Integration of proteomics and metabolomics analysis investigate mechanism of As-induced immune injury in rat spleen DOI Creative Commons

Xiaoqian Ran,

Xi Yan,

Guanwei Ma

et al.

Ecotoxicology and Environmental Safety, Journal Year: 2024, Volume and Issue: 284, P. 116913 - 116913

Published: Aug. 28, 2024

Arsenic (As) is a widespread metalloid and human carcinogen found in the natural environment, multiple toxic effects have been shown to be associated with As exposure. can accumulated spleen, largest peripheral lymphatic organ, long-term exposure lead splenic injury. In this study, Sprague-Dawley (SD) rat model of As-poisoned was established, aiming explore molecular mechanism As-induced immune injury through combined analysis proteomics metabolomics rats' spleen. After feeding rats diet (50 mg/kg) for 90 days, spleen tissue group damaged, level significantly higher than that control (P < 0.001), inflammatory cytokine interleukin-6 (IL-6) decreased 0.01). Proteomics results showed total 134 differentially expressed proteins (DEPs) 0.05 fold change > 1.2) 182 metabolites (DEMs) (VIP >1 P 0.05) were identified spleens poisoned compared (As/Ctrl). The proteomic highlight role hypoxia-inducible factors (HIF), killer cell mediated cytotoxicity, ribosomes. major pathways metabolic disruption included arachidonic acid (AA) metabolism, glycerophospholipid metabolism folate single-carbon pool. integrated these two omics suggested Hmox1, Stat3, acid, phosphatidylcholine leukotriene B4 may play key roles by indicate cause damage rats. Through metabolomic analysis, their mechanisms obtained, which provided basis further understanding caused

Language: Английский

Mechanisms Associated with Cognitive and Behavioral Impairment Induced by Arsenic Exposure DOI Creative Commons
Gustavo Ignacio Vázquez Cervantes, Dinora F. González‐Esquivel, Daniela Ramírez Ortega

et al.

Cells, Journal Year: 2023, Volume and Issue: 12(21), P. 2537 - 2537

Published: Oct. 28, 2023

Arsenic (As) is a metalloid naturally present in the environment, food, water, soil, and air; however, its chronic exposure, even with low doses, represents public health concern. For long time, As was used as pigment, pesticide, wood preservative, for medical applications; industrial use has recently decreased or been discontinued due to toxicity. Due versatile applications distribution, there wide spectrum of human exposure sources, mainly contaminated drinking water. The fact that water implies this metalloid; it become worldwide problem, since over 200 million people live where levels exceed safe ranges. Many problems have associated including cancer, cardiovascular diseases, gastrointestinal disturbances, brain dysfunctions. Because can cross blood–brain barrier (BBB), target organ exert long-term toxic effects. mechanisms neurotoxicity described: oxidative stress, inflammation, DNA damage, mitochondrial dysfunction; all them converge, thus leading impaired cellular functions, cell death, consequence, detrimental Here, we provide current overview toxicity integrated global involved cognitive behavioral impairment induced by show experimental strategies against neurotoxicity.

Language: Английский

Citations

20
Emerging techniques for the trace elemental analysis of plants and food-based extracts: A comprehensive review DOI Creative Commons
Hemant Rawat, Shahnawaz Ahmad Bhat, Daljeet Singh Dhanjal

et al.

Talanta Open, Journal Year: 2024, Volume and Issue: 10, P. 100341 - 100341

Published: June 29, 2024

This comprehensive review investigates the dynamic landscape of trace elemental analysis methodologies applied to plants and food-based extracts. The exploration spans from inception techniques latest procedures, contributing heightened precision sensitivity in detection. According WHO, herbal medicine varied soil compositions serve as crucial therapeutic agents for 70–80 % world's population. Yet, their susceptibility element toxicity poses a significant risk human health. Rising population increased food demands have led environmental pollution, contaminating chain through unintended activities like industrialization, mining, pesticide production. composition derived extracts is central comprehending nutritional profiles, evaluating product quality, ensuring safety. Methodological advancements, progressing manual procedures sophisticated technologies such X-ray fluorescence (XRF), neutron activation (NAA), inductively coupled plasma optical emission spectroscopy (ICP OES), mass spectrometry (ICP-MS), are delineated. Recent strides paper-based electrochemical sensors highlighted distinctive capabilities elucidation associated advantages limitations. Moreover, delves into innovative sample preparation methodologies, encompassing microwave-assisted digestion solid-phase microextraction, amplify efficiency extraction subsequent analysis. Integrating data analytics machine learning elucidating complex elementary datasets explored, underscoring potential accuracy automation compiles literature data, summarizing methods various parts (roots, soil, stems, bark, fruits, food). Standard protocols United States Pharmacopeia-National Formulary (USP-FR), Ayurveda, Yoga Naturopathy, Unani, Siddha, Homeopathy (AYUSH) considered. Lead, Cadmium, Mercury, Arsenic primary toxic elements concern medicines. furnishes valuable insights tailored researchers, analysts, policymakers actively involved advancing domain extract.

