Biological and Ecological Roles of External Fish Mucus: A Review

Fishes, Journal Year: 2018, Volume and Issue: 3(4), P. 41 - 41

Published: Oct. 9, 2018

Fish mucus layers are the main surface of exchange between fish and environment, they possess important biological ecological functions. research is increasing rapidly, along with development high-throughput techniques, which allow simultaneous study numerous genes molecules, enabling a deeper understanding composition its plays major role against infections, has mostly focused on bioactive molecules (e.g., antimicrobial peptides immune-related molecules) associated microbiota due to their potential in aquaculture human medicine. However, external surfaces also play roles social relationships conspecifics (fish shoaling, spawning synchronisation, suitable habitat finding, or alarm signals) interspecific interactions such as prey-predator relationships, parasite–host interactions, symbiosis. This article reviews (gills skin) mucus, discussing importance protection pathogens intra interactions. We discuss advances that “omics” sciences bringing into studying

Language: Английский

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Under control: how a dietary additive can restore the gut microbiome and proteomic profile, and improve disease resilience in a marine teleostean fish fed vegetable diets

Microbiome, Journal Year: 2017, Volume and Issue: 5(1)

Published: Dec. 1, 2017

The constant increase of aquaculture production and wealthy seafood consumption has forced the industry to explore alternative more sustainable raw aquafeed materials, plant ingredients have been used replace marine feedstuffs in many farmed fish. objective present study was assess whether plant-based diets can induce changes intestinal mucus proteome, gut autochthonous microbiota disease susceptibility fish, these could be reversed by addition sodium butyrate diets. Three different trials were performed using teleostean gilthead sea bream (Sparus aurata) as model. In a first preliminary short-term trial, fish fed with additive (0.8%) supplementing basal diet low vegetable inclusion (D1) then challenged bacteria detect possible effects on survival. second greater levels (D2, D3) long-term effect at lower dose (0.4%) added D3 (D4 diet) tested proteome microbiome. third effectiveness (D4) prevent outcome after an parasite (Enteromyxum leei) challenge tested. results showed that opposed forces driven dietary supplementation diet. On one hand, induced high infection provoked drops growth performance, decreased diversity, dominance Photobacterium genus, well altered mucosal suggesting detrimental function. other slightly cumulative mortality bacterial challenge, avoided retardation parasitized increased diversity higher representation butyrate-producing most diet-induced proteome. This integrative work gives insights pleiotropic restoration homeostasis resilience, multifaceted approach.

Language: Английский

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Effects of corncob derived xylooligosaccharide on innate immune response, disease resistance, and growth performance in Nile tilapia (Oreochromis niloticus) fingerlings

Aquaculture, Journal Year: 2018, Volume and Issue: 495, P. 786 - 793

Published: June 25, 2018

Language: Английский

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Comparative assessment of cortisol in plasma, skin mucus and scales as a measure of the hypothalamic-pituitary-interrenal axis activity in fish

Aquaculture, Journal Year: 2019, Volume and Issue: 506, P. 410 - 416

Published: April 2, 2019

Language: Английский

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Skin mucus metabolites and cortisol in meagre fed acute stress-attenuating diets: Correlations between plasma and mucus

Aquaculture, Journal Year: 2018, Volume and Issue: 499, P. 185 - 194

Published: Sept. 21, 2018

Language: Английский

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Comparative Immune- and Stress-Related Transcript Response Induced by Air Exposure and Vibrio anguillarum Bacterin in Rainbow Trout (Oncorhynchus mykiss) and Gilthead Seabream (Sparus aurata) Mucosal Surfaces

Frontiers in Immunology, Journal Year: 2018, Volume and Issue: 9

Published: May 2, 2018

Fish have to face various environmental challenges that may compromise the efficacy of immune response in mucosal surfaces. Since effect acute stress on barriers fish has still not been fully elucidated, we aimed compare short-term and transcriptomic responses a freshwater (rainbow trout, Oncorhynchus mykiss) marine (gilthead seabream, Sparus aurata) bacterial immersion (Vibrio anguillarum bacterin vaccine) air exposure skin, gills intestine. Air combined (vaccine+air) stressors were found be inducers cortisol secretion plasma skin mucus both species time dependent manner, while V. induced release trout only. This was coincident with marked differential increase patterns related gene expression profiles. Particularly seabream cytokines markedly enhanced, whereas mainly suppressed. In rainbow gut vaccine stimulated response, by exposure. Therefore, our comparative survey demonstrates generally more reactive species. However, upregulation transcripts pronounced vaccinated appeared stress-prone less responsive Vibrio gut. When subjected treatments no definite pattern observed. Overall, results indicate (1) homogeneous among mucosae, (2) it greatly influenced specific traits each stressor surface, (3) highly species-specific, probably as result adaptive life story microbial load characteristics their respective natural habitats.

