Published: Jan. 1, 2025
Language: Английский
Computational and Structural Biotechnology Journal, Journal Year: 2025, Volume and Issue: 27, P. 423 - 439
Published: Jan. 1, 2025
Antimicrobial resistance (AMR) is a major threat to global public health. The current review synthesizes address the possible role of Artificial Intelligence and Machine Learning (AI/ML) in mitigating AMR. Supervised learning, unsupervised deep reinforcement natural language processing are some main tools used this domain. AI/ML models can use various data sources, such as clinical information, genomic sequences, microbiome insights, epidemiological for predicting AMR outbreaks. Although relatively new fields, numerous case studies offer substantial evidence their successful application outbreaks with greater accuracy. These provide insights into discovery novel antimicrobials, repurposing existing drugs, combination therapy through analysis molecular structures. In addition, AI-based decision support systems real-time guide healthcare professionals improve prescribing antibiotics. also outlines how AI surveillance, analyze trends, enable early outbreak identification. Challenges, ethical considerations, privacy, model biases exist, however, continuous development methodologies enables play significant combating
Language: Английский
Citations
5
Antibiotics, Journal Year: 2025, Volume and Issue: 14(1), P. 57 - 57
Published: Jan. 9, 2025
Antimicrobial resistance is a critical global challenge in the 21st century, validating Sir Alexander Fleming's warning about misuse of antibiotics leading to resistant microbes. With dwindling arsenal effective antibiotics, it imperative concentrate on alternative antimicrobial strategies. Previous studies have not comprehensively discussed advantages and limitations various strategies, including bacteriophage therapy, probiotics, immunotherapies, photodynamic essential oils, nanoparticles peptides (AMPs) within single review. This review addresses that gap by providing an overview these non-antibiotic highlighting their pros cons, with particular emphasis (AMPs). We explore mechanism action AMPs against bacteria, viruses, fungi parasites. While hold significant promise, application mainstream drug development hindered challenges such as low bioavailability potential toxicity. However, advancements peptide engineering chemical modifications offer solutions enhance clinical utility. Additionally, this presents updates strategies aimed at improving cost, stability selective toxicity through peptidomimetics. These molecules demonstrated activity broad range pathogens, making them valuable candidates for integration into surface coatings prevent device-associated infections. Furthermore, we discuss approaches attaching functionalising surfaces. Finally, recommend comprehensive vivo evaluate efficacy mimetics, investigate synergistic combinations other assess medical devices.
Language: Английский
Citations
1
Microorganisms, Journal Year: 2025, Volume and Issue: 13(3), P. 557 - 557
Published: March 1, 2025
Antimicrobial resistance (AMR) is a growing global health crisis that threatens the efficacy of antibiotics and modern medical interventions. The emergence multidrug-resistant (MDR) pathogens, exacerbated by misuse in healthcare agriculture, underscores urgent need for innovative solutions. (1) Background: AMR arises from complex interactions between human, animal, environmental health, further aggravated overuse inadequate regulation antibiotics. Conventional treatments are increasingly ineffective, necessitating alternative strategies. Emerging approaches, including bacteriophage therapy, antimicrobial peptides (AMPs), nanotechnology, microbial extracellular vesicles (EVs), CRISPR-based antimicrobials, provide novel mechanisms complement traditional combating resistant pathogens. (2) Methods: This review critically analyzes advanced antibacterial strategies conjunction with systemic reforms such as stewardship programs, One Health framework, surveillance tools. These methods can enhance detection, guide interventions, promote sustainable practices. Additionally, economic, logistical, regulatory challenges impeding their implementation evaluated. (3) Results: technologies, CRISPR exhibit promising potential targeting mechanisms. However, disparities resource distribution barriers hinder widespread adoption. Public–private partnerships agriculture practices critical to overcoming these obstacles. (4) Conclusions: A holistic integrated approach essential mitigating impact AMR. By aligning therapeutic policies, fostering interdisciplinary collaboration, ensuring equitable distribution, we develop response this 21st-century challenge.
Language: Английский
Citations
1
Plants, Journal Year: 2025, Volume and Issue: 14(2), P. 216 - 216
Published: Jan. 14, 2025
In this work, extracts from the pulp, peel, and seed of Manilkara zapota were obtained via lyophilization oven drying. Bromatological analyses performed to investigate variabilities in nutritional content fruits after nine post-harvest days. The phytochemical was assessed by gas chromatography flame ionization detector (GC-FID), their biological performance studied using vitro antibacterial antioxidant assays (DPPH ABTS) vivo toxicity models. Molecular docking implemented evaluate interaction between polar compounds chicozapote with receptors involved pathogenesis bacterial strains. Results revealed that water or soluble solids did not vary post-harvest. It demonstrated oven-drying approaches influenced insoluble, total dietary fiber digestible carbohydrates among samples. According GC-FID analysis, it observed methods also altered myristic pentadecanoic acids extracts. noted activities weak due MIC (>1000 μg/mL) IC50 (>2000 values. Still, poor against Artemia salina nauplii. silico evaluation unveiled M. possess a high binding affinity towards DNA gyrase B cultured This study expands scientific evidence regarding influence distinct extraction on nutraceutical native importance considering additional enhance bioactivities.
