Biological and Ecological Roles of External Fish Mucus: A Review

Fishes, Journal Year: 2018, Volume and Issue: 3(4), P. 41 - 41

Published: Oct. 9, 2018

Fish mucus layers are the main surface of exchange between fish and environment, they possess important biological ecological functions. research is increasing rapidly, along with development high-throughput techniques, which allow simultaneous study numerous genes molecules, enabling a deeper understanding composition its plays major role against infections, has mostly focused on bioactive molecules (e.g., antimicrobial peptides immune-related molecules) associated microbiota due to their potential in aquaculture human medicine. However, external surfaces also play roles social relationships conspecifics (fish shoaling, spawning synchronisation, suitable habitat finding, or alarm signals) interspecific interactions such as prey-predator relationships, parasite–host interactions, symbiosis. This article reviews (gills skin) mucus, discussing importance protection pathogens intra interactions. We discuss advances that “omics” sciences bringing into studying

Language: Английский

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Under control: how a dietary additive can restore the gut microbiome and proteomic profile, and improve disease resilience in a marine teleostean fish fed vegetable diets

Microbiome, Journal Year: 2017, Volume and Issue: 5(1)

Published: Dec. 1, 2017

The constant increase of aquaculture production and wealthy seafood consumption has forced the industry to explore alternative more sustainable raw aquafeed materials, plant ingredients have been used replace marine feedstuffs in many farmed fish. objective present study was assess whether plant-based diets can induce changes intestinal mucus proteome, gut autochthonous microbiota disease susceptibility fish, these could be reversed by addition sodium butyrate diets. Three different trials were performed using teleostean gilthead sea bream (Sparus aurata) as model. In a first preliminary short-term trial, fish fed with additive (0.8%) supplementing basal diet low vegetable inclusion (D1) then challenged bacteria detect possible effects on survival. second greater levels (D2, D3) long-term effect at lower dose (0.4%) added D3 (D4 diet) tested proteome microbiome. third effectiveness (D4) prevent outcome after an parasite (Enteromyxum leei) challenge tested. results showed that opposed forces driven dietary supplementation diet. On one hand, induced high infection provoked drops growth performance, decreased diversity, dominance Photobacterium genus, well altered mucosal suggesting detrimental function. other slightly cumulative mortality bacterial challenge, avoided retardation parasitized increased diversity higher representation butyrate-producing most diet-induced proteome. This integrative work gives insights pleiotropic restoration homeostasis resilience, multifaceted approach.

Language: Английский

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The Inner Workings of the Outer Surface: Skin and Gill Microbiota as Indicators of Changing Gut Health in Yellowtail Kingfish

Frontiers in Microbiology, Journal Year: 2018, Volume and Issue: 8

Published: Jan. 15, 2018

The mucosal surfaces and associated microbiota of fish are an important primary barrier provide the first line defense against potential pathogens. An understanding skin gill microbial assemblages factors which drive their composition may useful insights into broad dynamics host-microbial relationships, reveal underlying changes in health status. This is particularly pertinent to cultivated systems whereby various stressors led conditions (like enteritis) impinge on productivity. As economically species, we assessed whether outer-surface bacterial communities reflect a change gut status Yellowtail Kingfish (Seriola lalandi). Active were surveyed from RNA extracts swabs gills by constructing Illumina 16S rRNA gene amplicon libraries. Proteobacteria Bacteroidetes predominant both gills, with enrichment key β-proteobacteria (Nitrosomonadales Ferrovales). Fish exhibiting early stage chronic lymphocytic enteritis comprised markedly different global compared those deemed healthy late stages disease. corresponded overall loss diversity Actinobacteria, gills. In contrast, generally similar individuals, though some distinct taxa. conclusion, factor defines likely reflects immune states during onset like enteritis. study represents investigate outer response enteritis, revealing biomarkers for assessing commercial aquaculture systems.

Language: Английский

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Immune relevant molecules identified in the skin mucus of fish using -omics technologies

Molecular BioSystems, Journal Year: 2016, Volume and Issue: 12(7), P. 2056 - 2063

Published: Jan. 1, 2016

This review will give an overview of immune relevant molecules in fish skin mucus.

Language: Английский

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Skin mucus proteome map of European sea bass (Dicentrarchus labrax)

PROTEOMICS, Journal Year: 2015, Volume and Issue: 15(23-24), P. 4007 - 4020

Published: Sept. 16, 2015

Skin mucus is the first barrier of fish defence. Proteins from skin European sea bass (Dicentrarchus labrax) were identified by 2DE followed LC-MS/MS. From all proteins in proteome map, we focus on associated with several immune pathways fish. Furthermore, real-time PCR transcript levels are shown. found include apolipoprotein A1, calmodulin, complement C3, fucose-binding lectin, lysozyme and caspases. To our knowledge, this study further transcriptional profiling done bony species. This not only contributes knowledge routes involved mucosal innate immunity, but also establishes a non-invasive technique based locating markers potential use for prevention and/or diagnosis diseases.

