Materials,
Journal Year:
2024,
Volume and Issue:
17(19), P. 4765 - 4765
Published: Sept. 28, 2024
The
colonization
of
microbes
and
the
resulting
formation
biofilms
on
dental
implants
are
significant
contributors
to
peri-implantitis
failure
these
implants.
aim
research
was
analyze
impact
density
depth
laser
texturing
Ti-6Al-7Nb
alloy
surface
selected
microorganisms
biofilm
formation.
Standard
strains
Gram-negative
Gram-positive
bacteria
yeasts
from
American
Type
Culture
Collection—ATCC—were
used
demonstrate
ability
form
single-species
in
vitro.
study
evaluated
three
types
titanium
samples
with
different
texture
depth.
abilities
tested
were
assessed.
obtained
results
subjected
statistical
analysis.
Among
analyzed
strains,
L.
rhamnosus
showed
highest
surfaces.
It
found
that
there
is
no
relationship
between
parameters
number
colony-forming
units
(CFU/mL)
for
C.
albicans,
S.
mutans,
rhamnosus.
For
F.
nucleatum
strain,
it
shown
related
density.
Frontiers in Bioengineering and Biotechnology,
Journal Year:
2024,
Volume and Issue:
12
Published: Oct. 28, 2024
Background/Objective
Endoprostheses
might
fail
due
to
complications
such
as
implant
loosening
or
periprosthetic
infections.
The
surface
topography
of
materials
is
known
influence
osseointegration
and
attachment
pathogenic
bacteria.
Laser-Induced
Periodic
Surface
Structures
(LIPSS)
can
improve
the
orthopedic
materials.
In
this
preclinical
in
vitro
study,
laser
pulses
with
a
wavelength
ultraviolet
(UV)
spectrum
were
applied
for
generation
LIPSS
positively
formation
extracellular
matrix
by
primary
human
Osteoblasts
(hOBs)
reduce
microbial
biofilm
.
Methods
Laser
machining
was
employed
generating
UV-LIPSS
on
sample
disks
made
Ti6Al4V
Ti6Al7Nb
alloys.
Sample
polished
surfaces
used
controls.
Scanning
electron
microscopy
visualization
adherent
cells.
Metal
ion
release
cellular
metal
levels
investigated
inductively
coupled
plasma
mass
spectrometry.
Cell
culture
hOBs
without
treatments
performed.
Cells
their
viability,
proliferation,
osteogenic
function
cytokine
release.
Biofilm
facilitated
seeding
Staphylococcus
aureus
quantified
wheat
germ
agglutinin
(WGA)
staining.
Results
modification
results
topographies
periodicity
223
nm
≤
λ
278
nm.
ions
found
increased
decreased
Ti6Al7Nb,
while
remain
unaffected.
Cellular
adherence
when
compared
controls
proliferation
rate
Metabolic
activity
lower
control.
Alkaline
phosphatase
upregulated
grown
both
Less
pro-inflammatory
cytokines
released
cells
surfaces.
WGA
signals
significantly
indicating
reduced
S.
biofilm.
Conclusion
Our
suggest
that
texturing
bone
forming
secretion
profile
addition,
our
indicate
diminished
treated
These
effects
prove
beneficial
context
long-term
arthroplasty
outcomes.