Language: Английский
Citations
0
Microbiology, Journal Year: 2025, Volume and Issue: 171(1)
Published: Jan. 30, 2025
Antimicrobial resistance (AMR) is a major threat to global public health that continues grow owing selective pressure caused by the use and overuse of antimicrobial drugs. Resistance spread plasmids special concern, as they can mediate wide distribution AMR genes, including those encoding extended-spectrum β-lactamases (ESBLs). The CTX-M family ESBLs has rapidly worldwide, playing large role in declining effectiveness third-generation cephalosporins. This rapid across planet puzzling given carrying genes have been hypothesized incur fitness cost their hosts absence antibiotics. Here, we focus on WT plasmid carries bla 55 ESBL gene. We examine its conjugation rates head-to-head competitions assay associated costs both laboratory wild Escherichia coli strains. found strains exhibit intermediate levels, falling between two high-conjugation low-conjugation strains, latter being older more ancestral. also show increases lab when grown lysogeny broth Davis-Mingioli media without antibiotics, which might stem from metabolic benefits conferred host, or interactions host rifampicin-resistant mutation used marker. Laboratory displayed higher frequencies compared exception was low-passage K-12 strain, suggesting prolonged cultivation may compromised bacterial defences against plasmids. Despite low transfer among E. coli, carried minimal medium but improved enriched medium, indicating complex interplay plasmids, genetics environmental conditions. Our findings reveal an intricate relationship carriage fitness. Moreover, confer adaptive advantages beyond AMR. Altogether, these results highlight closer study dynamics critical for developing secure understanding how evolve affect adaptability necessary combating spread.
Language: Английский
Citations
0
Cureus, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 31, 2025
Antimicrobial resistance (AMR) is a growing public health issue, complicating the treatment of bacterial infections and increasing morbidity mortality globally. This phenomenon, which occurs as result ability bacteria to adapt evade conventional treatments, requires innovative strategies address it. Artificial intelligence (AI) emerges transformative tool in this context, helping accelerate identification molecules with antimicrobial potential optimize design new drugs. article analyzes usefulness AI antibiotic development, highlighting its benefits terms time, cost, efficiency fight against resistant bacteria, well challenges associated implementation biomedical field.
Language: Английский
Citations
0
The Lancet Microbe, Journal Year: 2025, Volume and Issue: unknown, P. 101069 - 101069
Published: Feb. 1, 2025
Language: Английский
Citations
0
Molecules, Journal Year: 2025, Volume and Issue: 30(4), P. 826 - 826
Published: Feb. 11, 2025
In our previous work, we showed that short antimicrobial hexapeptides (AMPs) containing three Trp and Arg residues had a potent antibiofilm activity against pathogenic Gram-positive Staphylococcus aureus MRSA strain. However, the of these Gram-negative Pseudomonas aeruginosa PA01 strain was relatively poor. Herein, tested longer 13-residue synthetic AMP tritrpticin-NH2 (Tritrp) several its analogs as potential agents can prevent biofilm formation (MBIC) and/or cause dissolution (MBEC) for two P. strains, one which expressed GFP protein. Tritrp, porcine cathelicidin, is currently only known naturally occurring cationic has in sequence (WWW), feature found to be important study. Our results show Tritrp were effective. particular, with Pro substitutions altered peptide backbone structures compared amphipathic two-turn structure more MBIC MBEC activities. Selectivity peptides towards could improved by introducing non-proteinogenic amino acid 2,3-diaminopropionic acid, rather than or Lys, positively charged residues. Using 1H NMR spectroscopy, also reinvestigated role cis–trans isomerism aqueous solution. Overall, WWW motif embedded AMPs considerable combat strains.
Language: Английский
Citations
0
Archives of Microbiology, Journal Year: 2025, Volume and Issue: 207(3)
Published: Feb. 14, 2025
Language: Английский
Citations
0
Nanomedicine, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 6
Published: Feb. 17, 2025
Currently, bacterial infection is still a major global health issue. Although antibiotics have been widely used to control and treat infections, the overuse misuse of led widespread antimicrobial resistance among many pathogens. Therefore, reducing infections through rapid accurate diagnostics crucial for public health. Traditional microbiological detection methods limitations such as poor selectivity, high complexity, excessive time consumption, highlighting urgent need develop efficient sensitive diagnosis methods. Surface-enhanced Raman spectroscopy (SERS), an emerging technique in clinical settings, holds promising future identification due its rapid, nondestructive, cost-effective nature. This invited special report discusses application SERS technology using pure culture, samples, single-cell analysis. Current challenges prospects are also addressed with in-depth discussion.
Language: Английский
Citations
0