: May 29, 2024

Abstract Mesotrypsin, encoded by the PRSS3 gene, is a distinctive trypsin isoform renowned for its exceptional resistance to traditional inhibitors and unique substrate specificity. Within skin epidermis, this protein primarily expresses in upper layers of stratified epidermis plays crucial role processing pro-filaggrin (Pro-FLG). Although prior studies have partially elucidated functions using primary cultured keratinocytes, challenges persist due these cells' differentiation-activated cell death program. In present study, HaCaT characterized minimal endogenous mesotrypsin expression sustained proliferation differentiated states, were utilized further scrutinize function mesotrypsin. Despite ready degradation intact form active cells, fusion with Venus, flanked peptide linker, enables evasion from elimination machinery, thus facilitating activation Pro-FLG system. Inducing Venus-mesotrypsin cells resulted flattened phenotype reduced proliferative capacity. Moreover, displayed altered F-actin assembly, enhanced E-cadherin adhesive activity, facilitated tight junction formation without overtly influencing epidermal differentiation. These findings underscore mesotrypsin's potentially pivotal shaping characteristic cellular morphology layers.

Language: Английский

Autophagy-Mediated Cellular Remodeling during Terminal Differentiation of Keratinocytes in the Epidermis and Skin Appendages DOI Creative Commons
Leopold Eckhart, Флориан Грубер, Supawadee Sukseree

et al.

Cells, Journal Year: 2024, Volume and Issue: 13(20), P. 1675 - 1675

Published: Oct. 10, 2024

The epidermis of the skin and appendages, such as nails, hair sebaceous glands, depend on a balance cell proliferation terminal differentiation in order to fulfill their functions at interface body environment. epithelial cells skin, commonly referred keratinocytes, involves major remodeling processes that generate metabolically inactive remnants serving building blocks epidermal stratum corneum, nail plates shafts. Only gland results disintegration holocrine secretion. A series studies performed past decade have revealed lysosome-dependent intracellular degradation mechanism autophagy is active during keratinocyte differentiation, blockade significantly alters properties products. Here, we present model for autophagy-mediated organelles cytosolic proteins an important contributor cellular differentiation. roles are discussed comparison alternative mechanisms context programmed death integral end point

Language: Английский

Citations

3
Cell differentiation in the embryonic periderm and in scaffolding epithelia of skin appendages DOI Creative Commons
Leopold Eckhart, Karin Brigit Holthaus, Attila Placido Sachslehner

et al.

Developmental Biology, Journal Year: 2024, Volume and Issue: 515, P. 60 - 66

Published: July 2, 2024

Terminal differentiation of epithelial cells is critical for the barrier function skin, growth skin appendages, such as hair and nails, development amniotes. Here, we present hypothesis that in embryonic periderm shares characteristic features with support morphogenesis cornified appendages during postnatal life. The prevents aberrant fusion adjacent sites early development. It shed off when keratinocytes epidermis form layer, stratum corneum. A similar role played by epithelia ensheath cornifying until they disintegrate to allow separation mature part appendage from tissue. These epithelia, exemplified inner root sheath follicles close free edge nails or claws, are referred scaffolding epithelia. regard their transient functions separating tissues conserved expression trichohyalin trichohyalin-like genes mammals birds. Thus, propose parts peridermal program were coopted a new evolution

Language: Английский

Citations

1
The human retroviral-like aspartic protease 1 (ASPRV1): from in vitro studies to clinical correlations DOI Creative Commons
János András Mótyán, József Tőzsér

Journal of Biological Chemistry, Journal Year: 2024, Volume and Issue: 300(9), P. 107634 - 107634

Published: Aug. 3, 2024

Language: Английский

Citations

1
PRSS3/mesotrypsin as a putative regulator of the biophysical characteristics of epidermal keratinocytes in superficial layers DOI Creative Commons

M. Kida,

Junya Abe,

Haruna Hori

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: May 29, 2024

Abstract Mesotrypsin, encoded by the PRSS3 gene, is a distinctive trypsin isoform renowned for its exceptional resistance to traditional inhibitors and unique substrate specificity. Within skin epidermis, this protein primarily expresses in upper layers of stratified epidermis plays crucial role processing pro-filaggrin (Pro-FLG). Although prior studies have partially elucidated functions using primary cultured keratinocytes, challenges persist due these cells' differentiation-activated cell death program. In present study, HaCaT characterized minimal endogenous mesotrypsin expression sustained proliferation differentiated states, were utilized further scrutinize function mesotrypsin. Despite ready degradation intact form active cells, fusion with Venus, flanked peptide linker, enables evasion from elimination machinery, thus facilitating activation Pro-FLG system. Inducing Venus-mesotrypsin cells resulted flattened phenotype reduced proliferative capacity. Moreover, displayed altered F-actin assembly, enhanced E-cadherin adhesive activity, facilitated tight junction formation without overtly influencing epidermal differentiation. These findings underscore mesotrypsin's potentially pivotal shaping characteristic cellular morphology layers.

Language: Английский

Citations

0