Protective effect of D‐Cys on renal function in mice with chronic kidney disease

Food Frontiers, Journal Year: 2024, Volume and Issue: 5(2), P. 558 - 569

Published: Jan. 10, 2024

Abstract MTT assay The chirality of amino acids affects their physiological functions. Recent studies uncovered potential effects D‐amino (D‐AAs) in nephropathy. Here, we explored the protective exogenous D‐AAs on chronic kidney disease (CKD). First, by 3‐(4,5‐dimethylthiazol‐2‐YI)‐2,5‐diphenyltetrazolium bromide (MTT) it was found that among four studied (D‐glutamate (D‐Glu), D‐aspartic acid (D‐Asp) being highest content fermented yogurt, and D‐alanine (D‐Ala), D‐cysteine (D‐Cys), with renal potential), D‐Cys most significantly enhanced viability hypoxia‐induced injured HK‐2 cells, even better than its L‐analog, L‐Cys. Mitochondrial function analyzed JC‐1 showed 10 100 mM can reduce green/red fluorescence intensity 16.1% ( p < .001) 17.6% .001), respectively, cells. Next, vivo effect adenine‐induced CKD mice studied. results indicated administration decreased serum creatinine urea nitrogen levels at least 15.5% 11.8%, protected mice. Further analysis induced increased water intake mice, which is beneficial for clearance 2,8‐dihydroxyadenine, thereby attenuating destruction tissue structure. Moreover, H 2 S produced from resisted oxidative stress inhibited inflammation, thus slowing down process fibrosis. In summary, this study verified structure propounded a new field application utilization D‐AAs.

Language: Английский

---

Advanced ultrasound methods to improve chronic kidney disease diagnosis

npj Imaging, Journal Year: 2024, Volume and Issue: 2(1)

Published: July 25, 2024

Abstract Chronic kidney disease (CKD) affects 850 million people worldwide and is associated with significant cardiovascular morbidity mortality. Routine laboratory tests do not reflect early stages of microcirculatory changes vascular rarefaction that characterise fibrosis, the common endpoint CKD. Imaging techniques detect CKD in could promote timely treatment new drugs like SGLT2 inhibitors, thus, decreasing progression burden. Ultrasound most used imaging modality CKD, as it non-invasive radiation free. Initially, ultrasound was applied to assess macro-morphology rule out ureteral obstruction. The development higher frequency probes allowed for more detailed parenchyma, advances Doppler provided insights into segmental arterial flow patterns including resistive indices an indirect measure impedance, elevated values which correlated progressive organ failure fibrosis. Today, low-flow detection methods matrix better resolve parenchyma smaller beds, contrast-enhanced allows perfusion measurement. Particularly, super-resolution imaging, a technology currently being clinical translation, can microcirculation morphologically functionally unrivalled detail. This accompanied by rapid developments radiomics machine learning supporting image acquisition processing, well lesion characterisation. perspective article introduces emerging diagnosis discusses how promising technical analytical advancements improve management after successful translation application.

Language: Английский

---

tRigon: an R package and Shiny App for integrative (path-)omics data analysis

BMC Bioinformatics, Journal Year: 2024, Volume and Issue: 25(1)

Published: March 5, 2024

Abstract Background Pathomics facilitates automated, reproducible and precise histopathology analysis morphological phenotyping. Similar to molecular omics, pathomics datasets are high-dimensional, but also face large outlier variability inherent data missingness, making quick comprehensible challenging. To facilitate interpretation as well support a broad implementation we developed tRigon (Toolbox foR InteGrative (path-)Omics aNalysis), Shiny application for fast, comprehensive analysis. Results is available via the CRAN repository ( https://cran.r-project.org/web/packages/tRigon ) with its source code on GitLab https://git-ce.rwth-aachen.de/labooratory-ai/trigon ). The package can be installed locally executed from R console command ‘tRigon::run_tRigon()’. Alternatively, hosted online accessed at https://labooratory.shinyapps.io/tRigon . We show fast computation of small, medium in low- high-performance hardware setting, indicating applicability tRigon. Conclusions allows researchers without coding abilities perform exploratory feature analyses non-pathomics their own using variety hardware.

