Fluconazole-induced changes in azole resistance and biofilm production in Candida glabratain vitro

Diagnostic Microbiology and Infectious Disease, Journal Year: 2025, Volume and Issue: 111(3), P. 116683 - 116683

Published: Jan. 9, 2025

Language: Английский

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Tetracycline and chloramphenicol exposure induce decreased susceptibility to tigecycline and genetic alterations in AcrAB-TolC efflux pump regulators in Escherichia coli and Klebsiella pneumoniae

PLoS ONE, Journal Year: 2025, Volume and Issue: 20(1), P. e0315847 - e0315847

Published: Jan. 22, 2025

Tigecycline (Tgc), a third-generation tetracycline is found as the last line of defense against multi-drug resistant bacteria. Recent increased rate resistance to tgc, human-restricted agent among animal bacteria poses significant global health challenge. Overuse first generation tetracyclines (Tet) and phenicols in animals have been suggested be associated with Tgc development. In current study we aimed determine effect chloramphenicol (Chl) overexposure on susceptibility. A Tet Chl-susceptible isolate K . pneumonia e E coli were exposed successively increasing concentrations separately until ≥4 times increase Chl MICs was observed. Susceptibility changes several antimicrobial agents tested using disk diffusion broth dilution methods. The genetic alterations genes coding for major AcrAB regulators including acrR (repressor acrAB ), ramR ramA soxR soxS ) pneumoniae lon (proteolytic degradation MarA), marR marA investigated. expression level acrB measured reverse transcription-quantitative polymerase chain reaction (RT-qPCR) method. excessive exposure (15 40 selection cycles) studied both antibiotics significantly decreased susceptibility Tet-resistant (R) Chl-R variants (n = 6) groups tigecycline (4–16 8–64 respectively) quinolones. About 58% 7) carried (frameshift mutations/locus deletion), MarR (L33R, A70T, G15S amino acid substitutions) Lon (L630F change, frameshift mutation) which upregulation. Our demonstrated capacity mutants revealed resistance/decreased mostly mediated by active efflux mechanism. Unaltered some strains indicates possible contribution other pumps or non-efflux-based mechanisms development multiple- antibiotic phenotype.

Language: Английский

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First report of MDR virulent Pseudomonas aeruginosa in apparently healthy Japanese quail (Coturnix japonica) in Bangladesh

PLoS ONE, Journal Year: 2025, Volume and Issue: 20(1), P. e0316667 - e0316667

Published: Jan. 24, 2025

Pseudomonas aeruginosa (P. aeruginosa) is a major pathogen associated conditions like septicaemia, respiratory disorders, and diarrhoea in poultry, particularly Japanese quail (Coturnix japonica). The infection causes huge economical losses due to its high transmissibility, mortality zoonotic potential. This study aimed isolate, identify, detect virulence genes, profile multidrug resistance (MDR) of P. from quail. Oral rectal swabs were collected 110 apparently healthy birds across various districts Bangladesh. Bacterial isolation identification performed using cultural, morphological, biochemical, polymerase chain reaction (PCR) methods. Antibiotic susceptibility was assessed the disc diffusion method, genes detected through PCR. Multivariable logistic regression used identify risk factors for infection. Both conventional PCR methods revealed that 25 (22.73%) positive aeruginosa. results showed Narsingdi five times more likely harbor species (OR: 5.02; 95% CI: 1.34-18.84) compared those Mymensingh Sadar. Additionally, younger than eight weeks had nearly six higher odds 5.93; 1.96-17.91) carrying older birds. Female almost four 3.77; 1.30-10.93) harboring males. All isolates exhibited multi drug-resistance patterns. Virulence gene analysis consistent presence exoA rhlR all isolates, while exoS, exoY, rhlI, rhlAB variable distribution. prevalence MDR virulent Dhaka divisions, highlights urgent need comprehensive 'One Health' approach proactively address mitigate potential this organism poses both human populations.

Language: Английский

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Mechanisms of Antimicrobial Resistance in Klebsiella: Advances in Detection Methods and Clinical Implications

Infection and Drug Resistance, Journal Year: 2025, Volume and Issue: Volume 18, P. 1339 - 1354

Published: March 1, 2025

Abstract: Klebsiella spp . are ubiquitous gram-negative bacteria, commonly present in natural environments and as part of the human microbiota. is involved occurrence development many diseases, effective antibiotics for it have attracted attention researchers. In recent years, its multi-drug resistance, particularly to carbapenems β-lactam antibiotics, has posed significant challenges clinical treatment. Therefore, a comprehensive understanding resistance mechanisms , along with enhancement detection methods, crucial effectively controlling spread drug-resistant strains guiding individualized This paper systematically reviews epidemiological characteristics, mechanisms, treatment strategies aiming provide new insights management this pathogen. Keywords: drug method, β-lactam,

Language: Английский

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A Critical Review of the Prospect of Integrating Artificial Intelligence in Infectious Disease Diagnosis and Prognosis

Interdisciplinary Perspectives on Infectious Diseases, Journal Year: 2025, Volume and Issue: 2025(1)

Published: Jan. 1, 2025

This paper explores the transformative potential of integrating artificial intelligence (AI) in diagnosis and prognosis infectious diseases. By analyzing diverse datasets, including clinical symptoms, laboratory results, imaging data, AI algorithms can significantly enhance early detection personalized treatment strategies. reviews how AI-driven models improve diagnostic accuracy, predict patient outcomes, contribute to effective disease management. It also addresses challenges ethical considerations associated with AI, data privacy, algorithmic bias, equitable access healthcare. Highlighting case studies recent advancements, underscores AI's role revolutionizing management its implications for future healthcare delivery.

