Evaluation of a Sample-to-Result POCKIT Central SARS-CoV-2 PCR System DOI Creative Commons
Jinhui Zhu,

Chia-Hsing Tai,

Chia-Fong Ping

et al.

Diagnostics, Journal Year: 2023, Volume and Issue: 13(13), P. 2219 - 2219

Published: June 29, 2023

The emergence of COVID-19 has caused unprecedented impacts on global public health and many other aspects. Meanwhile, types methods have been developed to detect the causative agent, SARS-CoV-2; this greatly advanced technologies in diagnostic field. Here, we describe development validation a sample-in-result-out POCKIT Central SARS-CoV-2 PCR system for detecting comparison with commercial reference real-time RT-PCR assay (TaqPath Combo Kit). Both assays were specific did not cross-react non-SARS-CoV-2 agents. able various strains including some variants. Based testing serial dilutions USA-WA1/2020 isolate, limit detection was 0.8 TCID50/mL (1.87 × 103 genomic copies/mL) 0.16 (3.75 102 PCR. Subsequently, 183 clinical samples tested by both sensitivity, specificity, agreement 91.7%, 100%, 94.0%, respectively, when compared compact sample-to-result is simplified efficient point-of-care tool detection. In addition, platform can be readily adapted human animal viruses.

Language: Английский

Optimizing drug discovery: Surface plasmon resonance techniques and their multifaceted applications DOI Creative Commons
Biswajeet Acharya, Amulyaratna Behera,

Suchismeeta Behera

et al.

Chemical Physics Impact, Journal Year: 2023, Volume and Issue: 8, P. 100414 - 100414

Published: Dec. 5, 2023

In recent years, researchers have made significant contributions to the field of Surface Plasmon Resonance sensors and their potential applications. SPR features include real-time monitoring biomolecular interactions, label-free detection, high sensitivity, ability analyze multiple samples at same time. These characteristics make biosensors a valuable tool in pharmaceutical industry for ensuring drug efficacy safety. Furthermore, reputation providing quantitative qualitative superior performance applications such as development, environmental monitoring, food safety, medical diagnostics, clinical diagnosis, biological investigations, biomolecule interactions. This review will go over advances how they can be used discovery development. We also look challenges opportunities using industry.

Language: Английский

Citations

45
Evaluation of the Multidimensional Enhanced Lateral Flow Immunoassay in Point-of-Care Nanosensors DOI
Sijie Liu,

Yangjun Liao,

Rui Shu

et al.

ACS Nano, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 23, 2024

Point-of-care (POC) nanosensors with high screening efficiency show promise for user-friendly manipulation in the ever-increasing on-site analysis demand illness diagnosis, environmental monitoring, and food safety. Currently, inspired by merits of integrating advanced nanomaterials, molecular biology, machine learning, artificial intelligence, lateral flow immunoassay (LFIA)-based POC have been devoted to satisfying commercial demands terms sensitivity, specificity, practicality. Herein, we examine use multidimensional enhanced LFIA various fields over past two decades, focusing on introducing nanomaterials improve acquisition capability small order magnitude targets through engineering transformations emphasizing interdomain fusion collaboratively address inherent challenges current applications, such as multiplexing, development detectors quantitative analysis, more practical sensitivity enhancement. Specifically, this comprehensive review encompasses latest advances comprehending an alternative signal transduction pattern, aiming achieve rapid, ultrasensitive, "sample-to-answer" available options progressive applications nanosensors. In summary, cross-collaboration disciplines, has potential break barriers toward commercialization laboratory-level nanosensors, thus leading emergence next generation LFIA.

Language: Английский

Citations

24
The Second Life for Unused COVID-19 Vaccines: Towards Biosensing Application DOI
Vincentas Mačiulis, Asta Lučiūnaitė,

Mantvydas Usvaltas

et al.

Talanta, Journal Year: 2025, Volume and Issue: unknown, P. 127647 - 127647

Published: Jan. 1, 2025

Language: Английский

Citations

1
Optical biosensing systems for a biological living body DOI Creative Commons
Tao Yan, Changfa Guo, Chunsheng Wang

et al.

View, Journal Year: 2023, Volume and Issue: 4(4)

Published: March 21, 2023

Abstract With the development of technology, biosensors are increasingly used in biomedical field. Due to high sensitivity optical signals, good stability, signal‐to‐noise ratio, and different spectral characteristics analytes, can achieve direct real‐time detection analytes with specificity compared traditional analytical techniques. In view rapid for vivo applications their promising future, we have attempted summarize working principles recent advances application humans, aim helping readers gain an in‐depth detailed understanding developed years biological living body. this review, focus on some current widely biosensors, including colorimetric fluorescence surface‐enhanced‐Raman‐scattering‐based biosensors. The each type biosensor concisely described body is summarized by category. We conclude looking at prospects

Language: Английский

Citations

14
An update on lateral flow immunoassay for the rapid detection of SARS-CoV-2 antibodies DOI Creative Commons
Lucia Spicuzza, Davide Campagna, Chiara Di Maria

et al.