Language: Английский

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A chromosome-level, haplotype-resolved genome assembly and annotation for the Eurasian minnow (Leuciscidae: Phoxinus phoxinus) provide evidence of haplotype diversity

GigaScience, Journal Year: 2025, Volume and Issue: 14

Published: Jan. 1, 2025

Abstract Background In this study, we present an in-depth analysis of the Eurasian minnow (Phoxinus phoxinus) genome, highlighting its genetic diversity, structural variations, and evolutionary adaptations. We generated annotated haplotype-phased, chromosome-level genome assembly (2n = 50) by integrating high-fidelity (HiFi) long reads chromosome conformation capture data (Hi-C). Results achieved a haploid size 940 megabase pairs (Mbp) for haplome 1 929 Mbp 2 with high scaffold N50 values 36.4 Mb 36.6 BUSCO scores 96.9% 97.2%, respectively, indicating highly complete assembly. detected notable heterozygosity (1.43%) repeat content (approximately 54%), primarily consisting DNA transposons, which contribute to rearrangements variations. found substantial variations within including insertions, deletions, inversions, translocations. These affect genes enriched in functions such as dephosphorylation, developmental pigmentation, phagocytosis, immunity, stress response. annotation protein-coding genes, 30,980 messenger RNAs 23,497 were identified completeness score, further underpins contiguity our assemblies. performed gene family evolution comparing proteome 10 other teleost species, immune system families that prioritize histone-based disease prevention over NB-LRR-related-based responses. Additionally, demographic indicates historical fluctuations effective population P. phoxinus, likely correlating past climatic changes. Conclusions This annotated, phased reference provides crucial resource resolving taxonomic complexity genus Phoxinus highlights importance haplotype-phased assemblies understanding haplotype diversity species characterized heterozygosity.

Language: Английский

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Omics in fish mucosal immunity

Developmental & Comparative Immunology, Journal Year: 2017, Volume and Issue: 75, P. 99 - 108

Published: Feb. 22, 2017

Language: Английский

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Skin mucus proteins of lumpsucker (Cyclopterus lumpus)

Biochemistry and Biophysics Reports, Journal Year: 2017, Volume and Issue: 9, P. 217 - 225

Published: Jan. 6, 2017

Fish skin mucus serves as a first line of defense against pathogens and external stressors. In this study the proteomic profile lumpsucker was characterized using 2D gels coupled with tandem mass spectrometry. Mucosal proteins were identified by homology searches across databases SwissProt, NCBInr vertebrate EST. The clustered into ten groups based on their gene ontology biological process in PANTHER (www.patherdb.org). Calmodulin, cystatin-B, histone H2B, peroxiredoxin1, apolipoprotein A1, natterin-2, 14-3-3 protein, alfa enolase, pentraxin, warm temperature acclimation 65 kDa (WAP65kDa) heat shock identified. Several are known to be involved immune and/or stress responses. Proteomic established could benchmark for differential proteomics studies.

Language: Английский

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Differential proteome profile of skin mucus of gilthead seabream (Sparus aurata) after probiotic intake and/or overcrowding stress

Journal of Proteomics, Journal Year: 2015, Volume and Issue: 132, P. 41 - 50

Published: Nov. 25, 2015

Language: Английский

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Skin Mucus of Gilthead Sea Bream (Sparus aurata L.). Protein Mapping and Regulation in Chronically Stressed Fish

Frontiers in Physiology, Journal Year: 2017, Volume and Issue: 8

Published: Feb. 1, 2017

The skin mucus of gilthead sea bream was mapped by one-dimensional gel electrophoresis followed liquid chromatography coupled to high resolution mass spectrometry using a quadrupole time-of-flight analyzer. More than 2000 proteins were identified with protein score filter 30. represented in 418 canonical pathways the Ingenuity Pathway software. After filtering pathway overlapping, retained clustered three groups. mitochondrial cluster contained 59 related oxidative phosphorylation and dysfunction. second 79 antigen presentation ubiquitination pathways. third 257 where synthesis, cellular assembly, epithelial integrity over-represented. latter group also included acute phase response signaling. In parallel, two-dimensional methodology six spots different abundance when comparing unstressed fish chronically stressed an experimental model that mimicked daily farming activities. major changes associated higher cytokeratin 8 proteome fish, which confirmed immunoblotting. Thus, increased markers turnover results promising indicator chronic stress fish.

Language: Английский

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