Language: Английский

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The evolving epidemiology of antimicrobial resistance of ESKAPE pathogens isolated in the Intensive Care Unit of a Greek University Hospital

Diagnostic Microbiology and Infectious Disease, Journal Year: 2025, Volume and Issue: unknown, P. 116804 - 116804

Published: March 1, 2025

Language: Английский

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Molecular Typing of Bacterial Vaginosis Isolates by Using Enterobacterial Repetitive Intergenic Consensus (ERIC) PCR

Microbial Pathogenesis, Journal Year: 2025, Volume and Issue: unknown, P. 107564 - 107564

Published: April 1, 2025

Language: Английский

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Antibiotic susceptibility patterns of heavy metal-tolerant Gram-negative bacteria isolated from a major dumpsite in Ibadan, Nigeria

One Health Bulletin, Journal Year: 2025, Volume and Issue: unknown

Published: April 29, 2025

Objective: To determine the antibiotic susceptibility patterns of heavy metal-tolerant Gram-negative bacteria isolated from a major dumpsite in Ibadan, Nigeria. Methods: were using MacConkey and eosin methylene blue agar presumptive identification to species level was done conventional methods. The isolates screened for their tolerance metals. Antimicrobial tested by Kirby-Bauer disc diffusion method with following antibiotics: tetracycline (10 μg), cotrimoxazole (25 gentamicin cefuroxime (30 chloramphenicol ceftriaxone cefotaxime ciprofloxacin (5 amikacin ceftazidime meropenem μg). zones growth inhibition interpreted based on Clinical Laboratory Scientific Institute. Identification most 16S rRNA. Results: A total 79 isolated, 31 which tolerated minimum three metals (Pb at 10 350 μg/ mL; Cu 750 μg/mL; Cr μg/mL wet season Pb 2 450 300 dry season). All showed varying degrees multiple resistance indexes. highest (100%) least (51.6%). identified as Aeromonas hydrophila Pseudomonas aeruginosa . Conclusions: This study revealed that waste contains significant amounts antibiotic-resistant may pose serious public health risks if they enter food chain. highlighted untreated soils vegetable farming due pathogen metal transfer, it is unsuitable agricultural use.

Language: Английский

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Predicting Drug Resistance: The Use of Novel Inflammatory Markers in Identifying ESBL-Producing Klebsiella pneumoniae

Journal of Inflammation Research, Journal Year: 2025, Volume and Issue: Volume 18, P. 2153 - 2168

Published: Feb. 1, 2025

Purpose: To explore the association of Systemic Inflammatory Response Index (SIRI) and Immunity (SII) with extended-spectrum β-lactamase (ESBL)-producing Klebsiella pneumoniae (K. pneumoniae) its resistance prediction. Methods: A total 425 patients K. infections were included in study. Data on general clinical characteristics relevant laboratory indicators collected. The divided into ESBL-producing non-ESBL-producing groups based presence ESBLs. Logistic regression analysis was used to analyze risk factors associated . receiver operating characteristic (ROC) curve employed assess predictive efficacy SIRI SII for antibiotics. Results: levels group significantly higher than those group. showed that odds ratios 1.092 1.158, respectively, 95% confidence intervals 1.001– 1.115 1.015– 1.204, respectively. critical values predicting 1.067 579.68 SII, area under (AUC) 0.725 0.723, AUC piperacillin (PIP), amoxicillin/clavulanate (AMC), cefazolin (CZO) 0.614, 0.657, 0.648 SIRI, 0.675, 0.613, 0.625 Conclusion: are can be predict a patient's infection this organism. Additionally, accurately PIP, AMC, CZO Keywords: systemic inflammation response index, inflammatory immune β-lactamase, , prediction drug

Language: Английский

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A novel function of short cationic peptide FP-CATH9 without antimicrobial activity reverses resistance to minocycline in common multidrug-resistant gram-negative bacteria

Microbiology Spectrum, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 25, 2025

ABSTRACT The increase in bacterial resistance to minocycline and other tetracyclines poses a serious threat global public health. Because the development of new antibiotics has proven problematic, antibiotic sensitization therapy is now an effective coping strategy. While antimicrobial peptides generally exhibit broad-spectrum antibacterial activity good biocompatibility, naturally truncated portions (such as snake cathelicidin) often do not activity, their function remains unknown. FP-CATH9 short cationic peptide derived from FP-CATH (snake cathelicidin peptide) with amphiphilic α-helical structure no discernible activity. However, was previously found significantly enhance against gram-negative bacteria. In present paper, clinically relevant minocycline-resistant bacteria ( Escherichia coli , Klebsiella pneumoniae Acinetobacter baumannii Pseudomonas aeruginosa ) were used test for screening. be dose dependent double-dilution assay. synergistic on subsequently determined using checkerboard method. An ethidium bromide efflux revealed that caused accumulation Additionally, exhibited low hemolytic red blood cells cytotoxicity Raw264.7 cells. vivo model infection, combined 80% protective effect Galleria mellonella larvae infected multidrug-resistant K. . summary, adjuvant reverses by increasing intracellular minocycline. This finding broad application potential. IMPORTANCE existence pump system enables expel antibiotics, reduce concentration cells, make unable effectively inhibit or kill bacteria, which one main mechanisms antibiotics. some pumps are substrate specific, while others wide range substrates. this study, can specifically reverse provides way solve problem drug resistance.

Language: Английский

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