Participation of miR165a in the Phytochrome Signal Transduction in Maize (Zea mays L.) Leaves under Changing Light Conditions DOI Open Access

Д. Н. Федорин,

А. Т. Епринцев,

Victoria O. Chuykova

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(11), P. 5733 - 5733

Published: May 24, 2024

The involvement of the microRNA miR165a in light-dependent mechanisms regulation target genes maize (Zea mays) has been studied. light-induced change content free was associated with its binding by AGO10 protein and not a rate synthesis from precursor. use knockout Arabidopsis plants for phytochrome A B demonstrated that presence an active form causes increase level RNA-induced silencing complex, which triggers degradation mRNAs. two fractions vesicles leaves, P40 P100 bind miR165a, were isolated ultracentrifugation. fraction consisted larger size >0.170 µm, while <0.147 µm. Based on quantitative PCR data, predominant location surface extracellular both established. formation upon irradiation red light led to redistribution resulting proportion inside decrease vesicles.

Language: Английский

The Role of DNA Methyltransferases of Different Families in Changing the Methyl Status of DNA in Corn Leaves under Irradiation with Light of Different Wavelengths DOI

D. N. Fedorin,

А. Т. Епринцев

Russian Journal of Plant Physiology, Journal Year: 2025, Volume and Issue: 72(1)

Published: Feb. 1, 2025

Language: Английский

Citations

0
Participation of miR165a in the Phytochrome Signal Transduction in Maize (Zea mays L.) Leaves under Changing Light Conditions DOI Open Access

Д. Н. Федорин,

А. Т. Епринцев,

Victoria O. Chuykova

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(11), P. 5733 - 5733

Published: May 24, 2024

The involvement of the microRNA miR165a in light-dependent mechanisms regulation target genes maize (Zea mays) has been studied. light-induced change content free was associated with its binding by AGO10 protein and not a rate synthesis from precursor. use knockout Arabidopsis plants for phytochrome A B demonstrated that presence an active form causes increase level RNA-induced silencing complex, which triggers degradation mRNAs. two fractions vesicles leaves, P40 P100 bind miR165a, were isolated ultracentrifugation. fraction consisted larger size >0.170 µm, while <0.147 µm. Based on quantitative PCR data, predominant location surface extracellular both established. formation upon irradiation red light led to redistribution resulting proportion inside decrease vesicles.

Language: Английский

Citations

2