AIMS Microbiology, Journal Year: 2023, Volume and Issue: 9(2), P. 375 - 401

Published: Jan. 1, 2023

Over the last three years, after outbreak of COVID-19 pandemic, an unprecedented number novel diagnostic tests have been developed. Assays to evaluate immune response SARS-CoV-2 widely considered as part control strategy. The lateral flow immunoassay (LFIA), detect both IgM and IgG against SARS-CoV-2, has studied a point-of-care (POC) test. Compared laboratory tests, LFIAs are faster, cheaper user-friendly, thus available also in areas with low economic resources. Soon onset numerous kits for rapid antibody detection were put on market emergency use authorization. However, since then, scientists tried better define accuracy these their usefulness different contexts. In fact, while during first phase pandemic auxiliary molecular diagnosis COVID-19, successively became tool seroprevalence surveillance address infection policies. When 2021 massive vaccination campaign was implemented worldwide, interest LFIA reemerged due need establish extent longevity immunization vaccinated population priorities guide health policies low-income countries limited access vaccines. Here, we summarize accuracy, advantages limits POC detection, highlighting efforts that made improve this technology over few years.

Language: Английский

Citations

14
Advancements and emerging technologies in biosensors for rapid and accurate virus detection DOI
Giovanna Marrazza, Murugan Ramalingam,

Abinaya Jaisankar

et al.

TrAC Trends in Analytical Chemistry, Journal Year: 2024, Volume and Issue: 172, P. 117609 - 117609

Published: Feb. 20, 2024

Language: Английский

Citations

6
Development and Application of an SPR Nanobiosensor Based on AuNPs for the Detection of SARS-CoV-2 on Food Surfaces DOI Creative Commons
Letícia Tessaro, Adriano Aquino, Pedro Panzenhagen

et al.

Biosensors, Journal Year: 2022, Volume and Issue: 12(12), P. 1101 - 1101

Published: Dec. 1, 2022

A new transmission route of SARS-CoV-2 through food was recently considered by the World Health Organization (WHO), and, given pandemic scenario, search for fast, sensitive, and low-cost methods is necessary. Biosensors have become a viable alternative large-scale testing because they overcome limitations standard techniques. Herein, we investigated ability gold spherical nanoparticles (AuNPs) functionalized with oligonucleotides to detect demonstrated their potential be used as plasmonic nanobiosensors. The loop-mediated isothermal amplification (LAMP) technique amplify viral genetic material from raw virus-containing solution without any preparation. detection virus presence or absence performed ultraviolet–visible (UV–Vis) absorption spectroscopy, monitoring band surface resonance (SPR) AuNPs. displacement peak 525 nm AuNPs indicated (particular region gold). On other hand, ~300 when RNA bound nanobiosensor system designed N gene in dynamic concentration range 0.1 50 × 103 ng·mL−1 limit (LOD) 1 (2.7 copy per µL), indicating excellent sensitivity. applied on surfaces vegetables showed 100% accuracy compared quantitative reverse transcription polymerase chain reaction (RT-qPCR) technique. Therefore, selective, simple, providing rapid ready-to-eat vegetables.

Language: Английский

Citations

21
Recent advances in the CRISPR/Cas system-based visual detection method DOI
Jinrong Chen, Hang Su, June Hyun Kim

et al.

Analytical Methods, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

Reviews the development of visual detection methods in recent years based on different Cas and analyzes their advantages disadvantages as well challenges future research.

Language: Английский

Citations

4
Colorimetric, Quantitative, and Portable Liquid Crystal Elastomer Biosensing of Cholesterol and Malathion DOI

Chongyang Mu,

Dawei Feng,

Mashooq Khan

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 12, 2025

Cholesteric liquid crystal elastomers (CLCEs) possess a unique characteristic that allows the manipulation of reflected color by adjusting spacing between layers. This attribute can create reliable and cost-effective colorimetric sensing platform for on-site detection various substances. In this study, CLCE films were coupled with cross-linked poly(acrylic acid) (CLCE-PAA) or poly(dimethylaminoethyl methacrylate) (CLCE-PDA) to monitor cholesterol malathion levels, respectively. both cases, change is recorded mobile phone camera, reflectance wavelength measured spectrophotometrically. For detection, smartphone application was used capture film's image, process data into hue (H) values, calculate corresponding concentration tested analyte via an analysis program. Cholesterol be detected within linear range 0.2–1.0 mM 1–10,000 ng/mL, The recoveries actual sample 86–115% 87–122% malathion, system offers practical solution testing in biological environmental samples.

Language: Английский

Citations

0
Nanotechnology-based liquid crystals used in biosensors DOI
Hazal Ezgi̇ Gülteki̇n, Merve Karpuz

Elsevier eBooks, Journal Year: 2025, Volume and Issue: unknown, P. 611 - 629

Published: Jan. 1, 2025

Language: Английский

Citations

0