Bioinformatics analysis and identification of upregulated tumor suppressor genes associated with suppressing colon cancer progression by curcumin treatment DOI Creative Commons
Dan Wu,

Zhenkai Fu,

Wenna Liu

et al.

Frontiers in Pharmacology, Journal Year: 2023, Volume and Issue: 14

Published: Sept. 5, 2023

Tumor suppressor genes (TSGs) are commonly downregulated in colon cancer and play a negative role tumorigenesis progression by affecting genomic integrity, the cell cycle, proliferation. Curcumin (CUR), Chinese herb-derived phytochemical, exerts antitumor effects on cancer. However, it remains unclear whether CUR its reactivating TSGs Here, we demonstrated that inhibited HT29 HCT116 proliferation migration cell-counting kit-8, colony-formation, wound-healing assays. Furthermore, comprehensive bioinformatics analysis of mRNA sequencing revealed 3,505 were significantly upregulated response to cells. Kyoto Encyclopedia Genes Genomes Gene Ontology analyses showed most enriched pathways containing 37 TSGs. Five ( ARHGEF12 , APAF1 VHL CEBPA CASP8 ) randomly selected for real-time fluorescence polymerase chain reaction verification results these five reactivated after treatment, suggesting related CUR-mediated inhibition. is newly identified TSG potential therapeutic target molecular docking was performed predict binding sites ARHGEF12, can prevent invasion metastasis inhibiting RhoA binding. In conclusion, present study reveals inhibits TSGs, revealing new mechanism treatment.

Language: Английский

Recent Advances in Polymer-Based Nanoformulations for Enhancing Oral Drug Delivery in Diabetes DOI
Sourbh Suren Garg,

Rajlaxmi Dey,

Atulika Sharma

et al.

Journal of Drug Delivery Science and Technology, Journal Year: 2024, Volume and Issue: 100, P. 106119 - 106119

Published: Aug. 27, 2024

Language: Английский

Citations

2
Connection between Radiation-Regulating Functions of Natural Products and miRNAs Targeting Radiomodulation and Exosome Biogenesis DOI Open Access
Jen‐Yang Tang,

Ya-Ting Chuang,

Jun‐Ping Shiau

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(15), P. 12449 - 12449

Published: Aug. 4, 2023

Exosomes are cell-derived membranous structures primarily involved in the delivery of payload to recipient cells, and they play central roles carcinogenesis metastasis. Radiotherapy is a common cancer treatment that occasionally generates exosomal miRNA-associated modulation regulate therapeutic anticancer function side effects. Combining radiotherapy natural products may modulate radioprotective radiosensitizing responses non-cancer but there knowledge gap regarding connection this combined with miRNAs their downstream targets for radiation exosome biogenesis. This review focuses on terms impacts target radiation-modulating biogenesis (secretion assembly) genes. Several have individually demonstrated miRNA-modulating However, impact natural-product-modulated response remains unclear. In review, by searching through PubMed/Google Scholar, available reports potential functions show radioprotection tissues radiosensitization among these were assessed. Next, accessing miRNA database (miRDB), predicted radiation- biogenesis-modulating genes from Gene Ontology (MGI) retrieved bioinformatically based miRNAs. Moreover, target-centric analysis showed several share same As result, miRNA-radiomodulation (radioprotection radiosensitization)-exosome axis regard natural-product-mediated radiotherapeutic effects well organized. regulating assess radiomodulation both cells cells.

Language: Английский

Citations

5
The potential therapeutic role of curcumin in osteoporosis treatment: based on multiple signaling pathways DOI Creative Commons
Keyu Wang

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15

Published: Aug. 8, 2024

Osteoporosis is a common chronic metabolic bone disease caused by disturbances in normal metabolism and an imbalance between osteoblasts osteoclasts. characterized decrease mass density, leading to increased fragility. usually treated with medications surgical methods, but these methods often produce certain side effects. Therefore, the use of traditional herbal ingredients for treatment osteoporosis has become focus attention hot topic recent years. Curcumin, widely distributed among herbs such as turmeric, tulip, curcuma longa, contains phenolic, terpenoid, flavonoid components. Modern pharmacological studies have confirmed that curcumin variety functions including antioxidant anti-inflammatory properties. In addition, positively regulates differentiation promotes proliferation osteoblasts, which play crucial role formation. Multiple shown effective it interacts signaling pathway targets, thereby interfering formation osteoclasts regulating development osteoporosis. This review summarized key pathways their mechanisms action prevention analyzed characteristics relationship curcumin. not only proves medicinal value ingredient also further elucidates molecular mechanism curcumin's anti-osteoporosis effect, providing new perspectives through multiple pathways.

Language: Английский

Citations

1
lncRNA VLDLR-AS1 Gene Expression in Colorectal Cancer in Patients from East Azerbaijan Province, Iran DOI Open Access
Amin Moqadami, Alireza Ahmadi, Mohammad Khalaj‐Kondori

et al.