Language: Английский

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Protein changes as robust signatures of fish chronic stress: a proteomics approach to fish welfare research

BMC Genomics, Journal Year: 2020, Volume and Issue: 21(1)

Published: April 19, 2020

Abstract Background Aquaculture is a fast-growing industry and therefore welfare environmental impact have become of utmost importance. Preventing stress associated to common aquaculture practices optimizing the fish response by quantification level, are important steps towards improvement standards. Stress characterized cascade physiological responses that, in-turn, induce further changes at whole-animal level. These can either increase fitness or impair welfare. Nevertheless, monitorization this dynamic process has, up until now, relied on indicators that only snapshot level experienced. Promising technological tools, such as proteomics, allow an unbiased approach for discovery potential biomarkers monitoring. Within scope, using Gilthead seabream ( Sparus aurata ) model, three chronic conditions, namely overcrowding, handling hypoxia, were employed evaluate protein-based adaptations reliable signatures stress, in contrast with commonly used hormonal metabolic indicators. Results A broad spectrum biological variation regarding cortisol glucose levels was observed, values which rose higher net-handled fish. In sense, pattern stressor-specificity clear, varied markedly between persistent (crowding) repetitive stressor (handling). Gel-based proteomics analysis plasma proteome also revealed had highest number differential proteins, compared other trials. Mass spectrometric analysis, followed gene ontology enrichment protein-protein interaction analyses, those humoral components innate immune system key elements stimulus. Conclusions Overall, study represents first screening more adaptation fish, allowing future development novel biomarker models monitor

Language: Английский

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Metabolic and molecular stress responses of European seabass, Dicentrarchus labrax at low and high temperature extremes

Ecological Indicators, Journal Year: 2020, Volume and Issue: 112, P. 106118 - 106118

Published: Jan. 31, 2020

Extreme water temperatures related to global climate change represent a significant challenge in terms of fish welfare and aquaculture productivity. Understanding the effect more frequent extended extreme temperature events on physiological responses, growth performance other production-relevant parameters major species are essential for developing suitable mitigation methods assuring future production. Fish were exposed 8 °C, 16 24 °C 32 evaluate ambient performance, physiological, biochemical molecular responses juvenile European seabass, Dicentrarchus labrax. 30 days exhibited significantly lower final weight, weight gain, specific rate, survival coefficient (p < 0.05) compared °C. Hepatosomatic index (HSI), viscera somatic (VSI), intestine (ISI) spleen (SSI) at day 30. Plasma [Na+] [Cl−] ion concentrations reared 10, 20 triglycerides, lactate, cortisol increased, while plasma glucose, protein liver energy storage showed inverse trend Heat shock proteins (HSP70) gene was up-regulated dorsal muscle kidney tissue Whereas Interleukin 1β (IL-1β) similar, but less regular expression with upregulation 10 across all four treatments. Insulin factor 1 (Igf1) relative decreased than seabass exhibits alterations marked reduction during None repeatedly measured current study indicated capacity compensation by adaptation over periods or days.

Language: Английский

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Extreme ambient temperature effects in European seabass, Dicentrarchus labrax: Growth performance and hemato-biochemical parameters

Aquaculture, Journal Year: 2020, Volume and Issue: 522, P. 735093 - 735093

Published: Feb. 7, 2020

Language: Английский

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Differential Reshaping of Skin and Intestinal Microbiota by Stocking Density and Oxygen Availability in Farmed Gilthead Sea Bream (Sparus aurata): A Behavioral and Network-Based Integrative Approach

Microorganisms, Journal Year: 2024, Volume and Issue: 12(7), P. 1360 - 1360

Published: July 2, 2024

Fish were kept for six weeks at three different initial stocking densities and water O2 concentrations (low-LD, 8.5 kg/m3 95–70% saturation; medium-MD, 17 55–75% high-HD, 25 60–45% saturation), with temperature increasing from 19 °C to 26–27 °C. The improvement in growth performance the decrease density was related changes skin intestinal mucosal microbiomes. Changes microbiome composition higher skin, an increased abundance of Alteromonas Massilia HD fish. However, these bacteria genera mutually exclusive, a reactive behavior systemic regulation via liver Gh/Igf system, while correlated proactive regulatory transition towards muscle rather than liver. At level, microbial showed opposite trend two taxa, rendering low Reyranella high Prauserella This up-regulated host gene expression, affecting immune response, epithelial cell turnover, abiotic stress response. Most observed responses are adaptive nature, they would serve infer new welfare indicators resilience.

Language: Английский

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