CryoCycle your grids: Plunge vitrifying and reusing clipped grids to advance cryoEM democratization DOI Creative Commons
Alex J. Noble, Viacheslav Serbynovskyi, Jing Wang

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: July 2, 2024

Abstract CryoEM democratization is hampered by access to costly plunge-freezing supplies. We introduce methods, called CryoCycle, for reliably blotting, vitrifying, and reusing clipped cryoEM grids. demonstrate that vitreous ice may be produced plunging grids with purified proteins into liquid ethane reused several times different protein samples. Furthermore, we the vitrification of thin areas cells prepared on gold-coated, pre-clipped

Language: Английский

Impact of SARS-CoV-2 Wuhan and Omicron Variant Proteins on Type I Interferon Response DOI Creative Commons
Marija Janevska,

Evelien Naessens,

Bruno Verhasselt

et al.

Viruses, Journal Year: 2025, Volume and Issue: 17(4), P. 569 - 569

Published: April 15, 2025

SARS-CoV-2 has demonstrated a remarkable capacity for immune evasion. While initial studies focused on the Wuhan variant and adaptive immunity, later emerging strains such as Omicron exhibit mutations that may alter their immune-modulatory properties. We performed comprehensive review of evasion mechanisms associated with viral proteins to focus evolutionary dynamics modulation. systematically analyzed compared impact all currently known type I interferon (IFN) responses using dual-luciferase reporter assay carrying an interferon-inducible promoter. Results revealed Nsp1, Nsp5, Nsp14, ORF6 are potent IFN inhibitors conserved across strains. Notably, we identified strain-specific differences, Nsp6 Spike exhibiting enhanced suppression in Omicron, whereas Envelope protein largely retained this function. To extend these findings, investigated selected primary human endothelial cells also observed differences response higher expressing strain variant, suggesting Omicron’s adaptational contribute damped course pandemic’s trajectory.

Language: Английский

Citations

0
CryoCycle your grids: Plunge vitrifying and reusing clipped grids to advance cryoEM democratization DOI Creative Commons
Viacheslav Serbynovskyi, Jing Wang, Eugene Chua

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 24, 2024

ABSTRACT CryoEM democratization is hampered by access to costly plunge-freezing supplies. We introduce methods, called CryoCycle, for reliably blotting, vitrifying, and reusing clipped cryoEM grids. demonstrate that vitreous ice may be produced plunging grids with purified proteins into liquid ethane reused several times different protein samples. Furthermore, we the vitrification of thin areas cells prepared on gold-coated, pre-clipped

Language: Английский

Citations

1
Selenium and Human Health DOI
Volkan Gelen, Adem Kara, Abdulsamed Kükürt

et al.

IntechOpen eBooks, Journal Year: 2023, Volume and Issue: unknown

Published: March 9, 2023

Selenium is a powerful trace element and antioxidant with multiple effects on metabolism human health. Available as dietary supplement for many years, it one of the most popular over-the-counter alternative medicines available. Written by researchers in field, this comprehensive book offers wide range expert reviews role selenium variety diseases well

Language: Английский

Citations

2
An Overview of the Antioxidant and Anti-Inflammatory Activity of Selenium DOI Creative Commons
Mehmet Başeğmez

IntechOpen eBooks, Journal Year: 2023, Volume and Issue: unknown

Published: May 24, 2023

Selenium, whose name comes from the Greek word for “Selene,” has been a topic of interest as micronutrient ever since it was described in 1817 by-product sulfuric acid manufacturing. most important both humans and animals, must be consumed daily to support body’s natural metabolism homeostasis. The small intestine is responsible absorption selenium its organic inorganic forms. Selenium then able widely distributed throughout diverse tissues, where plays an role regulation synthesis selenoproteins. human selenoproteins involves incorporation selenium-containing homolog cysteine each 25 proteins that make up this series. Many selenoproteins, including glutathione peroxidase (GPX), thioredoxin reductase (TrxR), iodothyronine deiodinases (IDD), function crucial cellular defenses against oxidative stress. Therefore, extremely boosting antioxidant defense. Recent studies have also shown there close relationship between inflammation, regulatory effects on inflammation by affecting expression various cytokines. This chapter’s goal thoroughly review research how related anti-inflammatory activity.

Language: Английский

Citations

2
CryoCycle your grids: Plunge vitrifying and reusing clipped grids to advance cryoEM democratization DOI Creative Commons
Alex J. Noble, Viacheslav Serbynovskyi, Jing Wang

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: July 2, 2024

Abstract CryoEM democratization is hampered by access to costly plunge-freezing supplies. We introduce methods, called CryoCycle, for reliably blotting, vitrifying, and reusing clipped cryoEM grids. demonstrate that vitreous ice may be produced plunging grids with purified proteins into liquid ethane reused several times different protein samples. Furthermore, we the vitrification of thin areas cells prepared on gold-coated, pre-clipped

Language: Английский

Citations

0