Jentashapir Journal of Cellular and Molecular Biology, Journal Year: 2023, Volume and Issue: 14(4)

Published: Dec. 9, 2023

Background: Colorectal cancer (CRC) is the third most common that frequently spreads to other parts of body, with a low chance recovery and high mortality rate. Long non-coding RNAs are considered significant prognostic diagnostic indicators because they dysregulated in various cancers have distinctive expression patterns tissue- cell-specificity. VLDLR-AS1 lncRNA deregulation has been associated several malignancies. Objectives: This study aimed evaluate levels CRC. It was first time this assessment conducted. Methods: We studied 188 samples, including 94 tumor samples paired adjacent non-tumor tissues. Total RNA extracted, its quantity purity were assessed. TaKaRa PrimeScript 1st Strand cDNA Synthesis Kit (Kusatsu, Japan) used for reaction. The StepOnePlus Real-Time PCR System (Applied Biosystems) set up assess relative VLDLR-AS1. Results: According qRT-PCR data, CRC tissues significantly lower than margins (P < 0.0001). In addition, receiver operating characteristic curve analysis demonstrated can discriminate between sensitivity specificity 72.34% 51.06%, respectively = 0.03, AUC 0.6274). However, no association found clinicopathological features patients. Conclusions: results indicated downregulated patients compared healthy margin evidence shows potential gene as promising biomarker early detection development.

Language: Английский

Citations

3
Identification of phenolic compounds from inflorescences of non-psychoactive Cannabis sativa L. by UHPLC-HRMS and in vitro assessment of the antiproliferative activity against colorectal cancer DOI Creative Commons
Clarissa Caroli,

Virginia Brighenti,

Alice Cattivelli

et al.

Journal of Pharmaceutical and Biomedical Analysis, Journal Year: 2023, Volume and Issue: 236, P. 115723 - 115723

Published: Sept. 15, 2023

Phenolic compounds from Cannabis sativa L. (Cannabaceae family), in particular cannflavins, are known to possess several biological properties. However, their antiproliferative activity, being of great interest a medicinal chemistry point view, has not been deeply investigated so far the literature. In light this, aim this study was obtain an enriched fraction polyphenols (namely PEF) inflorescences non-psychoactive C. (hemp) variety and evaluate its activity against cancer cells, capitalizing on new selective extraction method for hemp polyphenols, followed by preparative flash column chromatography. Untargeted metabolomics, using based ultra-high-performance liquid chromatography coupled with high-resolution mass spectrometry (UHPLC-HRMS), applied here first time fully characterize PEF. Then, main phenolic were quantified HPLC-UV. The PEF isolated assessed vitro Caco-2 SW480 human colon adenocarcinoma cell lines providing promising IC50 values, comparison reference drug used therapy type. Based these results, can be considered as highly potential therapeutic product further colorectal cancer, thanks possible synergistic interaction compounds.

Language: Английский

Citations

2
Patient tissue-derived FGFR4-variant and wild-type colorectal cancer organoid development and anticancer drug sensitivity testing DOI Creative Commons

Hailing Lin,

Hongbo Fu,

Shishen Sun

et al.

Heliyon, Journal Year: 2024, Volume and Issue: 10(10), P. e30985 - e30985

Published: May 1, 2024

FGFR4-variant and wild-type colorectal cancer (CRC) organoids were developed to investigate the effects of FGFR4-targeted drugs, including FGFR4-IN erdafitinib, on CRC their possible molecular mechanism.

Language: Английский

Citations

0
Bioinformatics analysis and identification of upregulated tumor suppressor genes associated with suppressing colon cancer progression by curcumin treatment DOI Creative Commons
Dan Wu,

Zhenkai Fu,

Wenna Liu

et al.

Frontiers in Pharmacology, Journal Year: 2023, Volume and Issue: 14

Published: Sept. 5, 2023

Tumor suppressor genes (TSGs) are commonly downregulated in colon cancer and play a negative role tumorigenesis progression by affecting genomic integrity, the cell cycle, proliferation. Curcumin (CUR), Chinese herb-derived phytochemical, exerts antitumor effects on cancer. However, it remains unclear whether CUR its reactivating TSGs Here, we demonstrated that inhibited HT29 HCT116 proliferation migration cell-counting kit-8, colony-formation, wound-healing assays. Furthermore, comprehensive bioinformatics analysis of mRNA sequencing revealed 3,505 were significantly upregulated response to cells. Kyoto Encyclopedia Genes Genomes Gene Ontology analyses showed most enriched pathways containing 37 TSGs. Five ( ARHGEF12 , APAF1 VHL CEBPA CASP8 ) randomly selected for real-time fluorescence polymerase chain reaction verification results these five reactivated after treatment, suggesting related CUR-mediated inhibition. is newly identified TSG potential therapeutic target molecular docking was performed predict binding sites ARHGEF12, can prevent invasion metastasis inhibiting RhoA binding. In conclusion, present study reveals inhibits TSGs, revealing new mechanism treatment.

Language: Английский

Citations

1