Language: Английский

---

Renal IL-23–Dependent Type 3 Innate Lymphoid Cells Link Crystal-induced Intrarenal Inflammasome Activation with Kidney Fibrosis

The Journal of Immunology, Journal Year: 2024, Volume and Issue: 213(6), P. 865 - 875

Published: July 29, 2024

Chronic inflammasome activation in mononuclear phagocytes (MNPs) promotes fibrosis various tissues, including the kidney. The cellular and molecular links between are unclear. To address this question, we fed mice lacking immunological mediators an adenine-enriched diet, which causes crystal precipitation renal tubules, crystal-induced activation, fibrosis. We found that kidney depended on intrarenal inflammasome-dependent type 3 immune response driven by its signature transcription factor Rorc (retinoic acid receptor-related orphan receptor C gene), was partially carried out innate lymphoid cells (ILC3s). role of ILCs is less well known than other organs, especially ILC3. In article, describe depletion or genetic deficiency for attenuated inflammation Among inflammasome-derived cytokines, only IL-1β expanded ILC3 promoted fibrosis, whereas IL-18 caused differentiation NKp46+ Deficiency maintenance cytokine, IL-23, more protective inhibition, may be explained downregulation IL-1R, but not IL-23R, early disease, allowing persistent sensing IL-23. Mechanistically, ILC3s colocalized with MNPs vivo as shown multiepitope-ligand cartography. Cell culture experiments indicated to increase TGF-β production stimulated fibroblasts produce collagen. conclude link regulated

Language: Английский

---

Investigating the cause of cardiovascular dysfunction in chronic kidney disease: capillary rarefaction and inflammation may contribute to detrimental cardiovascular outcomes

Basic Research in Cardiology, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 30, 2024

Language: Английский

---

Soy peptides ameliorate the progression of chronic kidney disease in mice via inhibition of inflammation

Food Bioscience, Journal Year: 2023, Volume and Issue: 56, P. 103204 - 103204

Published: Sept. 28, 2023

Language: Английский

---

Polyploid tubular cells and chronic kidney disease

Kidney International, Journal Year: 2022, Volume and Issue: 102(5), P. 959 - 961

Published: Oct. 19, 2022

Language: Английский

---

Discovery of 2,8-dihydroxyadenine in HUA patients with uroliths and biomarkers for its associated nephropathy

Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, Journal Year: 2024, Volume and Issue: 1870(4), P. 167051 - 167051

Published: Feb. 7, 2024

Language: Английский

---

Deep learning applications for kidney histology analysis

Current Opinion in Nephrology & Hypertension, Journal Year: 2024, Volume and Issue: 33(3), P. 291 - 297

Published: Feb. 19, 2024

Nephropathology is increasingly incorporating computational methods to enhance research and diagnostic accuracy. The widespread adoption of digital pathology, coupled with advancements in deep learning, will likely transform our pathology practices. Here, we discuss basic concepts recent applications nephropathology, current challenges implementation future perspectives.

Language: Английский

---

Recovery or progression—AKI‑CKD transition

Deleted Journal, Journal Year: 2024, Volume and Issue: 19(3), P. 145 - 152

Published: April 6, 2024

Die akute Nierenschädigung ("acute kidney injury", AKI) ist einer der häufigsten Gründe Nierenfunktionsverschlechterung, insbesondere bei Patienten und Patientinnen im Krankenhaus. meisten Fälle eines AKI zeigen Verlauf eine gewisse Erholung Nierenfunktion, allerdings ein wichtiger Risikofaktor für die Entwicklung Progression chronischen Nierenkrankheit ("chronic disease", CKD). Eine CKD wiederum ausbleibenden nach AKI. Beide Zustände pathophysiologische Ähnlichkeiten, wie Verlust von Nephronen, Fibrosierung, Rarefizierung kleinen Blutgefäße Einwanderung Entzündungszellen, was epidemiologische Assoziation bedingen könnte. Sowohl als auch sind zudem mit erhöhten Mortalität assoziiert, unter anderem durch wechselseitige negative Beeinflussung anderen Organsystemen (sog. Organ-Crosstalk) erklärt werden kann. Zustand sollten ernst genommen AKI-Nachsorge erhalten. Hier nicht nur Kontrolle ggf. Behandlung eingeschränkten sondern mögliche Folgen an Organen dem kardiovaskulären System bedacht werden.

---