Language: Английский

Citations

7
Mechanism of arsenic-induced liver injury in rats revealed by metabolomics and ionomics based approach DOI Creative Commons

Guanwei Ma,

Xi Yan,

Chao Wang

et al.

Ecotoxicology and Environmental Safety, Journal Year: 2025, Volume and Issue: 293, P. 118038 - 118038

Published: March 1, 2025

Language: Английский

Citations

0
Transgenerational Inheritance of Cognitive Deficits Induced by Ancestral Arsenic Exposure in Zebrafish (Danio rerio) via Maternal and Paternal Lineages DOI
Mahesh Rachamalla, Francisco Carlos da Silva, Markus Hecker

et al.

Environmental Science & Technology, Journal Year: 2025, Volume and Issue: unknown

Published: May 13, 2025

Exposure to arsenic is known impair learning and memory functions in animal models humans. However, the transgenerational inheritance of these cognitive deficits underlying epigenetic mechanisms remain poorly understood. The present study investigated inter- effects ancestral exposure on performance (latent learning) zebrafish via maternal paternal lineages biochemical molecular alterations brain, including DNA methylation patterns cognition-related genes. Adult male exposed dietary [30, 60, or 100 μg/g as arsenite for 90 days; F0 generation] were crossed with unexposed (control) females vice versa generate F1 progeny exposure, respectively. Subsequently, males same treatments F2 respective exposure. It was found that induced dysfunction generations both lineages. occurred at relatively lower levels (30 60 μg/g) former treatment relative those (100 latter. Inter (F1) (F2) associated concomitant elevated oxidative stress dopaminergic dysregulation, repressed expression genes such involved dopamine signaling metabolism (Drd1 MAO) brain-derived neurotrophic factor (BDNF). Furthermore, analyses revealed downregulation across three (F0 F2) resulted from hypermethylation their promotor regions (Drd1, MAO, BDNF). Collectively, observations provide novel insights into neurotoxicity.

Language: Английский

Citations

0
Environmental risk factors provoke new thinking for prevention and treatment of dementia with Lewy bodies DOI Creative Commons
Dinghao An, Yun Xu

Heliyon, Journal Year: 2024, Volume and Issue: 10(9), P. e30175 - e30175

Published: April 26, 2024

In recent years, environmental factors have received attention in the pathogenesis of neurodegenerative diseases. Other than genetic factors, identification and modifiable risk may create opportunities to delay onset or slow progression Lewy body disease. Researchers made significant progress understanding over past 30 years. To date, despite increasing number articles assessing for disease, few reviews focused on their role its onset. this review, we reviewed literature investigating relationship between disease several other factors. We found that some air pollutants, exposure metals, infection with microorganisms increase Coffee intake Mediterranean diet are protective However, it is puzzling low educational levels smoking effects. addition, proposed specific protocols subsequent research directions diseases improved methods. By conducting additional case-control studies, could explore these etiopathogenesis establishing a foundation strategies aimed at preventing reducing burden

Language: Английский

Citations

3
Effects of arsenic exposure on blood trace element levels in rats and sex differences DOI

Xiaoqian Ran,

Xi Yan,

Hongbin Zhuang

et al.

BioMetals, Journal Year: 2024, Volume and Issue: unknown

Published: April 3, 2024

Language: Английский

Citations

2
Integration of proteomics and metabolomics analysis investigate mechanism of As-induced immune injury in rat spleen DOI Creative Commons

Xiaoqian Ran,

Xi Yan,

Guanwei Ma

et al.

Ecotoxicology and Environmental Safety, Journal Year: 2024, Volume and Issue: 284, P. 116913 - 116913

Published: Aug. 28, 2024

Arsenic (As) is a widespread metalloid and human carcinogen found in the natural environment, multiple toxic effects have been shown to be associated with As exposure. can accumulated spleen, largest peripheral lymphatic organ, long-term exposure lead splenic injury. In this study, Sprague-Dawley (SD) rat model of As-poisoned was established, aiming explore molecular mechanism As-induced immune injury through combined analysis proteomics metabolomics rats' spleen. After feeding rats diet (50 mg/kg) for 90 days, spleen tissue group damaged, level significantly higher than that control (P < 0.001), inflammatory cytokine interleukin-6 (IL-6) decreased 0.01). Proteomics results showed total 134 differentially expressed proteins (DEPs) 0.05 fold change > 1.2) 182 metabolites (DEMs) (VIP >1 P 0.05) were identified spleens poisoned compared (As/Ctrl). The proteomic highlight role hypoxia-inducible factors (HIF), killer cell mediated cytotoxicity, ribosomes. major pathways metabolic disruption included arachidonic acid (AA) metabolism, glycerophospholipid metabolism folate single-carbon pool. integrated these two omics suggested Hmox1, Stat3, acid, phosphatidylcholine leukotriene B4 may play key roles by indicate cause damage rats. Through metabolomic analysis, their mechanisms obtained, which provided basis further understanding caused

Language: Английский

Citations

1