Isolation and Characterization of Novel Escherichia coli O157:H7 Phage SPEC13 as a Therapeutic Agent for E. coli Infections In Vitro and In Vivo DOI Creative Commons
Md. Sharifull Islam, Jie Fan, Md Suzauddula

et al.

Biomedicines, Journal Year: 2024, Volume and Issue: 12(9), P. 2036 - 2036

Published: Sept. 6, 2024

O157:H7 is a recognized food-borne pathogen causing severe food poisoning at low doses. Bacteriophages (phages) are FDA-approved for use in and suggested as natural preservatives against specific pathogens. A novel phage must be identified studied to develop new preservative or antimicrobial agent

Language: Английский

The Role of Bacteriophages in the Gut Microbiota: Implications for Human Health DOI Creative Commons
Stephen Chijioke Emencheta, Chinasa Valerie Olovo, Osita C. Eze

et al.

Pharmaceutics, Journal Year: 2023, Volume and Issue: 15(10), P. 2416 - 2416

Published: Oct. 3, 2023

Bacteriophages (phages) are nano-sized viruses characterized by their inherent ability to live off bacteria. They utilize diverse mechanisms absorb and gain entry into the bacterial cell wall via release of viral genetic material, which uses replication host bacteria produce daughter progeny virions that attack surrounding cells. possess specific characteristics, including specificity for particular or closely related species. have many applications, as potential alternatives antibiotics against multi-resistant pathogens control agents in bacteria-contaminated environments. ubiquitously abundant nature biota, gut. Gut microbiota describes community interactions microorganisms within intestine. As with bacteria, parasitic bacteriophages constantly interact cells gut system obvious implications human health. However, it is imperative understand these they open up possible applicable techniques gut-implicated diseases. Thus, this review aims explore communities current impacts on

Language: Английский

Citations

22
Bacteriophages in nature: recent advances in research tools and diverse environmental and biotechnological applications DOI
Monish Bisen,

Kusum Kharga,

Sakshi Mehta

et al.

Environmental Science and Pollution Research, Journal Year: 2024, Volume and Issue: 31(15), P. 22199 - 22242

Published: Feb. 27, 2024

Language: Английский

Citations

4
An Overview of Food-Borne Pathogenic Microbes: Classification, Mode of Actions, and Recent Advances in Strategies for Management of Food-Borne Pathogens DOI
Shambhu Swarnakar, Papan Chowhan, Arka Pratim Chakraborty

et al.

Advances in Science, Technology & Innovation/Advances in science, technology & innovation, Journal Year: 2024, Volume and Issue: unknown, P. 307 - 318

Published: Jan. 1, 2024

Language: Английский

Citations

1
Characterization of GQA as a novel β-lactamase inhibitor of CTX-M-15 and KPC-2 enzymes DOI Creative Commons
Lamiaa A. Al-Madboly, Mohamed Abd El-Salam,

Jairo K. Bastos

et al.

Microbial Cell Factories, Journal Year: 2024, Volume and Issue: 23(1)

Published: Aug. 8, 2024

β-lactam resistance is a significant global public health issue. Outbreaks of bacteria resistant to extended-spectrum β-lactams and carbapenems are serious concerns that not only complicate medical care but also impact patient outcomes. The primary objective this work was express purify two soluble recombinant representative serine β‑lactamases using Escherichia coli strain as an expression host pET101/D cloning vector. Furthermore, second evaluate the potential, innovative, safe use galloylquinic acid (GQA) from Copaifera lucens potential β-lactamase inhibitor. In present study, blaCTX-M-15 blaKPC-2 represented genes encoding for β-lactamases were cloned parent isolates E. K. pneumoniae, respectively, well purification performed. Moreover, susceptibility results demonstrated cells became all test (MICs; 64–128 µg/mL) cephalosporins 128–512 µg/mL). MICs tested antibiotics determined in combination with 4 µg/mL GQA, clavulanic acid, or tazobactam against strains expressing CTX-M-15 KPC-2-β-lactamases. Interestingly, GQA resulted important reduction MIC values by 64–512-fold susceptible range comparable other reference inhibitors. Additionally, half-maximal inhibitory concentration nitrocefin substrate. Data showed agent similar efficient inhibitors enzymes, recording smaller IC50 (CTX-M-15; 17.51 tazobactam, 28.16 however, KPC-2; 20.91 24.76 GQA) compared acid. Our introduces novel non-β-lactam inhibitor, which interacts crucial residues involved recognition hydrolysis non-covalent interactions, complementing enzyme's active site. markedly enhanced potency carbapenemase β-lactamase-producing strains, reducing range. activity makes it promising lead molecule development more potent

Language: Английский

Citations

1
Effect of Escherichia coli on the photocatalytic behavior of TiO2 nanoparticles for H2 evolution from water DOI

Hui Liu,

Jiwei Zhang,

Yu-jie Luo

et al.

International Journal of Hydrogen Energy, Journal Year: 2024, Volume and Issue: 79, P. 1 - 9

Published: July 4, 2024

Language: Английский

Citations

0
Isolation and Characterization of Novel Escherichia coli O157:H7 Phage SPEC13 as a Therapeutic Agent for E. coli Infections In Vitro and In Vivo DOI Creative Commons
Md. Sharifull Islam, Jie Fan, Md Suzauddula

et al.

Biomedicines, Journal Year: 2024, Volume and Issue: 12(9), P. 2036 - 2036

Published: Sept. 6, 2024

O157:H7 is a recognized food-borne pathogen causing severe food poisoning at low doses. Bacteriophages (phages) are FDA-approved for use in and suggested as natural preservatives against specific pathogens. A novel phage must be identified studied to develop new preservative or antimicrobial agent

Language: Английский

Citations

0