Lactobacillus rhamnosus GG-derived extracellular vesicles promote wound healing via miR-21-5p-mediated re-epithelization and angiogenesis DOI Creative Commons
Juan Wang,

Xiaojie Li,

Xinyue Zhao

et al.

Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)

Published: Oct. 19, 2024

Extracellular vesicles (EVs), especially those derived from stem cells, have emerged as a novel treatment for promoting wound healing in regenerative medicine. However, the clinical application of mammalian cells-derived EVs is hindered by their high cost and low yields. Inspired ability to mediate interkingdom communication, we explored therapeutic potential released probiotic strain Lactobacillus rhamnosus GG (LGG) skin elucidated underlying mechanism involved. Using full-thickness wound-healing mouse models, found that LGG-EVs accelerated procedures, including increased re-epithelialization promoted angiogenesis. vitro experiments, further demonstrated boosted proliferation migration capacities both epithelial endothelial well tube formation. miRNA profiling analysis revealed miR-21-5p was highly enriched LGG-EV significantly level recipient cells. Mechanically, induced regulatory effects via mediated metabolic signaling rewiring. Our results suggest LGG could serve promising candidate accelerating possibly treating chronic impaired conditions.

Language: Английский

Advancements in Regenerative Medicine for Aesthetic Dermatology: A Comprehensive Review and Future Trends DOI Creative Commons
Federica Trovato,

Stefano Ceccarelli,

Simone Michelini

et al.

Cosmetics, Journal Year: 2024, Volume and Issue: 11(2), P. 49 - 49

Published: March 31, 2024

The growing interest in maintaining a youthful appearance has encouraged an accelerated development of innovative, minimally invasive aesthetic treatments for facial rejuvenation and regeneration. close correlation between tissue repair, regeneration, aging paved the way application regenerative medicine principles cosmetic dermatology. theoretical substrates applications dermo-aesthetics are plentiful. However, dermatology is emerging field needs more data vivo trials to reach consensus on standardization methods. In this review, we summarize techniques as they apply dermatology, suggesting unexplored fields future directions.

Language: Английский

Citations

5
Alloferon and IL-22 receptor expression regulation on the pathogenesis of imiquimod-induced psoriasis DOI Creative Commons
Tomoyo Agura, Hyejung Jo, Seulgi Shin

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Feb. 24, 2025

Language: Английский

Citations

0
The acid sphingomyelinase inhibitor imipramine enhances the release of UV photoproduct‐containing DNA in small extracellular vesicles in UVB‐irradiated human skin DOI Creative Commons
M. Alexandra Carpenter,

Anita Thyagarajan,

Madison Owens

et al.

Photochemistry and Photobiology, Journal Year: 2024, Volume and Issue: 100(6), P. 1894 - 1901

Published: March 3, 2024

Nucleic acids, lipids, and other cell components can be found within different types of extracellular vesicles (EVs), which include apoptotic bodies (ABs), large (LEVs), small (SEVs). Release LEVs from cells reduced by genetic or pharmacological inhibition the enzyme acid sphinogomyelinase (aSMase), indeed several studies have demonstrated a role for clinically approved aSMase inhibitor imipramine in blocking LEV release, including response to UVB exposure. Given that exposure keratinocytes radiation results generation UVR photoproducts DNA subsequently association with ABs SEVs, we examined how impacts release containing at an early time point after Using model systems, cultured vitro, discarded human surgical skin ex vivo, biopsies obtained treated subjects, these pilot suggest treatment stimulates CPD-containing, SEV-associated DNA. These surprising findings indicate SEV pathways could linked UVB-irradiated may exacerbate systemic effects UVR-damaged throughout body.

Language: Английский

Citations

1
Lactobacillus rhamnosus GG-derived extracellular vesicles promote wound healing via miR-21-5p-mediated re-epithelization and angiogenesis DOI Creative Commons
Juan Wang,

Xiaojie Li,

Xinyue Zhao

et al.

Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)

Published: Oct. 19, 2024

Extracellular vesicles (EVs), especially those derived from stem cells, have emerged as a novel treatment for promoting wound healing in regenerative medicine. However, the clinical application of mammalian cells-derived EVs is hindered by their high cost and low yields. Inspired ability to mediate interkingdom communication, we explored therapeutic potential released probiotic strain Lactobacillus rhamnosus GG (LGG) skin elucidated underlying mechanism involved. Using full-thickness wound-healing mouse models, found that LGG-EVs accelerated procedures, including increased re-epithelialization promoted angiogenesis. vitro experiments, further demonstrated boosted proliferation migration capacities both epithelial endothelial well tube formation. miRNA profiling analysis revealed miR-21-5p was highly enriched LGG-EV significantly level recipient cells. Mechanically, induced regulatory effects via mediated metabolic signaling rewiring. Our results suggest LGG could serve promising candidate accelerating possibly treating chronic impaired conditions.

Language: Английский

Citations

1