A Quinolinium-Functionalized Hemicyanine Dye for Ratiometric NAD(P)H Sensing in Live Cells, Kidney Tissues, and Drosophila melanogaster DOI
Sushil K. Dwivedi,

Henry Lanquaye,

M.G.J. Waters

et al.

Sensors and Actuators B Chemical, Journal Year: 2025, Volume and Issue: unknown, P. 138043 - 138043

Published: May 1, 2025

Language: Английский

Near-Infrared Probes Designed with Hemicyanine Fluorophores Featuring Rhodamine and 1,8-Naphthalic Derivatives for Viscosity and HSA Detection in Live Cells DOI
Adenike Mary Olowolagba, Omowunmi Rebecca Aworinde, Sushil K. Dwivedi

et al.

ACS Applied Bio Materials, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 6, 2025

This paper presents the development of near-infrared (NIR) fluorescent probes,

Language: Английский

Citations

2
A wash-free fluorescent probe with red-emission for monitoring mitochondrial viscosity changes during cellular metabolism and different death processes DOI
Yuqing Wang, Kun Yu, Jing Yang

et al.

Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy, Journal Year: 2025, Volume and Issue: 333, P. 125884 - 125884

Published: Feb. 10, 2025

Language: Английский

Citations

2
AIE-based dual-ratiometric chemosensor for detection of sulfur dioxide derivatives and viscosity and its application in food samples and biological imaging DOI
Na Tang, R.‐C. SUN,

Kangshuo Xu

et al.

Analytica Chimica Acta, Journal Year: 2025, Volume and Issue: unknown, P. 343949 - 343949

Published: March 1, 2025

Language: Английский

Citations

1
A Rhodamine-Based Ratiometric Fluorescent Sensor for Dual-Channel Visible and Near-Infrared Emission Detection of NAD(P)H in Living Cells and Fruit Fly Larvae DOI

Henry Lanquaye,

Sushil K. Dwivedi, Xinzhu Li

et al.

ACS Applied Bio Materials, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 5, 2025

The detection and dynamic monitoring of intracellular NAD(P)H concentrations are crucial for comprehending cellular metabolism, redox biology, their roles in various physiological pathological processes. To address this need, we introduce sensor A, a near-infrared ratiometric fluorescent real-time, quantitative imaging fluctuations live cells. Sensor A combines 3-quinolinium electron-deficient acceptor with rhodamine dye, offering high sensitivity specificity superior photophysical properties. In its unbound state, emits strongly at 650 nm weakly 465 upon 400 excitation. Upon binding to NAD(P)H, it shows fluorescence increase decrease nm, enabling accurate measurements. also exhibits upconversion when excited 800 or 810 additional flexibility different experimental setups. sensor's response relies on the reduction by forming 1,4-dihydroquinoline donor that enhances quenches emission through photoinduced electron transfer. This mechanism ensures reliable quantification levels while minimizing interference from concentration, excitation intensity, environmental factors. was validated HeLa MD-MB453 cells under metabolic pharmacological conditions, including glucose maltose stimulation treatments chemotherapeutic agents. Co-localization mitochondrial-specific dyes confirmed mitochondrial targeting, precise tracking fluctuations. vivo Drosophila larvae nutrient starvation exposure revealed dose-dependent responses, highlighting potential changes organisms. represents significant advancement imaging, providing powerful tool exploring metabolism biology biomedical research.

Language: Английский

Citations

0
Deep-Red Cyanine-Based Fluorescent Probes with 6-Quinolinium Acceptors for Mitochondrial NAD(P)H Imaging in Live Cells and Human Diseased Kidney Tissues DOI

Dilka Liyana Arachchige,

Sushil K. Dwivedi,

Peter Agyemang

et al.

ACS Applied Bio Materials, Journal Year: 2025, Volume and Issue: unknown

Published: April 7, 2025

We developed two deep-red cyanine chromophores, probes A and B, for selective mitochondrial NAD(P)H detection in live cells. Probe features a 1,2,3,3-tetramethyl-3H-indolium core, while probe B incorporates 1,1,2,3-tetramethyl-1H-benzo[e]indol-3-ium moiety, both linked to quinolinium via vinyl bond enable fluorescence modulation upon reduction of B. To explore the role electron-withdrawing groups sensitivity, we synthesized three additional dyes (probes C, D, E) condensation 6-quinolinecarboxaldehyde with 2,3-dimethyl-1,3-benzothiazolium acceptor malononitrile derivatives, followed by methylation. Under NAD(P)H-deficient conditions, showed absorption at 382 nm weak 636 nm, absorbed 443 618 nm. Upon reduction, exhibited red-shifted 520 enhanced emission 589 550 strong 610 C 524 586 D E no detectable response, highlighting critical acceptors. demonstrated superior successfully tracking fluctuations HeLa cells under glycolysis stimulation (glucose, lactate, pyruvate) treatments LPS methotrexate. It also visualized Drosophila larvae, revealing increased levels after drug treatments. Notably, distinguished between healthy diseased human kidney tissues, detecting significantly elevated NADH autosomal dominant polycystic disease (ADPKD) samples, emphasizing its diagnostic potential. This study introduces as versatile reliable sensor metabolic research diagnostics, offering valuable insights into redox processes cells, organisms, clinical samples.

Language: Английский

Citations

0
A Quinolinium-Functionalized Hemicyanine Dye for Ratiometric NAD(P)H Sensing in Live Cells, Kidney Tissues, and Drosophila melanogaster DOI
Sushil K. Dwivedi,

Henry Lanquaye,

M.G.J. Waters

et al.

Sensors and Actuators B Chemical, Journal Year: 2025, Volume and Issue: unknown, P. 138043 - 138043

Published: May 1, 2025

Language: Английский

Citations

0